Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	164	66.7	2276140	99.1%	2255654	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,710,486	A→G	T95A (ACC→GCC)	rsxG →	SoxR [2Fe‑2S] reducing system protein RsxG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,710,486	0	A	G	100.0%	202.7 / NA	55	T95A (ACC→GCC)	rsxG	SoxR [2Fe‑2S] reducing system protein RsxG
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (27/28); total (27/28)

GCTGCCAGCCGAACGCTATAACAATGCGCTGGCACAGAGTTGCTATCTGGTAACTGCGCCAGAGTTAGGTAAAGGTGAGCATCGGGTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAACCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGA  >  NC_000913/1710350‑1710622

gctgcCAGCCGAACGCTATAACAATGCGCTGGCACAGAGTTGCTATCTGGTAACTGCGCCAGAGTTAGGTAAAGGTGAGCATCGGGTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCcg                                                                                                                                       >  1:505522/1‑140 (MQ=255)

     cAGCCGAACGCTATAACAATGCGCTGGCACAGAGTTGCTATCTGGTAACTGCGCCAGAGTTAGGTAAAGGTGAGCATCGGGTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCa                                                                                                                                  <  2:94583/140‑1 (MQ=255)

      aGCCGAACGCTATAACAATGCGCTGGCACAGAGTTGCTATCTGGTAACTGCGCCAGAGTTAGGTAAAGGTGAGCATCGGGTTTACATCGCCAAACAGGATGACAACCCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAg                                                                                                                                 <  1:904103/140‑1 (MQ=255)

        ccGAACGCTATAACAATGCGCTGGCACAGAGTTGCTATCTGGTAACTGCGCCAGAGTTAGGTAAAGGTGAGCATCGGGTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGAt                                                                                                                               <  1:83618/140‑1 (MQ=255)

         cGAACGCTATAACAATGCGCTGGCACAGAGTTGCTATCTGGTAACTGCGCCAGAGTTAGGTAAAGGTGAGCATCGGGTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATg                                                                                                                              >  1:668007/1‑140 (MQ=255)

           aaCGCTATAACAATGCGCTGGCACAGAGTTGCTATCTGGTAACTGCGCCAGAGTTAGGTAAAGGTGAGCATCGGGTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGc                                                                                                                            >  2:832685/1‑140 (MQ=255)

                     cAATGCGCTGGCACAGAGTTGCTATCTGGTAACTGCGCCAGAGTTAGGTAAAGGTGAGCATCGGGTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTg                                                                                                                  <  1:634308/140‑1 (MQ=255)

                         gcgcTGGCACAGAGTTGCTATCTGGTAACTGCGCCAGAGTTAGGTAAAGGTGAGCATCGGGTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGAAGCCGCCCCAGCTGGCTATTCCGGTGCAAt                                                                                                              >  2:131199/1‑140 (MQ=255)

                          cgcTGGCACAGAGTTGCTATCTGGTAACTGCGCCAGAGTTAGGTAAAGGTGAGCATCGGGTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGAtt                                                                                                             >  1:83304/1‑140 (MQ=255)

                             tGGCACAGAGTTGCTATCTGGTAACTGCGCCAGAGTTAGGTAAAGGTGAGCATCGGGTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAg                                                                                                          <  1:832685/140‑1 (MQ=255)

                                cacaGAGTTGCTATCTGGTAACTGCGCCAGAGTTAGGTAAAGGTGAGCATCGGGTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAgctg                                                                                                       >  2:969769/1‑140 (MQ=255)

                                         gCTATCTGGTAACTGCGCCAGAGTTAGGTAAAGGTGAGCATCGGGTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGa                                                                                              >  2:798043/1‑140 (MQ=255)

                                          cTATCTGGTAACTGCGCCAGAGTTAGGTAAAGGTGAGCATCGGGTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAg                                                                                             <  1:156893/140‑1 (MQ=255)

                                               tGGTAACTGCGCCAGAGTTAGGTAAAGGTGAGCATCGGGTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGAt                                                                                        >  1:540433/1‑140 (MQ=255)

                                               tGGTAACTGCGCCAGAGTTAGGTAAAGGTGAGCATCGGGTTTACATCACCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGAt                                                                                        >  2:267300/1‑140 (MQ=255)

                                                 gTAACTGCGCCAGAGTTAGGTAAAGGTGAGCATCGGGTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGAttt                                                                                      >  2:240029/1‑140 (MQ=255)

                                                    ccTGCGCCAGAGTTAGGTAAGGGTGAGTACGGGGTTTACATCGCCAAACAGGATGACAAACGGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTaa                                                                                   <  1:777059/139‑1 (MQ=255)

                                                    aCTGCGCCAGAGTTAGGTAAAGGGGAGCATCGGGTTTACATCGCCAAACAGGTTGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTaa                                                                                   <  2:658124/140‑1 (MQ=255)

                                                       gcgcCAGAGTTAGGTAAAGGTGAGCATCGGGTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGTGATTCAGCTGCTGGTGGGAGCCGATTTTAACgg                                                                                >  1:196898/1‑140 (MQ=255)

