Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	252	48.5	1661298	98.8%	1641362	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,016,207	A→G	T50A (ACC→GCC)	ygfK →	putative oxidoreductase, Fe‑S subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,016,207	0	A	G	100.0%	185.0 / NA	54	T50A (ACC→GCC)	ygfK	putative oxidoreductase, Fe‑S subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (30/24); total (30/24)

ATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACCGTCCAAATTCTTGACCGCCTGGAGCTGGAAAA  >  NC_000913/3016072‑3016343

atGCCTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCtg                                                                                                                                      <  2:762931/140‑1 (MQ=255)

    gTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGcc                                                                                                                                  >  2:715755/1‑140 (MQ=255)

    gTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGcc                                                                                                                                  >  1:507290/1‑140 (MQ=255)

     tCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCtg                                                                                                                                      >  2:42542/1‑135 (MQ=255)

     tCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCtg                                                                                                                                      <  1:42542/135‑1 (MQ=255)

     tCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCa                                                                                                                                 <  2:507290/140‑1 (MQ=255)

     tCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCa                                                                                                                                 >  2:565977/1‑140 (MQ=255)

      cccATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCAc                                                                                                                                >  1:721205/1‑140 (MQ=255)

       ccATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACt                                                                                                                               <  2:792715/140‑1 (MQ=255)

        cATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTc                                                                                                                              >  1:463290/1‑140 (MQ=255)

                   aGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCt                                                                                                                   >  1:348957/1‑140 (MQ=255)

                         ttttGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCt                                                                                                             >  1:23061/1‑140 (MQ=255)

                         ttttGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCt                                                                                                             <  2:608212/140‑1 (MQ=255)

                            tGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGc                                                                                                          <  2:23061/140‑1 (MQ=255)

                               cgcgCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCg                                                                                                       <  2:348957/140‑1 (MQ=255)

                                  gcATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGcac                                                                                                    >  2:572413/1‑140 (MQ=255)

                                   cATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGcaca                                                                                                   >  2:557625/1‑140 (MQ=255)

                                              aaTACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTcgcg                                                                                        <  2:463290/140‑1 (MQ=255)

                                                tACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCg                                                                                                       >  1:192477/1‑123 (MQ=255)

                                                tACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCg                                                                                                       <  2:192477/123‑1 (MQ=255)

                                                 aCCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCaa                                                                                     <  1:599157/140‑1 (MQ=255)

                                                     acaacaacGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAata                                                                                 >  1:508346/1‑140 (MQ=255)

                                                     acaacaacGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAata                                                                                 >  1:348833/1‑140 (MQ=255)

                                                     acaacaacGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAata                                                                                 >  2:585584/1‑140 (MQ=255)

                                                        acaacGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTg                                                                              >  2:279605/1‑140 (MQ=255)

                                                           acGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCa                                                                           >  2:517310/1‑140 (MQ=255)

                                                             gCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACt                                                                         >  2:28037/1‑140 (MQ=255)

                                                             gCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACt                                                                         <  1:734126/140‑1 (MQ=255)

                                                             gCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACt                                                                         >  2:734126/1‑140 (MQ=255)

                                                                   tCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTgg                                                                   >  1:95288/1‑140 (MQ=255)

                                                                       tGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGa                                                               <  1:517310/140‑1 (MQ=255)

                                                                       tGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGa                                                               <  2:508346/140‑1 (MQ=255)

                                                                             tCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCg                                                         >  1:665399/1‑140 (MQ=255)

                                                                             tCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCg                                                         <  2:512841/140‑1 (MQ=255)

                                                                                 gAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACg                                                     >  1:324241/1‑140 (MQ=255)

                                                                                            ttACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAAc                                          >  2:680293/1‑140 (MQ=255)

                                                                                                    cTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAAcc                                  >  2:301370/1‑140 (MQ=255)

                                                                                                              tAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCTGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACCGTCCAAATTc                        >  2:719423/1‑140 (MQ=255)

                                                                                                               aaaaCTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAAc                                   <  1:202900/128‑1 (MQ=255)

                                                                                                               aaaaCTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAAc                                   >  2:202900/1‑128 (MQ=255)

                                                                                                                  aCTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACCGTCCAAATTCTTGa                    <  2:665399/140‑1 (MQ=255)

                                                                                                                      ttAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAAGTAAAAACCGTCCAAATTCTTGACCGc                >  1:580812/1‑140 (MQ=255)

                                                                                                                        aGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACCGTCCAAATTCTTGACCGCCt              <  2:348833/140‑1 (MQ=255)

                                                                                                                          cGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACCGTCCAAATTCTTGACCGCCTgg            <  1:565977/140‑1 (MQ=255)

                                                                                                                            tCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACCGTCCAAATTCTTGACCGCCTg             >  2:661252/1‑137 (MQ=255)

                                                                                                                            tCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACCGTCCAAATTCTTGACCGCCTg             <  1:661252/137‑1 (MQ=255)

                                                                                                                                cGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACCGTCCAAATTCTTGa                    >  2:219995/1‑126 (MQ=255)

                                                                                                                                cGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACCGTCCAAATTCTTGa                    <  1:219995/126‑1 (MQ=255)

                                                                                                                                cGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACCGTCCAAATTCTTGACCGCCTGGAGCTgg      >  1:183694/1‑140 (MQ=255)

                                                                                                                                cGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACCGTCCAAATTCTTGACCGCCTGGAGCTgg      >  2:808440/1‑140 (MQ=255)

                                                                                                                                  gTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACCGTCCAAATTCTTGACCGCCTGGAGCTGGaa    <  2:580812/140‑1 (MQ=255)

                                                                                                                                  gTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACCGTCCAAATTCTTGACCGCCTGGAGCTGGaa    <  1:301370/140‑1 (MQ=255)

                                                                                                                                  gTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACCGTCCAAATTCTTGACCGCCTGGAGCTGGaa    <  2:343754/140‑1 (MQ=255)

                                                                                                                                    gAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACCGTCCAAATTCTTGACCGCCTGGAGCTGGaaaa  <  1:28037/140‑1 (MQ=255)

ATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACCGTCCAAATTCTTGACCGCCTGGAGCTGGAAAA  >  NC_000913/3016072‑3016343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A