Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	145	97.8	3401598	99.2%	3374385	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,154,161	G→A	G49D (GGT→GAT)	mdtA →	multidrug efflux pump membrane fusion protein MdtA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,154,161	0	G	A	100.0%	116.5 / NA	34	G49D (GGT→GAT)	mdtA	multidrug efflux pump membrane fusion protein MdtA
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (25/9); total (25/9)

GTTATAAATCCCGTTGGGTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGGTGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGTTCAGGCGGCGACCGCCGTAGAACAGGCAGTTCCGCGTTACCTCACCGGGCTTGGCACCATTACCGCCGCTAATACCGTTACGGTGCGCAGCCGCG  >  NC_000913/2154026‑2154295

gTTATAAATCCCGTTGGGTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGgatggt                                                                                                                                    <  2:141150/140‑1 (MQ=255)

 ttATAAATCCCGTTGGGTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTc                                                                                                                                   >  2:1557562/1‑140 (MQ=255)

     aaaTCCCGTTGGGTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACg                                                                                                                               <  1:1557562/140‑1 (MQ=255)

      aaTCCCGTTGGGTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGgatggt                                                                                                                                    >  2:1360397/1‑134 (MQ=255)

      aaTCCCGTTGGGTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGgatggt                                                                                                                                    <  1:1360397/134‑1 (MQ=255)

      aaTCCCGTTGGGTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGt                                                                                                                              <  2:990999/140‑1 (MQ=255)

       aTCCCGTTGGGTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTg                                                                                                                             >  2:426911/1‑140 (MQ=255)

       aTCCCGTTGGGTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTg                                                                                                                             >  2:927563/1‑140 (MQ=255)

        tCCCGTTGGGTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGt                                                                                                                              >  1:1401508/1‑138 (MQ=255)

        tCCCGTTGGGTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGt                                                                                                                              <  2:1401508/138‑1 (MQ=255)

        tCCCGTTGGGTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTgg                                                                                                                            >  2:360743/1‑140 (MQ=255)

           cGTTGGGTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTAt                                                                                                                         >  2:1591384/1‑140 (MQ=255)

           cGTTGGGTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTAt                                                                                                                         >  1:868231/1‑140 (MQ=255)

              tGGGTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGgatgg                                                                                                                                     >  1:152575/1‑125 (MQ=255)

              tGGGTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGgatgg                                                                                                                                     <  2:152575/125‑1 (MQ=255)

               gggTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGt                                                                                                                     <  1:1591384/140‑1 (MQ=255)

                  taatcgtaatcgTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTcc                                                                                                                  <  1:927563/140‑1 (MQ=255)

                        taatcgtGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCa                                                                                                            <  1:1476928/140‑1 (MQ=255)

                              tggtggTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGcc                                                                                                      <  1:426911/140‑1 (MQ=255)

                               ggtggtTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGccc                                                                                                     >  1:1159880/1‑140 (MQ=255)

                               ggtggtTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGccc                                                                                                     >  1:879877/1‑140 (MQ=255)

                               ggtggtTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGccc                                                                                                     >  1:792659/1‑140 (MQ=255)

                               ggtggtTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGccc                                                                                                     >  1:1380126/1‑140 (MQ=255)

                                gtggtTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGcccc                                                                                                    >  1:61037/1‑140 (MQ=255)

                                   gtTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGt                                                                                                 >  1:410620/1‑140 (MQ=255)

                                    ttATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGtt                                                                                                <  1:360743/140‑1 (MQ=255)

                                          ccgccATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGTTCAggcg                                                                                          <  2:1357089/140‑1 (MQ=255)

                                             ccATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGTTCAGgcggcg                                                                                       >  1:13779/1‑140 (MQ=255)

                                               acccccgcATTCTGGTTCGGGCAAGGCCGAAATGCCCCCCGGGGGGCAGCCCGGGGGGCGACAAAACAAGCGCAGCAACCGCCAGGGGATGGTCGACGTGGTATGCGTCCCGCCCCATTAGCCCCGTTTCAGGCGGCGAc                                                                                     <  2:792659/136‑1 (MQ=255)

                                                                     aaGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGTTCAGGCGGCGACCGCCGTAGAACAGGCAGTTCcg                                                               >  2:968469/1‑140 (MQ=255)

                                                                               tgccccccGGAGTGCAGCCCCGGGGGCGACAAAACAAGCGCACCAACCGCCAGGGGATGGTCGACGTGGTATGCGTCCCGGCCCATTACCCCCGGTTCGGGCGGCGACCGCCGTAGAACAGGCAGTTCCGCGTTACCTCa                                                     <  2:1488806/135‑1 (MQ=255)

                                                                                 aCTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGTTCAGGCGGCGACCGCCGTAGAACAGGCAGTTCCGCGTTACCTCAcc                                                   >  1:1488806/1‑140 (MQ=255)

                                                                                 aCTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGTTCAGGCGGCGACCGCCGTAGAACAGGCAGTTCCGCGTTACCTCAcc                                                   >  1:755332/1‑140 (MQ=255)

                                                                                 aCTCCCAGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGTTCAGGCGGCGACCGCCGTAGAACAGGCAGTTCCGCGTTACCTCAcc                                                   >  1:866473/1‑140 (MQ=255)

                                                                                   tCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGTTCAGGCGGCGACCGCCGTAGAACAGGCAGTTCCGCGTTACCTCACCgg                                                 >  2:341113/1‑140 (MQ=255)

                                                                                             cAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGTTCAGGCGGCGACCGCCGTAGAACAGGCAGTTCCGCGTTACCTCACCGGGCTTGGCAcc                                       >  1:576212/1‑140 (MQ=255)

                                                                                             cAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGTTCAGGCGGCGACCGCCGTAGAACAGGCAGTTCCGCGTTACCTCACCGGGCTTGGCAcc                                       >  1:434646/1‑140 (MQ=255)

                                                                                                     ggggCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGTTCAGGCGGCGACCGCCGTAGAACAGGCAGTTCCGCGTTACCTCACCGGGc                                               >  1:918304/1‑124 (MQ=255)

                                                                                                     ggggCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGTTCAGGCGGCGACCGCCGTAGAACAGGCAGTTCCGCGTTACCTCACCGGGc                                               <  2:918304/124‑1 (MQ=255)

                                                                                                     ggggCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGTTCAGGCGGCGACCGCCGTAGAACAGGCAGTTCCGCGTTACCTCACCGGGCTTGGCACCATTAccgc                               >  1:1568914/1‑140 (MQ=255)

                                                                                                     ggggCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGTTCAGGCGGCGACCGCCGTAGAACAGGCAGTTCCGCGTTACCTCACCGGGCTTGGCACCATTAccgc                               >  1:1306808/1‑140 (MQ=255)

                                                                                                                                  aGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGTTCAGGCGGCGACCGCCGTAGAACAGGCAGTTCCGCGTTACCTCACCGGGCTTGGCACCATTACCGCCGCTAATACCGTTACGGTGCGCAGCcgcg  >  1:112611/1‑140 (MQ=255)

GTTATAAATCCCGTTGGGTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGGTGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGTTCAGGCGGCGACCGCCGTAGAACAGGCAGTTCCGCGTTACCTCACCGGGCTTGGCACCATTACCGCCGCTAATACCGTTACGGTGCGCAGCCGCG  >  NC_000913/2154026‑2154295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A