Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	252	48.5	1661298	98.8%	1641362	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,099,636	T→C	intergenic (‑71/+46)	yggM ← / ← ansB	DUF1202 domain‑containing protein YggM/L‑asparaginase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,099,636	0	T	C	100.0%	152.9 / NA	44	intergenic (‑71/+46)	yggM/ansB	DUF1202 domain‑containing protein YggM/L‑asparaginase 2
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/23); total (21/23)

TCTGCCCAGGCATTACCAGTCATCAACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGTGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTT  >  NC_000913/3099501‑3099771

tCTGCCCAGGCATTACCAGTCATCAACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGt                                                                                                                                     >  2:327950/1‑140 (MQ=255)

tCTGCCCAGGCATTACCAGTCATCAACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGt                                                                                                                                     <  2:579815/140‑1 (MQ=255)

   gCCCAGGCATTACCAGTCATCAACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTg                                                                                                                                  >  2:726676/1‑140 (MQ=255)

    cccAGGCATTACCAGTCATCAACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGa                                                                                                                                 >  1:489997/1‑140 (MQ=255)

        ggCATTACCAGTCATCAACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTg                                                                                                                             >  1:200838/1‑140 (MQ=255)

           aTTACCAGTCATCAACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGaaa                                                                                                                          <  1:690996/140‑1 (MQ=255)

              aCCAGTCATCAACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAg                                                                                                                       >  2:429688/1‑140 (MQ=255)

                cAGTCATCAACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGcc                                                                                                                     <  1:726676/140‑1 (MQ=255)

                  gTCATCAACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGcccc                                                                                                                   <  2:47571/140‑1 (MQ=255)

                    catcaACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCgg                                                                                                                 >  2:504509/1‑140 (MQ=255)

                      tcaACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCa                                                                                                               <  1:636871/140‑1 (MQ=255)

                      tcaACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCa                                                                                                               <  1:686285/140‑1 (MQ=255)

                      tcaACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCa                                                                                                               <  1:660327/140‑1 (MQ=255)

                      tcaACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCa                                                                                                               <  1:647521/140‑1 (MQ=255)

                      tcaACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTCGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCa                                                                                                               <  2:734217/140‑1 (MQ=255)

                       caACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCAc                                                                                                              >  2:517902/1‑140 (MQ=255)

                             aaGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATAcc                                                                                                        >  1:485356/1‑140 (MQ=255)

                                    gccgtgccAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGggcga                                                                                                 >  1:477316/1‑140 (MQ=255)

                                    gccgtgccAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGggcga                                                                                                 >  2:344028/1‑140 (MQ=255)

                                    gccgtgccAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGggcga                                                                                                 >  1:270670/1‑140 (MQ=255)

                                     ccgtgccAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGggcgag                                                                                                <  2:779965/140‑1 (MQ=255)

                                     ccgtgccAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGggcgag                                                                                                >  2:567049/1‑140 (MQ=255)

                                     ccgtgccAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGggcgag                                                                                                <  2:369232/140‑1 (MQ=255)

                                     ccgtgccAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGggcgag                                                                                                <  1:563859/140‑1 (MQ=255)

                                     ccgtgccAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGggcgag                                                                                                <  1:336915/140‑1 (MQ=255)

                                        tgccAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCa                                                                                                               <  1:728714/122‑1 (MQ=255)

                                        tgccAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCa                                                                                                               >  2:728714/1‑122 (MQ=255)

                                               aaTCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTa                                                                                      <  2:665228/140‑1 (MQ=255)

                                                          ttttCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAga                                                                           <  1:504509/140‑1 (MQ=255)

                                                             tCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAgatct                                                                        <  2:618758/140‑1 (MQ=255)

                                                               aCCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAgatctgc                                                                      <  2:485356/140‑1 (MQ=255)

                                                                            cACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCg                                                         <  1:327950/140‑1 (MQ=255)

                                                                                        tttATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCTTTGGttt                                             <  1:205148/140‑1 (MQ=255)

                                                                                             tCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCTTTGGTTTGCGTc                                        >  1:587790/1‑140 (MQ=255)

                                                                                               cGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCGGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCTTTGGTTTGCGTCag                                      >  2:740509/1‑140 (MQ=255)

                                                                                                 gTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATNTTTGGNNNGCNTCagag                                    <  2:477316/140‑1 (MQ=255)

                                                                                                 gTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCTTTGGTTTGCGTCagag                                    >  1:805570/1‑140 (MQ=255)

                                                                                                 gTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCTTTGGTTTGCGTCagag                                    <  1:740509/140‑1 (MQ=255)

                                                                                                 gTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGCCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCTTTGGTTTGCGTCagag                                    >  2:427284/1‑140 (MQ=255)

                                                                                                  tGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCTTTGGTTTGCGTCAGAGc                                   >  2:229006/1‑140 (MQ=255)

                                                                                                                  aaTTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAAcg                   >  2:816322/1‑140 (MQ=255)

                                                                                                                    ttAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAAcgcg                 >  2:665358/1‑140 (MQ=255)

                                                                                                                         aTTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCtt            >  1:702124/1‑140 (MQ=255)

                                                                                                                                   gACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCTTTGGTTTGCGTCAAAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGtt  <  2:200838/140‑1 (MQ=255)

TCTGCCCAGGCATTACCAGTCATCAACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGTGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTT  >  NC_000913/3099501‑3099771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A