                                                       gcgcCAGAGTTAGGTAAAGGTGAGCATCGGGTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACgg                                                                                >  1:781856/1‑140 (MQ=255)

                                                         gcCAGAGTTAGGTAAAGGTGAGCATCGGGTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCa                                                                              >  1:410197/1‑140 (MQ=255)

                                                         gcCAGAGTTAGGTAAAGGTGAGCATCGGGTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCa                                                                              <  2:410197/140‑1 (MQ=255)

                                                         gcCAGAGTTAGGTAAAGGTGAGCATCGGGTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCa                                                                              <  2:83304/140‑1 (MQ=255)

                                                         gcCAGAGTTAGGTAAAGGTGAGCATCGGGTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCa                                                                              <  2:882449/140‑1 (MQ=255)

                                                         gaaaaagTAAGGAAAAGGTGGGCATGGGGTATAATTCGCAAAAAAGGCCGCCACGTGGGTGGCCGCCATTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCa                                                                              <  2:49378/135‑1 (MQ=255)

                                                                  aGGTAAAGGTGAGCATCGGGTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTg                                                                     >  2:755996/1‑140 (MQ=255)

                                                                    ggaaaGGTGAGCTTCGGGTTTATATTCCCAAAGAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGc                                                                   <  1:131199/138‑1 (MQ=255)

                                                                    gTAAAGGTGAGCATCGGGTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCTATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGc                                                                   <  2:781856/140‑1 (MQ=255)

                                                                    gTAAAGGTGAGCATCGGGTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGc                                                                   <  2:274204/140‑1 (MQ=255)

                                                                      aaaGGTGAGCAGGGGGTTTACATCGCCAAACAGGACGACAACCCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGTGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCAc                                                                 <  2:196898/140‑1 (MQ=255)

                                                                        aGGTGAGCATCGGGTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCAcgc                                                               >  1:829668/1‑140 (MQ=255)

                                                                         ggTGAGCATCGGGTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCa                                                                              <  1:178864/124‑1 (MQ=255)

                                                                         ggTGAGCATCGGGTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCa                                                                              >  2:178864/1‑124 (MQ=255)

                                                                          gcgAGCATCGGGTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCAcgcgc                                                             >  2:120909/3‑140 (MQ=255)

                                                                           tGAGCATCGGGTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCAcgcgcg                                                            <  1:240029/140‑1 (MQ=255)

                                                                           tGAGCATCGGGTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCAcgcgcg                                                            <  2:505522/140‑1 (MQ=255)

                                                                             aGCATCGGGTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTg                                                          >  1:287779/1‑140 (MQ=255)

                                                                             aGCATCGGGTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTg                                                          >  1:827437/1‑140 (MQ=255)

                                                                              gCATCGGGTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGa                                                         >  1:91907/1‑140 (MQ=255)

                                                                                 tCGGGTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACag                                                      <  1:230857/140‑1 (MQ=255)

                                                                                    ggTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGc                                                   >  2:789666/1‑140 (MQ=255)

                                                                                     gTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGca                                                  <  2:485913/140‑1 (MQ=255)

                                                                                           aTCGCAAAACAGGATGATAACCGGGTGGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCGATGCCGGGGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCTTGACAGAGCACCACGa                                            <  2:399406/140‑1 (MQ=255)

                                                                                             cGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGCGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGaaa                                          >  1:399406/1‑140 (MQ=255)

                                                                                               ccAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACg                                        >  1:730756/1‑140 (MQ=255)

                                                                                                aaaaCAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGc                                       <  2:287779/139‑1 (MQ=255)

                                                                                                        aTGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCtt                               <  1:120909/140‑1 (MQ=255)

                                                                                                        aTGACAAACCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCtt                               >  1:224939/1‑140 (MQ=255)

                                                                                                             aaaCCGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGa                          <  2:668007/140‑1 (MQ=255)

                                                                                                                 cGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATaaa                      >  2:622311/1‑140 (MQ=255)

                                                                                                                 cGGTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATaaa                      >  2:433017/1‑140 (MQ=255)

                                                                                                                   gTAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAAt                    <  1:969769/140‑1 (MQ=255)

                                                                                                                    tAGCCGCCGTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATc                   >  2:631259/1‑140 (MQ=255)

                                                                                                                       ccgccgTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGaa                <  2:540433/140‑1 (MQ=255)

                                                                                                                           cgTTCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTCGGCGATAAAATCGAACTgc            >  2:331294/1‑140 (MQ=255)

                                                                                                                              tCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGcc         >  2:673766/1‑140 (MQ=255)

                                                                                                                              tCTGGAAGCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTCGGCGATAAAATCGAACTGCGcc         <  1:331294/140‑1 (MQ=255)

                                                                                                                                     gCAGCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGa  <  1:433017/140‑1 (MQ=255)

GCTGCCAGCCGAACGCTATAACAATGCGCTGGCACAGAGTTGCTATCTGGTAACTGCGCCAGAGTTAGGTAAAGGTGAGCATCGGGTTTACATCGCCAAACAGGATGACAAACCGGTAGCCGCCGTTCTGGAAGCAACCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGA  >  NC_000913/1710350‑1710622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A