Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	145	97.8	3401598	99.2%	3374385	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,167,173	T→C	noncoding (60/87 nt)	cyaR →	small regulatory RNA CyaR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,167,173	0	T	C	98.6%	252.4 / ‑4.4	71	noncoding (60/87 nt)	cyaR	small regulatory RNA CyaR
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (40/30); total (41/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

CGATTATTTTTCCCGGATGGAAAATTCTTAGAAACCGATCACATACAGCTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATC  >  NC_000913/2167037‑2167308

cGATTATTTTTCCCGGATGGAAAATTCTTAGAAACCGATCACATACAGCTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAAt                                                                                                                                      <  1:435510/140‑1 (MQ=255)

cGATTATTTTTCCCGGATGGAAAATTCTTAGAAACCGATCACATACAGCTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAAt                                                                                                                                      <  2:1183025/140‑1 (MQ=255)

cGATTATTTTTCCCGGATGGAAAATTCTTAGAAACCGATCACATACAGCTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAAt                                                                                                                                      <  2:128701/140‑1 (MQ=255)

                        ttCTTAGAAACCGATCACATACAGCTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTAtttttt                                                                                                              <  1:1076029/140‑1 (MQ=255)

                        ttCTTAGAAACCGATCACATACAGCTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTAtttttt                                                                                                              <  2:490685/140‑1 (MQ=255)

                        ttCTTAGAAACCGATCACATACAGCTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTAtttttt                                                                                                              <  2:1665548/140‑1 (MQ=255)

                           ttAGAAACCGATCACATACAGCTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTAcg                                                                                                           <  1:656962/140‑1 (MQ=255)

                            tAGAAACCGATCACATACAGCTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTAcgc                                                                                                          >  2:1629728/1‑140 (MQ=255)

                             aGAAACCGATCACATACAGCTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTAcgcg                                                                                                         <  2:1105098/140‑1 (MQ=255)

                                     aTCACATACAGCTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACaa                                                                                                 >  1:197104/1‑140 (MQ=255)

                                          aTACAGCTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGaaa                                                                                            <  2:1230805/140‑1 (MQ=255)

                                              aGCTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATaaa                                                                                        <  2:197104/140‑1 (MQ=255)

                                               gCTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATaaaa                                                                                       <  1:1071121/140‑1 (MQ=255)

                                               gCTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATaaaa                                                                                       >  2:1071121/1‑140 (MQ=255)

                                               gCTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATaaaa                                                                                       <  2:1216097/140‑1 (MQ=255)

                                                cTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAAt                                                                                                >  2:1108036/1‑130 (MQ=255)

                                                cTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAAt                                                                                                <  1:1108036/130‑1 (MQ=255)

                                                cTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAg                                                                                      >  2:155543/1‑140 (MQ=255)

                                                cTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAg                                                                                      >  1:1490999/1‑140 (MQ=255)

                                                cTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAg                                                                                      >  1:1218798/1‑140 (MQ=255)

                                                cTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAg                                                                                      >  2:382890/1‑140 (MQ=255)

                                                cTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAg                                                                                      >  2:500240/1‑140 (MQ=255)

                                                cTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAg                                                                                      >  1:259632/1‑140 (MQ=255)

                                                   cATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGat                                                                                   >  1:932156/1‑140 (MQ=255)

                                                   cATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGat                                                                                   >  1:1528141/1‑140 (MQ=255)

                                                    aTTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACaa                                                                                                 <  2:1150425/125‑1 (MQ=255)

                                                    aTTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACaa                                                                                                 >  1:1150425/1‑125 (MQ=255)

                                                    aTTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGatt                                                                                  >  2:1141443/1‑140 (MQ=255)

                                                    aTTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGatt                                                                                  >  1:1253892/1‑140 (MQ=255)

                                                     tttATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGattt                                                                                 >  2:1636438/1‑140 (MQ=255)

                                                         ttaAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGatttattt                                                                             <  2:932156/140‑1 (MQ=255)

