Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	164	66.7	2276140	99.1%	2255654	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,211,471	G→A	A83T (GCC→ACC)	yehR →	DUF1307 domain‑containing lipoprotein YehR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,211,471	0	G	A	100.0%	171.7 / NA	50	A83T (GCC→ACC)	yehR	DUF1307 domain‑containing lipoprotein YehR
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (29/21); total (29/21)

CGGCACTGAAATTGCCATTACCTATGTCTACAAAGGTGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCGCCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTGCAGGGTATTTCAGGAATCAACGTTTC  >  NC_000913/2211335‑2211604

cGGCACTGAAATTGCCATTACCTATGTCTACAAAGGTGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCa                                                                                                                                    <  2:132464/140‑1 (MQ=255)

     cTGAAATTGCCATTACCTATGTCTACAAAGGTGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATAc                                                                                                                               >  2:474212/1‑140 (MQ=255)

             gCCATTACCTATGTCTACAAAGGTGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACAt                                                                                                                       >  1:180923/1‑140 (MQ=255)

             gCCATTACCTATGTCTACAAAGGTGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACAt                                                                                                                       >  2:199212/1‑140 (MQ=255)

             gCCATTACCTATGTCTACAAAGGTGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACAt                                                                                                                       >  1:686854/1‑140 (MQ=255)

               cATTACCTATGTCTACAAAGGTGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATcg                                                                                                                     >  2:316279/1‑140 (MQ=255)

                  tACCTATGTCTACAAAGGTGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATcgcag                                                                                                                  >  2:672357/1‑138 (MQ=255)

                           cTACAAAGGTGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTgaag                                                                                                         <  2:251621/140‑1 (MQ=255)

                            tACAAAGGTGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTgaaga                                                                                                        >  1:423254/1‑140 (MQ=255)

                              cAAAGGTGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATTGCGGGTGTTGAAGaaa                                                                                                      >  1:304768/1‑140 (MQ=255)

                              cAAAGGTGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGaaa                                                                                                      >  1:834078/1‑140 (MQ=255)

                                aaGGTGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGaaaaa                                                                                                    <  2:834078/140‑1 (MQ=255)

                                aaGGTGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGaaaaa                                                                                                    <  1:316279/140‑1 (MQ=255)

                                 aGGTGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAAt                                                                                                   <  2:631270/140‑1 (MQ=255)

                                 aGGTGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAAt                                                                                                   <  1:551596/140‑1 (MQ=255)

                                    tGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTaa                                                                                                >  2:56325/1‑140 (MQ=255)

                                    tGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTaa                                                                                                >  2:540952/1‑140 (MQ=255)

                                    tGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTaa                                                                                                >  1:233874/1‑140 (MQ=255)

                                     gACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAAc                                                                                               <  2:686854/140‑1 (MQ=255)

                                       cAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCt                                                                                             <  1:178855/140‑1 (MQ=255)

                                       cAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCt                                                                                             <  2:857069/140‑1 (MQ=255)

                                       cAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCt                                                                                             <  1:199212/140‑1 (MQ=255)

                                           gTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATAc                                                                                         <  1:791257/140‑1 (MQ=255)

                                             gCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCg                                                                                       >  1:405550/1‑140 (MQ=255)

                                             gCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCg                                                                                       >  2:288727/1‑140 (MQ=255)

                                                            tGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAgg                                                                        <  2:1113512/140‑1 (MQ=255)

                                                                   aaaaTTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGt                                                                 >  2:1067414/1‑140 (MQ=255)

                                                                   aaaaTTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGt                                                                 >  1:701344/1‑140 (MQ=255)

                                                                    aaaTTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTg                                                                <  2:423254/140‑1 (MQ=255)

                                                                     aaTTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTAcg                                                                              <  1:941836/125‑1 (MQ=255)

                                                                     aaTTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTAcg                                                                              >  2:941836/1‑125 (MQ=255)

                                                                                 cTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATgg                                                   >  1:817591/1‑140 (MQ=255)

                                                                                 cTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATgg                                                   <  2:318949/140‑1 (MQ=255)

                                                                                          tGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTgg                                          >  2:766583/1‑140 (MQ=255)

                                                                                                        aagaTGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTg                            <  1:346948/140‑1 (MQ=255)

                                                                                                         agaTGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTGc                           >  2:593598/1‑140 (MQ=255)

                                                                                                          gaTGCTGCCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTGCa                          <  1:537627/140‑1 (MQ=255)

                                                                                                             gctgcCAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTGCAggg                       <  1:288727/140‑1 (MQ=255)

                                                                                                                 ccAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTg                            >  2:358646/1‑131 (MQ=255)

                                                                                                                 ccAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTg                            <  1:358646/131‑1 (MQ=255)

                                                                                                                  cAAGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTGCAGGGTAttt                  >  1:761738/1‑140 (MQ=255)

                                                                                                                    aGACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTGCAGGGTATTTCa                >  1:547172/1‑140 (MQ=255)

                                                                                                                     gACACTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTGCAGGGTATTTCAg               >  2:363404/1‑140 (MQ=255)

                                                                                                                         cTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTGCAg                         <  1:1040304/126‑1 (MQ=255)

                                                                                                                         cTTGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTGCAg                         >  2:1040304/1‑126 (MQ=255)

                                                                                                                          ttGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTGCAGGGTATTTCAGGAATc          <  2:180923/140‑1 (MQ=255)

                                                                                                                           tGAGCCGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTGCAGGGTATTTCAGGAATCa         >  1:386506/1‑140 (MQ=255)

                                                                                                                               ccGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTGCAGGGTATTTCAGGAATCAACGt     >  2:981357/1‑140 (MQ=255)

                                                                                                                                cGTTAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTGCAGGGTATTTCAGGAATCAACGtt    >  1:931705/1‑140 (MQ=255)

                                                                                                                                  ttAAGCACCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTGCAGGGTATTTCAGGAATCAACGTTTc  >  2:1040631/1‑140 (MQ=255)

CGGCACTGAAATTGCCATTACCTATGTCTACAAAGGTGACAAGGTGCTTAAGCAATCTTCTGAAACCAAAATTCAATTTGCCTCCATTGGTGCAACCACCAAAGAAGATGCTGCCAAGACACTTGAGCCGTTAAGCGCCAAATACAAAAACATCGCGGGTGTTGAAGAAAAATTAACCTATACCGATACCTACGCGCAGGAAAACGTGACTATCGATATGGAAAAAGTGGATTTTAAAGCCCTGCAGGGTATTTCAGGAATCAACGTTTC  >  NC_000913/2211335‑2211604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A