Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	164	66.7	2276140	99.1%	2255654	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,117,718	G→A	D229D (GAC→GAT)	wcaK ←	putative colanic acid biosynthesis pyruvyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,117,718	0	G	A	100.0%	276.7 / NA	77	D229D (GAC→GAT)	wcaK	putative colanic acid biosynthesis pyruvyl transferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (42/35); total (42/35)

CTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACGTCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATT  >  NC_000913/2117588‑2117854

cTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATccagccag                                                                                                                                 >  1:484804/1‑140 (MQ=255)

cTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATccagccag                                                                                                                                 >  2:209321/1‑140 (MQ=255)

  tCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAgtg                                                                                                                               >  2:656033/1‑140 (MQ=255)

   cATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAgtgt                                                                                                                              >  1:663069/1‑140 (MQ=255)

    aTCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGtt                                                                                                                             >  2:191429/1‑140 (MQ=255)

     tCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTg                                                                                                                            <  1:1029096/140‑1 (MQ=255)

      cGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGa                                                                                                                           >  2:392402/1‑140 (MQ=255)

       gagaATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGaa                                                                                                                          <  1:209321/140‑1 (MQ=255)

       gagaATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGaa                                                                                                                          >  2:98443/1‑140 (MQ=255)

        agaATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAAc                                                                                                                         >  1:577482/1‑140 (MQ=255)

           aTGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGc                                                                                                                      <  2:224007/140‑1 (MQ=255)

            tGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCa                                                                                                                     >  2:695849/1‑140 (MQ=255)

            tGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCa                                                                                                                     <  1:695849/140‑1 (MQ=255)

                    accaccCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGc                                                                                                             >  2:135743/1‑140 (MQ=255)

                        ccccGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTg                                                                                                         >  1:730649/1‑140 (MQ=255)

                           cGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAg                                                                                                      >  2:874532/1‑140 (MQ=255)

                            ggCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGGAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGt                                                                                                     <  1:10776/140‑1 (MQ=255)

                            ggCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGt                                                                                                     <  2:854713/140‑1 (MQ=255)

                            ggCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGt                                                                                                     <  1:98443/140‑1 (MQ=255)

                              cAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCg                                                                                                            >  1:990803/1‑131 (MQ=255)

                              cAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGtct                                                                                                   >  1:170835/1‑140 (MQ=255)

                              cAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGCCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCg                                                                                                            <  2:990803/131‑1 (MQ=255)

                               aaaGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGtctt                                                                                                  <  1:886438/140‑1 (MQ=255)

                               aaaGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGtctt                                                                                                  <  1:656033/140‑1 (MQ=255)

                               aaaGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGtctt                                                                                                  <  2:730649/140‑1 (MQ=255)

                                aaGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGtcttc                                                                                                 <  2:577482/140‑1 (MQ=255)

                                 aGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGtcttct                                                                                                <  2:484804/140‑1 (MQ=255)

                                   gCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCtgt                                                                                              >  1:372425/1‑140 (MQ=255)

                                   gCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCtgt                                                                                              >  1:1022213/1‑140 (MQ=255)

                                   gCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCtgt                                                                                              >  2:639231/1‑140 (MQ=255)

                                       tttCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTgtgg                                                                                          >  2:884949/1‑140 (MQ=255)

                                        ttCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTgtggt                                                                                         <  1:135743/140‑1 (MQ=255)

                                             aCGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGTCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCg                                                                                    >  1:1085202/1‑140 (MQ=255)

                                                  tgttgAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAg                                                                               >  1:248536/1‑140 (MQ=255)

                                                   gttgAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGc                                                                              >  1:908100/1‑140 (MQ=255)

                                                   gttgAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGc                                                                              >  2:1125186/1‑140 (MQ=255)

                                                         tggtgCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCAcgcg                                                                        >  2:377556/1‑140 (MQ=255)

                                                         tggtgCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCAcgcg                                                                        <  2:1022213/140‑1 (MQ=255)

                                                           gtgCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCTGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGt                                                                      >  2:975374/1‑140 (MQ=255)

