Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	252	48.5	1661298	98.8%	1641362	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,946,652	T→C	N176S (AAC→AGC)	mltA ←	membrane‑bound lytic murein transglycosylase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,946,652	0	T	C	100.0%	132.5 / NA	38	N176S (AAC→AGC)	mltA	membrane‑bound lytic murein transglycosylase A
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (20/18); total (20/18)

TCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGTTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTGGCGGCATACGGTAAATAGGATACTGGAACTCGCCC  >  NC_000913/2946520‑2946780

tCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCAt                                                                                                                           <  2:546078/140‑1 (MQ=255)

    cACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAgg                                                                                                                       <  2:596468/140‑1 (MQ=255)

            aTCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACt                                                                                                               >  1:29822/1‑140 (MQ=255)

             tCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTg                                                                                                              <  1:65483/140‑1 (MQ=255)

                   ccTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCg                                                                                                        >  2:645558/1‑140 (MQ=255)

                      tACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTg                                                                                                              <  2:739561/131‑1 (MQ=255)

                      tACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTg                                                                                                              >  1:739561/1‑131 (MQ=255)

                        ccAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAat                                                                                                   <  1:613722/140‑1 (MQ=255)

                          aaTGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAatat                                                                                                 <  2:612211/140‑1 (MQ=255)

                           aTGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAatata                                                                                                >  1:658801/1‑140 (MQ=255)

                                gcGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTAt                                                                                           <  2:658801/140‑1 (MQ=255)

                                 cGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGTCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATc                                                                                          >  1:292563/1‑140 (MQ=255)

                                  gATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCa                                                                                         <  1:714857/140‑1 (MQ=255)

                                      ggCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCa                                                                                     >  2:66899/1‑140 (MQ=255)

                                                           aTAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCg                                                                >  1:118528/1‑140 (MQ=255)

                                                               cTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACg                                                            >  2:113989/1‑140 (MQ=255)

                                                                  aaaaaGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACgaga                                                         >  2:453198/1‑140 (MQ=255)

                                                                                aCTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCAc                                           >  1:313583/1‑140 (MQ=255)

                                                                                 cTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACg                                          >  1:787868/1‑140 (MQ=255)

                                                                                    ccATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGttt                                       <  2:118528/140‑1 (MQ=255)

                                                                                     cATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGtttt                                      >  2:721358/1‑140 (MQ=255)

                                                                                       tCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTgg                                    >  2:128217/1‑140 (MQ=255)

                                                                                             aaGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTGGCGGCAt                              <  1:693849/140‑1 (MQ=255)

                                                                                             aaGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTGGCGGCAt                              <  1:453198/140‑1 (MQ=255)

                                                                                              aGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTGGCGGCATa                             <  2:787868/140‑1 (MQ=255)

                                                                                              aGTCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTGGCGGCATa                             <  1:128217/140‑1 (MQ=255)

                                                                                                tCGATATACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTGGCGGCATACg                           <  1:296277/140‑1 (MQ=255)

                                                                                                   atatACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTgg                                    <  1:73550/128‑1 (MQ=255)

                                                                                                   atatACCCACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTgg                                    >  2:73550/1‑128 (MQ=255)

                                                                                                         ccACTACCCTGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTGGCGGCATACGGTAAATAgg                  <  1:721358/140‑1 (MQ=255)

                                                                                                                  tGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTGGCGGCATACGGt                         <  2:470537/124‑1 (MQ=255)

                                                                                                                  tGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTGGCGGCATACGGt                         >  1:470537/1‑124 (MQ=255)

                                                                                                                  tGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTGGCGGCATACGGTAAATAGGATACTGGat         >  2:328888/1‑139 (MQ=255)

                                                                                                                  tGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTGGCGGCATACGGTAAATAGGATACTGGaa         >  2:58150/1‑140 (MQ=255)

                                                                                                                  tGCACATCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTGGCGGCATACGGTAAATAGGATACTGGaa         >  1:729314/1‑140 (MQ=255)

                                                                                                                       aTCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTGGCGGCATACGGTAAATAGGATACTGGAACTCGc    >  2:71072/1‑140 (MQ=255)

                                                                                                                        tCCATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTGGCGGCATACGGTAAATAGGATACTGGAACTCGcc   <  2:29822/140‑1 (MQ=255)

                                                                                                                         ccATAATGAAGCTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTGGCGGCATACGGTAAATAGGATACTGGAACTCGccc  >  2:153890/1‑140 (MQ=255)

TCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCGATATACCCACTACCCTGCACATCCATAATGAAGTTATCCATCAGGGAGTTACTGTAAGCGAGAATATATTTATCACTCAATGCCCCCGCGTAGATCTCCGCACGAGACGGCAGACGACCACGTTTTGGCGGCATACGGTAAATAGGATACTGGAACTCGCCC  >  NC_000913/2946520‑2946780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A