Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	252	48.5	1661298	98.8%	1641362	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,089,907	G→A	W51* (TGG→TGA)	yggI →	conserved protein YggI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,089,907	0	G	A	100.0%	204.2 / NA	58	W51* (TGG→TGA)	yggI	conserved protein YggI
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (27/31); total (27/31)

GCCATCCAACAGGCCGTTATGCGTCGCCTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGGCTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCA  >  NC_000913/3089779‑3090043

gCCATCCAACAGGCCGTTGTGCGTCGCCTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTa                                                                                                                               >  1:657062/1‑140 (MQ=255)

    tCCAACAGGCCGTTATGCGTCGCCTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGaa                                                                                                                           >  2:459986/1‑140 (MQ=255)

    tCCAACAGGCCGTTATGCGTCGCCTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGaa                                                                                                                           >  1:264253/1‑140 (MQ=255)

    tCCAACAGGCCGTTATGCGTCGCCTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGAAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGaa                                                                                                                           >  2:566416/1‑140 (MQ=255)

       aaCAGGCCGTTATGCGTCGCCTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAAtt                                                                                                                        <  1:231093/140‑1 (MQ=255)

       aaCAGGCCGTTATGCGTCGCCTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAAtt                                                                                                                        <  2:479926/140‑1 (MQ=255)

        aCAGGCCGTTATGCGTCGCCTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTc                                                                                                                       >  1:581466/1‑140 (MQ=255)

           ggCCGTTATGCGTCGCCTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGcc                                                                                                                    <  1:765488/140‑1 (MQ=255)

              cGTTATGCGTCGCCTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCa                                                                                                                 >  2:314574/1‑140 (MQ=255)

                      gTCGCCTGCGGGGAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGtt                                                                                                         >  2:708594/1‑140 (MQ=255)

                          ccTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGc                                                                                                     <  1:566416/140‑1 (MQ=255)

                          ccTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGc                                                                                                     <  2:792676/140‑1 (MQ=255)

                              cGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGtt                                                                                                 >  2:169448/1‑140 (MQ=255)

                                  aaaaaCTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGaa                                                                                             <  2:581466/140‑1 (MQ=255)

                                    aaaCTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGc                                                                                                     <  2:471611/130‑1 (MQ=255)

                                    aaaCTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGc                                                                                                     >  1:471611/1‑130 (MQ=255)

                                        tCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGt                                                                                       <  2:51023/140‑1 (MQ=255)

                                         cGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTg                                                                                      <  1:576357/140‑1 (MQ=255)

                                                gCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCttt                                                                               <  1:459986/140‑1 (MQ=255)

                                                  cAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTa                                                                             <  2:469661/140‑1 (MQ=255)

                                                    aCCTGAAGCTAGGGCGTAACTGCCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTAtt                                                                           <  2:483065/140‑1 (MQ=255)

                                                     ccTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTg                                                                          >  2:629336/1‑140 (MQ=255)

                                                      cTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTga                                                                         <  2:264253/140‑1 (MQ=255)

                                                      cTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTga                                                                         <  1:437694/140‑1 (MQ=255)

                                                      cTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTga                                                                         <  1:329116/140‑1 (MQ=255)

                                                      cTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTga                                                                         <  2:433400/140‑1 (MQ=255)

                                                            cTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGt                                                                   >  2:553436/1‑140 (MQ=255)

                                                             tAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTg                                                                  <  1:629336/140‑1 (MQ=255)

                                                              aGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTgg                                                                 >  2:495281/1‑140 (MQ=255)

                                                               gggCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGt                                                                >  1:525654/1‑140 (MQ=255)

                                                               gggCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCATCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGt                                                                >  2:309659/1‑140 (MQ=255)

                                                                         aCCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTgaag                                                                       >  2:145597/1‑123 (MQ=255)

                                                                         aCCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTgaag                                                                       <  1:145597/123‑1 (MQ=255)

                                                                          cccGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAAc                                                     >  1:71812/1‑140 (MQ=255)

                                                                            cGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTg                                                   <  2:71812/140‑1 (MQ=255)

                                                                               aGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGca                                                >  2:488746/1‑140 (MQ=255)

                                                                                gCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGcac                                               >  1:417719/1‑140 (MQ=255)

                                                                                 ccAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGcaca                                              <  2:525654/140‑1 (MQ=255)

                                                                                       ctctctTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCt                                        <  2:417719/140‑1 (MQ=255)

                                                                                        tctctTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTg                                       <  1:154013/140‑1 (MQ=255)

                                                                                               cacCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCt                                        >  2:16209/1‑132 (MQ=255)

                                                                                               cacCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCt                                        <  1:16209/132‑1 (MQ=255)

                                                                                               cacCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGa                                <  1:537134/140‑1 (MQ=255)

                                                                                               cacCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGa                                >  1:183949/1‑140 (MQ=255)

                                                                                                    aGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACAt                           >  2:313966/1‑140 (MQ=255)

                                                                                                    aGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACAt                           >  2:114064/1‑140 (MQ=255)

                                                                                                      cgcgGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACAttt                         >  1:779003/1‑140 (MQ=255)

                                                                                                      cgcgGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACAttt                         <  1:708594/140‑1 (MQ=255)

                                                                                                      cgcgGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTACTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACAttt                         >  1:770942/1‑140 (MQ=255)

                                                                                                           aaCCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAACCAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGcc                    >  2:212698/1‑140 (MQ=255)

                                                                                                            aCCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAACCAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCcg                   <  1:212698/140‑1 (MQ=255)

                                                                                                              cTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCcgcg                 <  2:267484/140‑1 (MQ=255)

                                                                                                                   ccgGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAgcg            <  1:313966/140‑1 (MQ=255)

                                                                                                                   ccgGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAgcg            <  1:314574/140‑1 (MQ=255)

                                                                                                                   ccgGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAgcg            <  1:488746/140‑1 (MQ=255)

                                                                                                                       aaCGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTACTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCcac        <  2:770942/140‑1 (MQ=255)

                                                                                                                         cGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACAt      >  2:65977/1‑140 (MQ=255)

                                                                                                                            ccTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGc   >  2:576197/1‑140 (MQ=255)

                                                                                                                             ctgactggAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCa  <  2:623214/140‑1 (MQ=255)

GCCATCCAACAGGCCGTTATGCGTCGCCTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGGCTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCA  >  NC_000913/3089779‑3090043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A