Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	252	48.5	1661298	98.8%	1641362	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,611,596	A→G	D46D (GAT→GAC)	yhhS ←	putative transporter YhhS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,611,596	0	A	G	100.0%	167.9 / NA	48	D46D (GAT→GAC)	yhhS	putative transporter YhhS
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (25/23); total (25/23)

CGCATAAACCGAAGACGACAATCTTTTTGGGTCCCAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAGCGCGGGTTCGGCTACGGGTTCGGGC  >  NC_000913/3611460‑3611731

cGCATAAACCGAAGACGACGATCTTTTTGGGTCCCAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCcatcacgtca                                                                                                                                      <  2:172753/140‑1 (MQ=255)

   aTAAACCGAAGACGACAATCTTTTTGGGTCCCAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATgg                                                                                                                                   >  1:115143/1‑140 (MQ=255)

      aaCCGAAGACGACAATCTTTTTGGGTCCCAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACa                                                                                                                                <  2:115143/140‑1 (MQ=255)

      aaCCGAAGACGACAATCTTTTTGGGTCCCAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACa                                                                                                                                <  1:493292/140‑1 (MQ=255)

       aCCGAAGACGACAATCTTTTTGGGTCCCAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACAt                                                                                                                               <  2:553505/140‑1 (MQ=255)

          gAAGACGACAATCTTTTTGGGTCCCAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGc                                                                                                                            <  1:615443/140‑1 (MQ=255)

           aaGACGACAATCTTTTTGGGTCCCAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGcc                                                                                                                           <  1:763812/140‑1 (MQ=255)

            aGACGACAATCTTTTTGGGTCCCAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGccc                                                                                                                          >  2:659935/1‑140 (MQ=255)

                 acAATCTTTTTGGGTCCCAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACa                                                                                                                                >  1:372301/1‑129 (MQ=255)

                 acAATCTTTTTGGGTCCCAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACa                                                                                                                                <  2:372301/129‑1 (MQ=255)

                     tCTTTTTGGGTCCCAGCGAATCGGCGTAACGTCCGGTATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAg                                                                                                                             <  2:216797/128‑1 (MQ=255)

                     tCTTTTTGGGTCCCAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAg                                                                                                                             <  2:703919/128‑1 (MQ=255)

                     tCTTTTTGGGTCCCAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAg                                                                                                                             >  1:216797/1‑128 (MQ=255)

                     tCTTTTTGGGTCCCAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAg                                                                                                                             >  1:703919/1‑128 (MQ=255)

                             ggTCCCAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGTGAGCgg                                                                                                         <  2:496601/140‑1 (MQ=255)

                                cccAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCaa                                                                                                      >  1:4296/1‑140 (MQ=255)

                                 ccAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAAc                                                                                                     >  2:818991/1‑140 (MQ=255)

                                 ccAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAAc                                                                                                     >  1:540589/1‑140 (MQ=255)

                                  cAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAAcc                                                                                                    <  1:666825/140‑1 (MQ=255)

                                   aGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAAccc                                                                                                   >  2:534917/1‑140 (MQ=255)

                                       aaTCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATg                                                                                               <  2:540589/140‑1 (MQ=255)

                                                       ggCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGa                                                                               >  1:148787/1‑140 (MQ=255)

                                                              gggCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTaaa                                                                        >  2:822641/1‑140 (MQ=255)

                                                              gggCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTaaa                                                                        <  1:330069/140‑1 (MQ=255)

                                                                   gcTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGa                                                                   >  1:502636/1‑140 (MQ=255)

                                                                       aGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACtat                                                               <  2:4296/140‑1 (MQ=255)

                                                                            ggTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACAt                                                                     <  1:697682/129‑1 (MQ=255)

                                                                            ggTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACAt                                                                     >  2:697682/1‑129 (MQ=255)

                                                                                        ttGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAAt                                              >  2:160762/1‑140 (MQ=255)

                                                                                              gCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGgc                                        >  1:81028/1‑140 (MQ=255)

                                                                                                tGATAACCAGTCCTGCCCCGAATCCGCTACAGCCGATCACGACAAGGACATAGCCCGCTAATACAGCGAGCGGCAACACGATGGCGAGGCAGCTGGCGAAGTTAAACATGACTATAGAGACAAAGCGCAAATTCAGacgc                                      >  2:351995/1‑140 (MQ=255)

                                                                                                tGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGgcgc                                      <  1:822641/140‑1 (MQ=255)

                                                                                                  aTAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCaa                                    >  1:192807/1‑140 (MQ=255)

                                                                                                   tAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAAt                                   <  2:677372/140‑1 (MQ=255)

                                                                                                   tAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAAt                                   <  2:502636/140‑1 (MQ=255)

                                                                                                        aaTCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGtt                              >  2:122343/1‑140 (MQ=255)

                                                                                                        aaTCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGtt                              >  1:739624/1‑140 (MQ=255)

                                                                                                          tCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTa                            >  2:677577/1‑140 (MQ=255)

                                                                                                              gCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAgcgc                        <  1:122343/140‑1 (MQ=255)

                                                                                                              gCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAgcgc                        >  1:322453/1‑140 (MQ=255)

                                                                                                                   gAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAGCGCGGGtt                   <  2:369777/140‑1 (MQ=255)

                                                                                                                    aaGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGC‑AATTCAGGCGCAATCCGTTTAGCGCGGGTTCg                 >  1:646414/1‑140 (MQ=255)

                                                                                                                         gcTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAGCGCGGGt                    >  2:545177/1‑133 (MQ=255)

                                                                                                                         gcTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAGCGCGGGt                    <  1:545177/133‑1 (MQ=255)

                                                                                                                         gcTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAGCGCGGGTTCGGCTa             >  2:455317/1‑140 (MQ=255)

                                                                                                                         gcTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAGCGCGGGTTCGGCTa             <  2:322453/140‑1 (MQ=255)

                                                                                                                              aGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAGCGCGGGTTCGGCTACGGGt        >  2:424577/1‑140 (MQ=255)

                                                                                                                              aGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAGCGCGGGTTCGGCTACGGGt        >  2:110997/1‑140 (MQ=255)

                                                                                                                               gCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAGCGCGGGTTCGGCTACGGGtt       <  1:677577/140‑1 (MQ=255)

                                                                                                                                 ccATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGC‑AATTCAGGCGCAATCCGTTTAGCGCGGGTTCGGCTACGGGTTCgg    <  2:646414/140‑1 (MQ=255)

                                                                                                                                    tcacgtcaTGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAGCGCGGGTTCGGCTACGGGTTCGGGc  <  1:455317/140‑1 (MQ=255)

CGCATAAACCGAAGACGACAATCTTTTTGGGTCCCAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAGCGCGGGTTCGGCTACGGGTTCGGGC  >  NC_000913/3611460‑3611731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A