Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	252	48.5	1661298	98.8%	1641362	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,734,706	A→G	V110A (GTG→GCG)	pgeF ←	polyphenol oxidase YfiH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,734,706	0	A	G	100.0%	127.9 / NA	39	V110A (GTG→GCG)	pgeF	polyphenol oxidase YfiH
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (21/18); total (21/18)

GGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGA  >  NC_000913/2734571‑2734841

ggCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAgg                                                                                                                                     >  2:131476/1‑140 (MQ=255)

ggCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAgg                                                                                                                                     >  2:68500/1‑140 (MQ=255)

  cccTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGcgca                                                                                                                                       >  2:746491/1‑136 (MQ=255)

        ccAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAggcagt                                                                                                                             >  1:232228/1‑140 (MQ=255)

            gCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAggcagtcggc                                                                                                                         >  2:696618/1‑140 (MQ=255)

                               tATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAAcc                                                                                                      >  2:177239/1‑140 (MQ=255)

                                        aaCAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGc                                                                                             >  2:544456/1‑140 (MQ=255)

                                         aCAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCg                                                                                            <  1:696618/140‑1 (MQ=255)

                                           aGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGt                                                                                           >  1:182836/1‑139 (MQ=255)

                                           aGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGt                                                                                           <  2:182836/139‑1 (MQ=255)

                                               aaCCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGt                                                                                           <  2:640717/135‑1 (MQ=255)

                                               aaCCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGt                                                                                           >  1:640717/1‑135 (MQ=255)

                                                  cGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCtat                                                                                   >  2:310597/1‑140 (MQ=255)

                                                    tctcTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCCCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATaa                                                                                 >  2:154148/1‑140 (MQ=255)

                                                     ctctTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAag                                                                                >  1:47731/1‑140 (MQ=255)

                                                     ctctTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAag                                                                                >  2:522705/1‑140 (MQ=255)

                                                            aGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATc                                                                         >  1:605253/1‑140 (MQ=255)

                                                                  ccTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGTGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCg                                                                   >  2:764809/1‑140 (MQ=255)

                                                                  ccTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCg                                                                   >  2:711216/1‑140 (MQ=255)

                                                                      cgcACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGtttt                                                               <  1:154148/140‑1 (MQ=255)

                                                                      cgcACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGtttt                                                               <  2:519433/140‑1 (MQ=255)

                                                                      cgcACAGTCCACGCCAGCAAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGtttt                                                               <  2:129666/140‑1 (MQ=255)

                                                                             tCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCAt                                                        <  2:232228/140‑1 (MQ=255)

                                                                                aCGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAg                                                     <  1:544456/140‑1 (MQ=255)

                                                                                     agccagcATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCg                                                >  2:179754/1‑140 (MQ=255)

                                                                                      gccagcATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGc                                               <  1:764809/140‑1 (MQ=255)

                                                                                       ccagcATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGcc                                              >  2:13068/1‑140 (MQ=255)

                                                                                       ccagcATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGcc                                              >  2:620210/1‑140 (MQ=255)

                                                                                              gAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGc                                       <  1:734596/140‑1 (MQ=255)

                                                                                                gcggcggcgACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCtt                                     <  1:711216/140‑1 (MQ=255)

                                                                                                   gcggcgACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAg                                  <  1:522705/140‑1 (MQ=255)

                                                                                                     ggcgACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGca                                >  2:743784/1‑140 (MQ=255)

                                                                                                      gcgACTTCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGcac                               >  2:453572/1‑140 (MQ=255)

                                                                                                           ttCCGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCtt                          <  1:131476/140‑1 (MQ=255)

                                                                                                             ccGTTCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTg                        <  1:177239/140‑1 (MQ=255)

                                                                                                                ttCCCGCTCGATTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCg                     <  1:620210/140‑1 (MQ=255)

                                                                                                                          aTTGCAAAACAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCt           <  1:13068/140‑1 (MQ=255)

                                                                                                                                   cAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCt           >  2:309818/1‑131 (MQ=255)

                                                                                                                                   cAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCt           <  1:309818/131‑1 (MQ=255)

                                                                                                                                   cAGCGCAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGa  <  1:453572/140‑1 (MQ=255)

GGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGA  >  NC_000913/2734571‑2734841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A