Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	164	66.7	2276140	99.1%	2255654	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,902,492	G→A	A149T (GCT→ACT)	manX →	mannose‑specific PTS enzyme IIAB component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,902,492	0	G	A	100.0%	153.2 / NA	45	A149T (GCT→ACT)	manX	mannose‑specific PTS enzyme IIAB component
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/23); total (22/23)

TGATGATGACCCAAGCTTTGATGAACTGGTGGCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGGCTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGAAACCAATGTCTCCCGTATTATT  >  NC_000913/1902362‑1902623

tgatgatgaCCCAAGCTTTGATGAACTGGTGGCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTc                                                                                                                            >  1:722007/1‑140 (MQ=255)

       gaCCCAAGCTTTGATGAACTGGTGGCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCaaa                                                                                                                     >  1:31108/1‑140 (MQ=255)

             aGCTTTGATGAACTGGTGGCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAAt                                                                                                               <  1:254703/140‑1 (MQ=255)

             aGCTTTGATGAACTGGTGGCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAAt                                                                                                               >  1:72821/1‑140 (MQ=255)

              gCTTTGATGAACTGGTGGCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATg                                                                                                              >  1:982966/1‑140 (MQ=255)

                      gaACTGGTGGCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCaaa                                                                                                      <  2:31108/140‑1 (MQ=255)

                      gaACTGGTGGCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCaaa                                                                                                      <  1:247639/140‑1 (MQ=255)

                      gaACTGGTGGCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCAGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCaaa                                                                                                      <  1:858638/140‑1 (MQ=255)

                         cTGGTGGCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGa                                                                                                   <  1:975030/140‑1 (MQ=255)

                         cTGGTGGCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGa                                                                                                   <  1:946/140‑1 (MQ=255)

                          tggtggCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGAc                                                                                                  >  2:799254/1‑140 (MQ=255)

                            gtggCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCaa                                                                                                                >  2:678454/1‑124 (MQ=255)

                            gtggCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCaa                                                                                                                <  1:678454/124‑1 (MQ=255)

                            gtggCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTa                                                                                                >  2:606995/1‑140 (MQ=255)

                            gtggCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTa                                                                                                >  1:825917/1‑140 (MQ=255)

                            gtggCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTa                                                                                                >  1:321539/1‑140 (MQ=255)

                             tggCACTGGCAGTACAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTAc                                                                                               <  2:982966/140‑1 (MQ=255)

                              ggCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACa                                                                                              <  1:771231/140‑1 (MQ=255)

                              ggCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACa                                                                                              <  1:606995/140‑1 (MQ=255)

                              ggCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACa                                                                                              <  2:72821/140‑1 (MQ=255)

                               gCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACAt                                                                                             <  2:321539/140‑1 (MQ=255)

                               gCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACAt                                                                                             >  2:473075/1‑140 (MQ=255)

                                  cTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGt                                                                                          <  1:473075/140‑1 (MQ=255)

                                   tGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACa                                                                                              <  2:1049445/135‑1 (MQ=255)

                                   tGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACa                                                                                              >  1:1049445/1‑135 (MQ=255)

                                      cAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTAtt                                                                                      >  2:319428/1‑140 (MQ=255)

                                      cAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTAtt                                                                                      >  2:290393/1‑140 (MQ=255)

                                         tAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGc                                                                                   >  2:180839/1‑140 (MQ=255)

                                               cAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTgcg                                                                             >  2:265485/1‑140 (MQ=255)

                                                aGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTgcgc                                                                            <  2:273542/140‑1 (MQ=255)

                                                aGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTgcgc                                                                            >  2:253536/1‑140 (MQ=255)

                                                 ggCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAA‑GGGGCCAAACGACTACATGGTTATTGGCCTTGCGCGt                                                                          >  2:312988/1‑140 (MQ=255)

                                                   ccGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTGCGCGTa                                                                         >  1:763633/1‑140 (MQ=255)

                                                      tGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGACAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTGCGCGTATcg                                                                      >  1:954387/1‑140 (MQ=255)

                                                      tGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAAATCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTGCGCGTATcg                                                                      >  2:355600/1‑140 (MQ=255)

                                                       gAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTGAGCGTATcga                                                                     <  2:214347/140‑1 (MQ=255)

                                                        aaGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTGCGCGTATcgac                                                                    >  1:938357/1‑140 (MQ=255)

                                                               gAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAATTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTGCGCGTATCGACGACCGTc                                                             <  2:998179/140‑1 (MQ=255)

                                                                aaaGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTGCGCGTATCGACGACCGTCt                                                            <  1:253536/140‑1 (MQ=255)

                                                                    cACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTGCGCGTATCGACGACCGTCTGAtt                                                        <  2:938357/140‑1 (MQ=255)

                                                                              cAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGAAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTGCGCGTATCGACGACCGTCTGATTCACGGTCAgg                                              <  2:574475/140‑1 (MQ=255)

                                                                                        gAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCg                                    <  1:799254/140‑1 (MQ=255)

                                                                                        gAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGACAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCg                                    <  2:954387/140‑1 (MQ=255)

                                                                                                      aGCACCCGCTGCCGCAGCACCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGaaa                      >  2:1073421/1‑140 (MQ=255)

                                                                                                                     agcaCCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGAAACCAATGTCTCCCGta       <  2:834176/140‑1 (MQ=255)

                                                                                                                        aCCAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGAAACCAATGTCTCCCGtatta    <  1:265485/140‑1 (MQ=255)

                                                                                                                          cAAAAGCGACTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGAAACCAATGTCTCCCGTattatt  <  1:149893/140‑1 (MQ=255)

TGATGATGACCCAAGCTTTGATGAACTGGTGGCACTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCACTGAAAGCCAAACCGGTTGAAAAAGCCGCGCCAGCACCCGCTGCCGCAGCACCAAAAGCGGCTCCAACTCCGGCAAAACCAATGGGGCCAAACGACTACATGGTTATTGGCCTTGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGAAACCAATGTCTCCCGTATTATT  >  NC_000913/1902362‑1902623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A