Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	164	66.7	2276140	99.1%	2255654	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,999,385	T→C	G32G (GGA→GGG)	dcyD ←	D‑cysteine desulfhydrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,999,385	0	T	C	100.0%	246.3 / NA	66	G32G (GGA→GGG)	dcyD	D‑cysteine desulfhydrase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (35/31); total (35/31)

GATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCTCCGATGTGTGTTGTTGTGATTTTCTTATTATG  >  NC_000913/1999253‑1999520

gATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGAt                                                                                                                                  >  1:513404/1‑140 (MQ=255)

 aTCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATa                                                                                                                                 >  1:493771/1‑140 (MQ=255)

  tCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATaa                                                                                                                                >  1:649356/1‑140 (MQ=255)

  tCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATaa                                                                                                                                >  2:156646/1‑140 (MQ=255)

     ccccGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCa                                                                                                                             >  1:795748/1‑140 (MQ=255)

     ccccGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCa                                                                                                                             >  2:571661/1‑140 (MQ=255)

      cccGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAg                                                                                                                            >  1:345011/1‑140 (MQ=255)

          gCAGAAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGaaaa                                                                                                                        >  2:61010/1‑140 (MQ=255)

                aTCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCgg                                                                                                                  >  1:1119258/1‑140 (MQ=255)

                aTCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCgg                                                                                                                  >  1:904418/1‑140 (MQ=255)

                      gTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGata                                                                                                            >  2:286800/1‑140 (MQ=255)

                       tATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGatat                                                                                                           >  1:777794/1‑140 (MQ=255)

                       tATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGatat                                                                                                           >  1:777793/1‑140 (MQ=255)

                        aTCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATAtt                                                                                                          >  2:551924/1‑140 (MQ=255)

                          cGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCg                                                                                                        >  2:1100153/1‑140 (MQ=255)

                          cGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCg                                                                                                        >  1:833974/1‑140 (MQ=255)

                            gCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAg                                                                                                      <  2:513404/140‑1 (MQ=255)

                               ccTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAgcgg                                                                                                   >  2:403677/1‑140 (MQ=255)

                                       cAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGgcg                                                                                           <  2:169722/140‑1 (MQ=255)

                                          agCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGgcgcgc                                                                                        >  1:631684/1‑140 (MQ=255)

                                             aTCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGa                                                                                     <  1:1100153/140‑1 (MQ=255)

                                              tCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGAt                                                                                    <  1:403677/140‑1 (MQ=255)

                                              tCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGAt                                                                                    <  2:345011/140‑1 (MQ=255)

                                                       agaAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAg                                                                           >  2:1057215/1‑140 (MQ=255)

                                                                aGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGaaa                                                                  <  2:395944/140‑1 (MQ=255)

                                                                aGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGaaa                                                                  <  2:649356/140‑1 (MQ=255)

                                                                aGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGaaa                                                                  <  1:571661/140‑1 (MQ=255)

                                                                aGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGaaa                                                                  <  2:833974/140‑1 (MQ=255)

                                                                     aCGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACggg                                                             <  2:1119258/140‑1 (MQ=255)

                                                                     aCGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACggg                                                             >  2:598983/1‑140 (MQ=255)

                                                                               ttGCCGCCCATTGACATGGGGGTGACGTCATCACATTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATg                                                   <  2:219447/140‑1 (MQ=255)

                                                                                tGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACggg                                                             >  2:517029/1‑129 (MQ=255)

                                                                                tGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACggg                                                             <  1:517029/129‑1 (MQ=255)

                                                                                tGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGc                                                  >  2:825084/1‑140 (MQ=255)

                                                                                tGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGc                                                  >  2:1104663/1‑140 (MQ=255)

                                                                                 gccgccCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCa                                                 >  2:1017466/1‑140 (MQ=255)

                                                                                           gCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAAc                                       <  1:1057215/140‑1 (MQ=255)

