Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	164	66.7	2276140	99.1%	2255654	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,181,744	G→A	A217T (GCA→ACA)	yegV →	putative sugar kinase YegV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,181,744	0	G	A	100.0%	215.8 / NA	63	A217T (GCA→ACA)	yegV	putative sugar kinase YegV
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (36/27); total (36/27)

TGACGCCGTTTATCGATTTTGGCCCACGTATTGGCGATATCCCGGATGCATTACTGGCGCGGATCATGGCCTGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCGCAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGT  >  NC_000913/2181607‑2181880

tGACGCCGTTTATCGATTTTGGCCCACGTATTGGCGATATCCCGGATGCATTACTGGCGCGGATCATGGCCTGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCcaca                                                                                                                                        >  2:182388/1‑140 (MQ=255)

 gACGCCGTTTATCGATTTTGGCCCACGTATTGGCGATATCCCGGATGCATTACTGGCGCGGATCATGGCCTGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACag                                                                                                                                       >  1:1114074/1‑140 (MQ=255)

  aCGCCGTTTATCGATTTTGGCCCACGTATTGGCGATATCCCGGATGCATTACTGGCGCGGATCATGGCCTGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACaga                                                                                                                                      <  1:1059289/140‑1 (MQ=255)

  aCGCCGTTTATCGATTTTGGCCCACGTATTGGCGATATCCCGGATGCATTACTGGCGCGGATCATGGCCTGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACaga                                                                                                                                      <  1:1123210/140‑1 (MQ=255)

  aCGCCGTTTATCGATTTTGGCCCACGTATTGGCGATATCCCGGATGCATTACTGGCGCGGATCATGGCCTGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACaga                                                                                                                                      <  2:81037/140‑1 (MQ=255)

       gTTTATCGATTTTGGCCCACGTATTGGCGATATCCCGGATGCATTGCTGGCGCGGATCATGGCCTGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATaa                                                                                                                                 >  2:781004/1‑140 (MQ=255)

       gTTTATCGATTTTGGCCCACGTATTGGCGATATCCCGGATGCATTACTGGCGCGGATCATGGCCTGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATaa                                                                                                                                 <  1:92326/140‑1 (MQ=255)

        tttATCGATTTTGGCCCACGTATTGGCGATATCCCGGATGCATTACTGGCGCGGATCATGGCCTGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATaac                                                                                                                                <  2:1114074/140‑1 (MQ=255)

        tttATCGATTTTGGCCCACGTATTGGCGATATCCCGGATGCATTACTGGCGCGGATCATGGCCTGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTCCCGCCGAACGTTTTGCTTTATCCACAGAGATaac                                                                                                                                >  2:839706/1‑140 (MQ=255)

           aTCGATTTTGGCCCACGTATTGGCGATATCCCGGATGCATTACTGGCGCGGATCATGGCCTGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATaacaac                                                                                                                             <  2:809067/140‑1 (MQ=255)

              gATTTTGGCCCACGTATTGGCGATATCCCGGATGCATTACTGGCGCGGATCATGGCCTGTCGTCCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACt                                                                                                                          <  1:839706/140‑1 (MQ=255)

              gATTTTGGCCCACGTATTGGCGATATCCCGGATGCATTACTGGCGCGGATCATGGCCTGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACt                                                                                                                          <  2:134846/140‑1 (MQ=255)

                   tGGCCCACGTATTGGCGATATCCCGGATGCATTACTGGCGCGGATCATGGCCTGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGTTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATaac                                                                                                                                >  2:846521/1‑129 (MQ=255)

                   tGGCCCACGTATTGGCGATATCCCGGATGCATTACTGGCGCGGATCATGGCCTGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGTTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATaac                                                                                                                                <  1:846521/129‑1 (MQ=255)

                                     tatCCCGGATGCATTACTGGCGCGGATCATGGCCTGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAAt                                                                                                   >  1:697835/1‑140 (MQ=255)

                                     tatCCCGGATGCATTACTGGCGCGGATCATGGCCTGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAAt                                                                                                   >  1:205646/1‑140 (MQ=255)

                                      atCCCGGATGCATTACTGGCGCGGGTCATGGCCTGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGGAAtt                                                                                                  >  2:530254/1‑140 (MQ=255)

                                        cccGGATGCATTACTGGCGCGGATCATGGCCTGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTg                                                                                                >  1:940766/1‑140 (MQ=255)

                                           ggATGCATTACTGGCGCGGATCATGGCCTGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCcg                                                                                             <  1:182388/140‑1 (MQ=255)

                                           ggATGCATTACTGGCGCGGATCATGGCCTGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCcg                                                                                             <  1:455048/140‑1 (MQ=255)

                                            gATGCATTACTGGCGCGGATCATGGCCTGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCcgc                                                                                            >  2:716313/1‑140 (MQ=255)

