Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	252	48.5	1661298	98.8%	1641362	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,391,146	T→C	T295A (ACC→GCC)	tldD ←	metalloprotease TldD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,391,146	0	T	C	100.0%	120.9 / NA	35	T295A (ACC→GCC)	tldD	metalloprotease TldD
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (17/18); total (17/18)

ACGCGCGTTGAGTTTATCCTGCATGTAGCCTTTCAGAATGCCGTTCTCAATCAGCACGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGTACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCTTCCAGACCGTGACCAACCGCTTCATGCAACAGCACGCCCGGCCAACCTGCGCCAAGTACTACC  >  NC_000913/3391009‑3391282

aCGCGCGTTGAGTTTATCCTGCATGTAGCCTTTCAGAATGCCGTTCTCAATCAGCACGTTGTACTGGCCTGGCGTACCATCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCac                                                                                                                                        >  2:804066/1‑140 (MQ=255)

 cgcgcgTTGAGTTTATCCTGCATGTAGCCTTTCAGAATGCCGTTCTCAATCAGCACGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGcaca                                                                                                                                       <  1:215545/140‑1 (MQ=255)

 cgcgcgTTGAGTTTATCCTGCATGTAGCCTTTCAGAATGCCGTTCTCAATCAGCACGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGcaca                                                                                                                                       <  2:434549/140‑1 (MQ=255)

                    gCATGTAGCCTTTCAGAATGCCGTTCTCAATCAGCACGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGc                                                                                                                    >  1:156753/1‑140 (MQ=255)

                    gCATGTAGCCTTTCAGAATGCCGTTCTCAATCAGCACGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGc                                                                                                                    >  2:565906/1‑140 (MQ=255)

                          aGCCTTTCAGAATGCCGTTCTCAATCAGCACGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCg                                                                                                              >  2:521904/1‑140 (MQ=255)

                             cTTTCAGAATGCCGTTCTCAATCAGCACGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGAcc                                                                                                           <  2:141185/140‑1 (MQ=255)

                              tttCAGAATGCCGTTCTCAATCAGCACGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCt                                                                                                          <  2:755157/140‑1 (MQ=255)

                                 cAGAATGCCGTTCTCAATCAGCACGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTc                                                                                                       >  1:795223/1‑140 (MQ=255)

                                   gAATGCCGTTCTCAATCAGCACGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCa                                                                                                     <  1:222061/140‑1 (MQ=255)

                                   gAATGCCGTTCTCAATCAGCACGTTGTACTGGCCTGGCGTACCATCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCa                                                                                                     <  1:804066/140‑1 (MQ=255)

                                     aTGCCGTTCTCAATCAGCACGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACt                                                                                                   <  1:521904/140‑1 (MQ=255)

                                         cGTTCTCAATCAGCACGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCa                                                                                                     >  1:822808/1‑134 (MQ=255)

                                         cGTTCTCAATCAGCACGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCa                                                                                                     <  2:822808/134‑1 (MQ=255)

                                             ctcAATCAGCACGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCAATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTg                                                                                           <  1:212103/140‑1 (MQ=255)

                                              tcAATCAGCACGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGa                                                                                          >  1:783101/1‑140 (MQ=255)

                                                 aTCAGCACGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGt                                                                                       <  2:483185/140‑1 (MQ=255)

                                                       aCGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCAcg                                                                                 <  1:152847/140‑1 (MQ=255)

                                                               ctggcctggcGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGa                                                                         <  2:51818/140‑1 (MQ=255)

                                                                    ctggcGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCg                                                                    <  1:97137/140‑1 (MQ=255)

                                                                       gcGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCt                                                                 >  1:785249/1‑140 (MQ=255)

                                                                       gcGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCt                                                                 >  2:153790/1‑140 (MQ=255)

                                                                       gcGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCt                                                                 >  2:581308/1‑140 (MQ=255)

                                                                         gTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTANNTACTGAAGTGCCACGGCGGTTGAAGTCGCCTTc                                                               <  1:160064/140‑1 (MQ=255)

                                                                            ccTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCTTc                                                               >  2:130240/1‑137 (MQ=255)

                                                                            ccTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCTTc                                                               <  1:130240/137‑1 (MQ=255)

                                                                             cTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCTTCCAGa                                                           >  2:52049/1‑140 (MQ=255)

                                                                             cTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGGAGCCTTCCAGa                                                           >  2:160064/1‑140 (MQ=255)

                                                                                               ccGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCTTCCAGACCGTGACCAACCGCTTCa                                         <  2:603168/140‑1 (MQ=255)

                                                                                               ccGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCTTCCAGACCGTGACCAACCGCTTCa                                         <  1:52049/140‑1 (MQ=255)

                                                                                                  aaCCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCTTCCAGACCGTGACCAACCGCTTCATGc                                      >  1:802788/1‑140 (MQ=255)

                                                                                                       cGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCTTCCAGACCGTGACCAACCGCTTCATGCAACAg                                 <  1:153790/140‑1 (MQ=255)

                                                                                                                    tCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCCTCCCGACCGCGACCCACCCCCTCCTGCAACAGCACGCCCCGCCaa                    >  2:539636/1‑140 (MQ=255)

                                                                                                                       tGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCTTCCAGACCGTGACCAACCGCTTCATGCAACAGCACGCCCGGCCAACCt                 >  1:21302/1‑140 (MQ=255)

                                                                                                                          catcatCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCTTCCAGACCGTGACCAACCGCTTCATGCAACAGCACGCCCGGCCAACCTgcg              <  1:565906/140‑1 (MQ=255)

                                                                                                                            tcatcaACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCTTCCAGACCGTGACCAACCGCTTCATGCAACAGCACGCCCGGCCAACCTGCGcc            <  1:28668/140‑1 (MQ=255)

                                                                                                                                      cGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCTTCCAGACCGGGACCAACCGCTTCATGCAACAGCACGCCCGGGCAACCTTCGCCAAGTACTAcc  >  2:143812/1‑140 (MQ=255)

ACGCGCGTTGAGTTTATCCTGCATGTAGCCTTTCAGAATGCCGTTCTCAATCAGCACGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGTACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCTTCCAGACCGTGACCAACCGCTTCATGCAACAGCACGCCCGGCCAACCTGCGCCAAGTACTACC  >  NC_000913/3391009‑3391282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A