                                                                                           gCCATGGGGGGGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAAc                                       <  1:286800/140‑1 (MQ=255)

                                                                                                gggggTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCt                                  <  2:631684/140‑1 (MQ=255)

                                                                                                gggggTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACCCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCt                                  <  1:61010/140‑1 (MQ=255)

                                                                                                  gggTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCTcc                                >  2:340759/1‑140 (MQ=255)

                                                                                                   ggTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCTCCg                               <  2:303547/140‑1 (MQ=255)

                                                                                                   ggTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCTCCg                               <  1:156646/140‑1 (MQ=255)

                                                                                                   ggTGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCTCCg                               >  1:404624/1‑140 (MQ=255)

                                                                                                     tGACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCTCCGAt                             <  1:825084/140‑1 (MQ=255)

                                                                                                      gACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCTc                                 >  2:1125929/1‑135 (MQ=255)

                                                                                                      gACGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCTc                                 <  1:1125929/135‑1 (MQ=255)

                                                                                                       aCGTCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCTCCGAtgt                           <  2:795748/140‑1 (MQ=255)

                                                                                                          tCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCTCCGAtgtgtg                        <  1:598983/140‑1 (MQ=255)

                                                                                                          tCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCTCCGAtgtgtg                        <  1:594550/140‑1 (MQ=255)

                                                                                                          tCATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCTCCGAtgtgtg                        <  1:1017466/140‑1 (MQ=255)

                                                                                                           cATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCTCCg                               <  2:1080979/132‑1 (MQ=255)

                                                                                                           cATCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCTCCg                               >  1:1080979/1‑132 (MQ=255)

                                                                                                            aTCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCt                                  <  2:224580/128‑1 (MQ=255)

                                                                                                            aTCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCt                                  >  1:224580/1‑128 (MQ=255)

                                                                                                            aTCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCTCCGATGTGtgtt                      >  2:1128529/1‑140 (MQ=255)

                                                                                                            aTCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCTCCGATGTGtgtt                      >  2:827381/1‑140 (MQ=255)

                                                                                                            aTCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCTCCGATGTGtgtt                      <  1:1104663/140‑1 (MQ=255)

                                                                                                             tCCCGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCTCCGATGTGtgttg                     >  2:1021672/1‑140 (MQ=255)

                                                                                                              cccGTTTGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCTCCGATGTGtgttgt                    >  1:1118885/1‑140 (MQ=255)

                                                                                                                  tttGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCTCCGATGTGTGTTGTtgtg                >  2:1058083/1‑140 (MQ=255)

                                                                                                                   ttGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCTCCGATGTGTGTTGTTGTGa               <  1:1021672/140‑1 (MQ=255)

                                                                                                                   ttGATGAAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCTCCGATGTGTGTTGTTGTGa               >  1:17874/1‑140 (MQ=255)

                                                                                                                       tgaAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCTCCGATGTGTGTTGTTGTGAtttt           <  1:340759/140‑1 (MQ=255)

                                                                                                                        gaAAATTTCCCGCCCTAGATAATCAGAAAGGCGCGGCAGATATTCGAGTGGCGTTGGCGCGCAGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGTCATAACAGCCTCCGATGTGTGTTGTTGTGATTTTc          <  2:894554/140‑1 (MQ=255)

                                                                                                                        gaAAATTTCCCGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCTCCGATGTGTGTTGTTGTGATTTTc          <  2:404624/140‑1 (MQ=255)

                                                                                                                                cccGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCTCCGATGTGTGTTGTTGTGATTTTCTTATTATg  <  2:777794/140‑1 (MQ=255)

                                                                                                                                cccGCCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCTCCGATGTGTGTTGTTGTGATTTTCTTATTATg  <  2:777793/140‑1 (MQ=255)

GATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCTCCGATGTGTGTTGTTGTGATTTTCTTATTATG  >  NC_000913/1999253‑1999520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A