                                                           gtgCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGCACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATAGACGAGCCACGCggg                                                                      >  2:827063/1‑139 (MQ=255)

                                                           gtgCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGt                                                                      >  2:723919/1‑140 (MQ=255)

                                                              ccGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATc                                                                   >  2:193085/1‑140 (MQ=255)

                                                              ccGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATc                                                                   >  1:11886/1‑140 (MQ=255)

                                                              ccGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGGGCGGCAACATCCCGCCAGTGTTGAACTGCATAGCTGGCGGTGAAGTCTTCTGTGTGGAGATGGATCAGCCACGCGGCATc                                                                   >  2:168788/1‑140 (MQ=255)

                                                                   aCGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGc                                                              <  1:884949/140‑1 (MQ=255)

                                                                      tttGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCAt                                                           >  2:398868/1‑140 (MQ=255)

                                                                      tttGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCAt                                                           >  2:395285/1‑140 (MQ=255)

                                                                      tttGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCAt                                                           >  2:689322/1‑140 (MQ=255)

                                                                          tCAAACGGTGCCAGTTCGCGCAGGGTAATGGTCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTc                                                       <  2:1085202/140‑1 (MQ=255)

                                                                          tCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTc                                                       <  2:170835/140‑1 (MQ=255)

                                                                          tCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTc                                                       <  1:689322/140‑1 (MQ=255)

                                                                                  tGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTg                                               >  1:590327/1‑140 (MQ=255)

                                                                                  tGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTg                                               <  2:372425/140‑1 (MQ=255)

                                                                                  tGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTg                                               <  1:1125186/140‑1 (MQ=255)

                                                                                  tGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTg                                               <  1:91935/140‑1 (MQ=255)

                                                                                   gCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGc                                              <  1:398868/140‑1 (MQ=255)

                                                                                   gCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGc                                              <  2:11886/140‑1 (MQ=255)

                                                                                      aGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCggt                                           <  1:639231/140‑1 (MQ=255)

                                                                                        ttCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCggtgg                                         <  1:605015/140‑1 (MQ=255)

                                                                                            cgcAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGat                                     >  2:826848/1‑140 (MQ=255)

                                                                                                 ggTAATGGCCACCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATtgc                                <  1:17598/140‑1 (MQ=255)

                                                                                                           aCCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCg                            >  2:304811/1‑134 (MQ=255)

                                                                                                           aCCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCg                            <  1:304811/134‑1 (MQ=255)

                                                                                                           aCCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTtcat                      >  1:1057385/1‑140 (MQ=255)

                                                                                                           aCCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTtcat                      >  1:288310/1‑140 (MQ=255)

                                                                                                           aCCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTtcat                      <  1:651452/140‑1 (MQ=255)

                                                                                                           aCCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTtcat                      >  1:633592/1‑140 (MQ=255)

                                                                                                           aCCGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTtcat                      <  1:168788/140‑1 (MQ=255)

                                                                                                             cGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTtcatca                    >  2:487177/1‑140 (MQ=255)

                                                                                                             cGTTTTCTGTTGTGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTtcatca                    >  1:869389/1‑140 (MQ=255)

                                                                                                                    tgttgtGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCaaa             <  1:395285/140‑1 (MQ=255)

                                                                                                                    tgttgtGCGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCaaa             <  1:191429/140‑1 (MQ=255)

                                                                                                                           cGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGAcc      >  2:1076574/1‑140 (MQ=255)

                                                                                                                           cGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGAcc      >  1:979543/1‑140 (MQ=255)

                                                                                                                           cGGCAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGAcc      >  1:974203/1‑140 (MQ=255)

                                                                                                                              cAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGAt   <  2:633592/140‑1 (MQ=255)

                                                                                                                              cAACATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGAt   <  2:590327/140‑1 (MQ=255)

                                                                                                                               aaCATCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGAtt  <  1:193085/140‑1 (MQ=255)

CTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACGTCCAGCCAGTGTTGAACGGCATAGCTGGCGGTGAAGTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATT  >  NC_000913/2117588‑2117854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A