Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	145	97.8	3401598	99.2%	3374385	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,446,776	G→A	A233V (GCG→GTG)	aroC ←	chorismate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,446,776	0	G	A	100.0%	128.4 / NA	37	A233V (GCG→GTG)	aroC	chorismate synthase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (13/24); total (13/24)

CGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCGCATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGCAACAACGGTGACTTTAGCGCCGATGGAGTCGCCCTCTTTTTTCAGCGCACGCATCAACTCGTCTAA  >  NC_000913/2446642‑2446909

cgAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATgg                                                                                                                                  >  1:1125079/1‑140 (MQ=255)

 gagaATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATggg                                                                                                                                 <  2:230751/140‑1 (MQ=255)

   gaATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCg                                                                                                                               <  2:1390246/140‑1 (MQ=255)

   gaATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCg                                                                                                                               >  2:842495/1‑140 (MQ=255)

       gccgccCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGt                                                                                                                           <  2:687527/140‑1 (MQ=255)

           cccgcATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGc                                                                                                                       >  1:1176880/1‑140 (MQ=255)

                  ggTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAgg                                                                                                                <  1:155646/140‑1 (MQ=255)

                  ggTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAgg                                                                                                                <  2:1445657/140‑1 (MQ=255)

                  ggTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAgg                                                                                                                <  1:842495/140‑1 (MQ=255)

                      gCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGt                                                                                                            <  2:110148/140‑1 (MQ=255)

                        tctGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCa                                                                                                          <  1:501505/140‑1 (MQ=255)

                                  gTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCt                                                                                                >  1:1325723/1‑140 (MQ=255)

                                       tGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCa                                                                                           <  1:836678/140‑1 (MQ=255)

                                              ttCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCgg                                                                                    >  1:444312/1‑140 (MQ=255)

                                               tCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGc                                                                                   <  1:317265/140‑1 (MQ=255)

                                                 aTCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAAACCGGCTCGGCAAGGCCCGccc                                                                                 >  2:1119887/1‑138 (MQ=255)

                                                         tCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCa                                                                         <  2:1416467/140‑1 (MQ=255)

                                                          cTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCAc                                                                        >  1:620653/1‑140 (MQ=255)

                                                              cTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGc                                                                    <  1:1118204/140‑1 (MQ=255)

                                                               tGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGca                                                                   <  2:261936/140‑1 (MQ=255)

                                                                gCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGcaa                                                                  <  2:620653/140‑1 (MQ=255)

                                                                    cgcAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCa                                                                         >  2:1002118/1‑129 (MQ=255)

                                                                    cgcAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCa                                                                         <  1:1002118/129‑1 (MQ=255)

                                                                     gcAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGCAACAACg                                                             <  2:1158357/140‑1 (MQ=255)

                                                                             accacGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGCAACAACGGTGACttt                                                     <  2:482024/140‑1 (MQ=255)

                                                                                            tCGCCAATTTCTACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGCAACAACGGTGACTTTAGCGCCGATGGAGTc                                      >  1:1153938/1‑140 (MQ=255)

                                                                                            tCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGCAACAACGGTGACTTTAGCGCCGATGGAGTc                                      >  1:274165/1‑140 (MQ=255)

                                                                                              gCCAATTTCTACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGCAACAACGGTGACTTTAGCGCCGATGGAGTCGc                                    <  2:1153938/140‑1 (MQ=255)

                                                                                                         cGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGCAACAACGGGGACTTTAGCGCCGATGGAGTCGCCCTCTTTTTTc                         >  1:556326/1‑140 (MQ=255)

                                                                                                             tttCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGCAACAACGGTGACTTTAGCGCCGATGGAGTCGCCCTCTTTTTTCAgcg                     <  2:1176880/140‑1 (MQ=255)

                                                                                                              ttCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGCAACAACGGTGACTTTAGCGCCGATGGAGTCGCCCTCTTTTTTCAgcgc                    <  2:67135/140‑1 (MQ=255)

                                                                                                               tCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGCAACAACGGTGACTTTAGCGCCGATGGAGTCGc                                    >  1:958713/1‑123 (MQ=255)

                                                                                                               tCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGCAACAACGGTGACTTTAGCGCCGATGGAGTCGc                                    <  2:958713/123‑1 (MQ=255)

                                                                                                                  ccGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGCAACAACGGTGACTTTAGCGCCGATGGAGTCGCCCTCTTTTTTCAGcgcacgc                <  2:274165/140‑1 (MQ=255)

                                                                                                                  ccGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGCAACAACGGTGACTTTAGCGCCGATGGAGTCGCCCTCTTTTTTCAGcgcacgc                <  1:1119887/140‑1 (MQ=255)

                                                                                                                              tcatcaGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGCAACAACGGTGACTTTAGCGCCGATGGAGTCGCCCTCTTTTTTCAGCGCACGCATCAACTCGTCt    <  2:1325723/140‑1 (MQ=255)

                                                                                                                                atcaGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGCAACAACGGTGACTTTAGCGCCGATGGAGTCGCCCTCTTTTTTCAGCGCACGCATCAACTCGTCTaa  >  1:1342772/1‑140 (MQ=255)

CGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCGCATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGCAACAACGGTGACTTTAGCGCCGATGGAGTCGCCCTCTTTTTTCAGCGCACGCATCAACTCGTCTAA  >  NC_000913/2446642‑2446909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A