Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	164	66.7	2276140	99.1%	2255654	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,429,397	G→A	T281M (ACG→ATG)	purF ←	amidophosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,429,397	0	G	A	100.0%	180.1 / NA	51	T281M (ACG→ATG)	purF	amidophosphoribosyltransferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (23/28); total (23/28)

AACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGCACATTGACGGGTAAACAACTGCCCTTC  >  NC_000913/2429261‑2429533

aaCGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGc                                                                                                                                       <  2:396172/140‑1 (MQ=255)

  cGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGccc                                                                                                                                     <  1:953785/140‑1 (MQ=255)

      gCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATg                                                                                                                                        >  2:97077/1‑133 (MQ=255)

       cccTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATAtt                                                                                                                                <  1:36477/140‑1 (MQ=255)

         cTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGAGCAATTTTCTCGCCCAGTTTCATGCCCATATTca                                                                                                                              <  1:137012/140‑1 (MQ=255)

           ggCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTcaca                                                                                                                            <  1:16026/140‑1 (MQ=255)

               gTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACAcgcg                                                                                                                        <  1:1004048/140‑1 (MQ=255)

                      ttGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTa                                                                                                                 >  1:381880/1‑140 (MQ=255)

                                        aTTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGAt                                                                                               >  1:393907/1‑140 (MQ=255)

                                        aTTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGAt                                                                                               >  2:626964/1‑140 (MQ=255)

                                                     tatCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCcggg                                                                                  >  2:1018796/1‑140 (MQ=255)

                                                     tatCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCcggg                                                                                  >  2:256564/1‑140 (MQ=255)

                                                        cacaCGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCcgggcgg                                                                               >  1:281140/1‑140 (MQ=255)

                                                         acacGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCcgggcggg                                                                              <  1:1018796/140‑1 (MQ=255)

                                                         acacGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCcgggcggg                                                                              <  1:869310/140‑1 (MQ=255)

                                                          cacGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCgggcgggc                                                                             <  1:256564/140‑1 (MQ=255)

                                                           acGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCa                                                                            <  2:281140/140‑1 (MQ=255)

                                                            cGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCaa                                                                           <  2:256291/140‑1 (MQ=255)

                                                             gAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCaaa                                                                          >  1:204950/1‑140 (MQ=255)

                                                              aGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAg                                                                         <  2:381880/140‑1 (MQ=255)

                                                               ggTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCcgg                                                                                   <  1:150157/129‑1 (MQ=255)

                                                               ggTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCcgg                                                                                   >  2:150157/1‑129 (MQ=255)

                                                               ggTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGt                                                                        >  2:285833/1‑140 (MQ=255)

                                                                    cTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTataca                                                                   <  2:393907/140‑1 (MQ=255)

                                                                     tGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTatacat                                                                  >  2:453435/1‑140 (MQ=255)

                                                                           cgggatcACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCaa                                                            >  2:775749/1‑140 (MQ=255)

                                                                            gggatcACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCaaa                                                           <  1:219378/140‑1 (MQ=255)

                                                                             ggatcACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAAc                                                          >  2:399044/1‑140 (MQ=255)

                                                                                 caccacGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGc                                                      >  2:937085/1‑140 (MQ=255)

                                                                                      cGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGa                                                 >  2:55502/1‑140 (MQ=255)

                                                                                      cGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGa                                                 >  2:1048722/1‑140 (MQ=255)

                                                                                        tCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGAtt                                               >  2:1014576/1‑140 (MQ=255)

                                                                                                  gATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGa                                     >  2:895873/1‑140 (MQ=255)

                                                                                                   aTCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGAt                                    >  2:809921/1‑140 (MQ=255)

                                                                                                      ttCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGt                                 <  2:757258/140‑1 (MQ=255)

                                                                                                       tCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTc                                >  2:462398/1‑140 (MQ=255)

                                                                                                               gcgGGCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGCACAtt                        <  2:380048/140‑1 (MQ=255)

                                                                                                                  ggCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGCACATTGAc                     >  1:829419/1‑140 (MQ=255)

                                                                                                                  ggCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGCACATTGAc                     >  2:224130/1‑140 (MQ=255)

                                                                                                                  ggCAATTTTCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGCACATTGAc                     <  1:1048722/140‑1 (MQ=255)

                                                                                                                       ttttCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTc                                <  1:702298/124‑1 (MQ=255)

                                                                                                                       ttttCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTc                                >  2:702298/1‑124 (MQ=255)

                                                                                                                       ttttCTCGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGCACATTGACGGGTa                <  1:55502/140‑1 (MQ=255)

                                                                                                                          tctcGCCCAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGCACATTGACGGGTAaac             <  1:1014576/140‑1 (MQ=255)

                                                                                                                               cccAGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGCACATTGACGGGTAAACAACTg        <  1:626964/140‑1 (MQ=255)

                                                                                                                                  aGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGCACATTGACGGGTAAACAACTGccc     <  2:204950/140‑1 (MQ=255)

                                                                                                                                  aGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGCACATTGACGGGTAAACAACTGccc     <  1:930467/140‑1 (MQ=255)

                                                                                                                                  aGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGCACATTGACGGGTAAACAACTGccc     <  1:399044/140‑1 (MQ=255)

                                                                                                                                  aGTTTCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGCACATTGACGGGTAAACAACTGccc     <  2:991062/140‑1 (MQ=255)

                                                                                                                                    tttCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGCACATTGACGGGTAAACAACTGCCctt   <  1:285833/140‑1 (MQ=255)

                                                                                                                                     ttCATGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGCACATTGACGGGTAAACAACTGCCcttc  <  1:809921/140‑1 (MQ=255)

AACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGCACATTGACGGGTAAACAACTGCCCTTC  >  NC_000913/2429261‑2429533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A