                                                 aTTACTGGCGCGGATCATGGCCTGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGt                                                                                       >  2:594938/1‑140 (MQ=255)

                                                 aTTACTGGCGCGGATCATGGCCTGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGt                                                                                       >  1:942903/1‑140 (MQ=255)

                                                 aTTACTGGCGCGGATCATGGCCTGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGt                                                                                       >  1:175851/1‑140 (MQ=255)

                                                   tACTGGCGCGGATCATGGCCTGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTg                                                                                     >  2:823366/1‑140 (MQ=255)

                                                          gcgGATCATGGCCTGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGAtcgttc                                                                              >  1:129503/1‑140 (MQ=255)

                                                           cgGATCATGGCCTGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCcg                                                                                             <  1:980536/124‑1 (MQ=255)

                                                           cgGATCATGGCCTGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCcg                                                                                             >  2:980536/1‑124 (MQ=255)

                                                             gATCATGGCCTGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGtc                                                                           <  1:711863/140‑1 (MQ=255)

                                                             gATCATGGCCTGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGtc                                                                           >  2:348331/1‑140 (MQ=255)

                                                                  tGGCCTGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGaa                                                                      >  2:879849/1‑139 (MQ=255)

                                                                  tGGCCTGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGAt                                                                      >  1:199484/1‑140 (MQ=255)

                                                                       tGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAaaga                                                                 <  1:815616/140‑1 (MQ=255)

                                                                       tGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAaaga                                                                 >  1:1033403/1‑140 (MQ=255)

                                                                       tGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAaaga                                                                 <  1:931990/140‑1 (MQ=255)

                                                                        gTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAaagaa                                                                >  2:746543/1‑140 (MQ=255)

                                                                        gTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAaagaa                                                                >  2:675951/1‑140 (MQ=255)

                                                                        gTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAaaga                                                                 >  2:390305/1‑139 (MQ=255)

                                                                        gTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAaaga                                                                 <  1:390305/139‑1 (MQ=255)

                                                                         tCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAaagaag                                                               >  1:562480/1‑140 (MQ=255)

                                                                         tCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAaagaag                                                               >  1:180846/1‑140 (MQ=255)

                                                                               tttAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCAt                                                         <  1:675951/140‑1 (MQ=255)

                                                                               tttAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCAt                                                         <  2:697835/140‑1 (MQ=255)

                                                                               tttAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCAt                                                         <  1:746543/140‑1 (MQ=255)

                                                                                ttAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATg                                                        >  1:695293/1‑140 (MQ=255)

                                                                                     gtCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTAtt                                                   <  1:781004/140‑1 (MQ=255)

                                                                                        gCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCa                                                >  1:119658/1‑140 (MQ=255)

                                                                                         cTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAg                                               >  1:438831/1‑140 (MQ=255)

                                                                                          tCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGc                                              <  2:119658/140‑1 (MQ=255)

                                                                                              tCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAacg                                          <  2:438831/140‑1 (MQ=255)

                                                                                               cGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAacga                                         <  1:274456/140‑1 (MQ=255)

                                                                                                 tCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAacgacg                                       >  1:273265/1‑140 (MQ=255)

                                                                                                   aaGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCt                                     >  1:510638/1‑140 (MQ=255)

                                                                                                     gagGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTc                                   >  1:333199/1‑140 (MQ=255)

                                                                                                     gagGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTc                                   <  1:192198/140‑1 (MQ=255)

                                                                                                        gCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTgg                                >  2:823846/1‑140 (MQ=255)

                                                                                                        gCTGAGATTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTgg                                <  1:716313/140‑1 (MQ=255)

                                                                                                           gagaTTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTg                             >  1:293846/1‑140 (MQ=255)

                                                                                                           gagaTTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTg                             >  1:629347/1‑140 (MQ=255)

                                                                                                            agaTTGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGc                            >  2:364525/1‑140 (MQ=255)

                                                                                                                tGCCGCCGAACGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTc                        >  1:841024/1‑140 (MQ=255)

                                                                                                                          cGTTTTGCTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTcc              <  1:654972/140‑1 (MQ=255)

                                                                                                                                 cTTTATCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCaa       >  1:4464/1‑140 (MQ=255)

                                                                                                                                     aTCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTg   >  1:163925/1‑140 (MQ=255)

                                                                                                                                      tCCACAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGt  <  2:940766/140‑1 (MQ=255)

TGACGCCGTTTATCGATTTTGGCCCACGTATTGGCGATATCCCGGATGCATTACTGGCGCGGATCATGGCCTGTCGACCTTTAGTGTCGCTCAATCGTCAAGAGGCTGAGATTGCCGCCGAACGTTTTGCTTTATCCGCAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGT  >  NC_000913/2181607‑2181880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A