                                                          taAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTc                                                                            >  2:1138611/1‑140 (MQ=255)

                                                             ggTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTgg                                                                         >  2:497252/1‑140 (MQ=255)

                                                                tATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCa                                                                      <  1:497252/140‑1 (MQ=255)

                                                                 atcatcCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGt                                                                        <  2:1366833/137‑1 (MQ=255)

                                                                 atcatcCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGt                                                                        >  1:1366833/1‑137 (MQ=255)

                                                                  tcatcCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACg                                                                    >  1:1343092/1‑140 (MQ=255)

                                                                  tcatcCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCATACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACg                                                                    >  2:1516542/1‑140 (MQ=255)

                                                                   catcCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGt                                                                   >  1:1446074/1‑140 (MQ=255)

                                                                       cGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCcac                                                               >  1:334141/1‑140 (MQ=255)

                                                                         tttCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCcacac                                                             >  2:1437740/1‑140 (MQ=255)

                                                                         tttCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCcacac                                                             >  1:144356/1‑140 (MQ=255)

                                                                         tttCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCcacac                                                             <  1:1138611/140‑1 (MQ=255)

                                                                            cGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACAtt                                                          >  1:940058/1‑140 (MQ=255)

                                                                              cTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGa                                                        >  2:1205275/1‑140 (MQ=255)

                                                                                             cATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACaaaaa                                         <  1:1380518/140‑1 (MQ=255)

                                                                                                 aaaTGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGcc                                     >  2:1070666/1‑140 (MQ=255)

                                                                                                    tGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCt                                  <  1:1533377/140‑1 (MQ=255)

                                                                                                    tGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCt                                  <  1:1516542/140‑1 (MQ=255)

                                                                                                    tGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGACACGTCCACACATTGACCACATCGACAAAAAAGCCCCt                                  >  1:804358/1‑140 (MQ=255)

                                                                                                     gCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCt                                  >  1:647662/1‑139 (MQ=255)

                                                                                                     gCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCt                                  <  2:647662/139‑1 (MQ=255)

                                                                                                     gCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTc                                 >  1:1383457/1‑140 (MQ=255)

                                                                                                        aGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGAc                              >  2:1028828/1‑140 (MQ=255)

                                                                                                        aGCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGAc                              >  2:263058/1‑140 (MQ=255)

                                                                                                         gCTGTACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACt                             >  1:229146/1‑140 (MQ=255)

                                                                                                             tACCAGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAgg                         >  2:640115/1‑140 (MQ=255)

                                                                                                                 aGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCt                     <  2:1383457/140‑1 (MQ=255)

                                                                                                                 aGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCt                     >  2:656243/1‑140 (MQ=255)

                                                                                                                 aGGAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCt                     >  2:1345976/1‑140 (MQ=255)

                                                                                                                   gAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTc                   <  1:1345976/140‑1 (MQ=255)

                                                                                                                   gAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTc                   <  1:656243/140‑1 (MQ=255)

                                                                                                                   gAACCACCTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTc                   <  1:1159584/140‑1 (MQ=255)

                                                                                                                     accaccTCCTTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCt                 <  1:258096/140‑1 (MQ=255)

                                                                                                                         cctcctTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGttt             <  1:1629728/140‑1 (MQ=255)

                                                                                                                          ctcctTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTc            >  2:1067037/1‑140 (MQ=255)

                                                                                                                             ctTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTaa         >  1:1245698/1‑140 (MQ=255)

                                                                                                                             ctTAGCCTGTGCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTaa         <  1:1067037/140‑1 (MQ=255)

                                                                                                                                    tgtgCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGttt             <  1:432151/129‑1 (MQ=255)

                                                                                                                                    tgtgCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGttt             >  2:432151/1‑129 (MQ=255)

                                                                                                                                    tgtgCAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATc  >  1:87846/1‑140 (MQ=255)

CGATTATTTTTCCCGGATGGAAAATTCTTAGAAACCGATCACATACAGCTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATC  >  NC_000913/2167037‑2167308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A