Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	164	66.7	2276140	99.1%	2255654	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,656,054	T→C	N102S (AAC→AGC)	pepB ←	aminopeptidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,656,054	0	T	C	100.0%	219.5 / NA	59	N102S (AAC→AGC)	pepB	aminopeptidase B
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (33/26); total (33/26)

ATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACAT  >  NC_000913/2655927‑2656186

aTAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCAAGTTCCt                                                                                                                          >  1:294341/1‑140 (MQ=255)

          cGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGc                                                                                                                >  1:519878/1‑140 (MQ=255)

           gATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCa                                                                                                               <  2:356661/140‑1 (MQ=255)

              cGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCa                                                                                                               >  1:507749/1‑137 (MQ=255)

              cGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCa                                                                                                               <  2:507749/137‑1 (MQ=255)

              cGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCg                                                                                                            >  2:1029630/1‑140 (MQ=255)

               gCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGt                                                                                                           >  1:98445/1‑140 (MQ=255)

                       cGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATcc                                                                                                   >  1:26454/1‑140 (MQ=255)

                                  agatcaACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCAcaccac                                                                                        >  2:156753/1‑140 (MQ=255)

                                    atcaACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACtt                                                                                      >  1:92238/1‑140 (MQ=255)

                                         cAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCgg                                                                                                 <  2:177345/124‑1 (MQ=255)

                                         cAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCgg                                                                                                 >  1:177345/1‑124 (MQ=255)

                                         cAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCcaca                                                                                            <  1:186639/129‑1 (MQ=255)

                                         cAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCcaca                                                                                            >  2:186639/1‑129 (MQ=255)

                                         cAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTAcgc                                                                                 >  2:884848/1‑140 (MQ=255)

                                         cAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTAcgc                                                                                 >  1:499158/1‑140 (MQ=255)

                                           gCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGt                                                                               >  2:51304/1‑140 (MQ=255)

                                            cACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTg                                                                              >  2:36596/1‑140 (MQ=255)

                                              cGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGcc                                                                            >  1:736517/1‑140 (MQ=255)

                                              cGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGcc                                                                            >  1:397424/1‑140 (MQ=255)

                                                   tgCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCttt                                                                         <  1:470061/138‑1 (MQ=255)

                                                   tgCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCttt                                                                         >  2:470061/1‑138 (MQ=255)

                                                    gCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCgg                                                                      <  2:229164/140‑1 (MQ=255)

                                                    gCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCgg                                                                      <  1:264253/140‑1 (MQ=255)

                                                    gCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCgg                                                                      >  1:661510/1‑140 (MQ=255)

                                                     ccAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCggg                                                                     <  1:364943/140‑1 (MQ=255)

                                                     ccAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCAAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCggg                                                                     <  2:294341/140‑1 (MQ=255)

                                                           gCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTg                                                               >  1:228387/1‑140 (MQ=255)

                                                            cGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGt                                                              <  2:1010004/140‑1 (MQ=255)

                                                              aTGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTaa                                                            <  1:884848/140‑1 (MQ=255)

                                                                ggTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAAcc                                                          >  1:12991/1‑140 (MQ=255)

                                                                    ccAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGc                                                      >  1:391820/1‑140 (MQ=255)

                                                                        ttcttcTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGa                                                  <  1:725225/140‑1 (MQ=255)

                                                                        ttcttcTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGa                                                  <  1:221083/140‑1 (MQ=255)

                                                                        ttcttcTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCAGGGCTTTGTAACCTTGCCAGa                                                  <  1:744218/140‑1 (MQ=255)

                                                                            tctGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGc                                              >  2:111878/1‑140 (MQ=255)

                                                                                ccGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCcag                                          >  2:860721/1‑140 (MQ=255)

                                                                                  ggTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCcagca                                        >  1:482520/1‑140 (MQ=255)

                                                                                   gTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCcagcag                                       <  1:581393/140‑1 (MQ=255)

                                                                                    tGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCagcagc                                      <  2:12991/140‑1 (MQ=255)

                                                                                     gCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCg                                     <  2:26454/140‑1 (MQ=255)

                                                                                              gtgtCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCg                                <  1:1107692/136‑1 (MQ=255)

                                                                                              gtgtCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCg                                >  2:1107692/1‑136 (MQ=255)

                                                                                              gtgtCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATc                            <  1:649885/140‑1 (MQ=255)

                                                                                                gtCACGCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATccc                          >  1:588938/1‑140 (MQ=255)

                                                                                                     gCACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCt                     >  1:287412/1‑140 (MQ=255)

                                                                                                      cACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCtt                    >  2:1048761/1‑140 (MQ=255)

                                                                                                      cACCCAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCtt                    <  2:397424/140‑1 (MQ=255)

                                                                                                       ccccAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTc                   <  2:391820/139‑1 (MQ=255)

                                                                                                         ccAGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCAc                 >  2:678536/1‑140 (MQ=255)

                                                                                                           aGTCGATGATCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCAGCCCAGCCTTCACCg               >  2:697039/1‑140 (MQ=255)

                                                                                                                atgatCATCAGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTaa          <  1:1129582/140‑1 (MQ=255)

                                                                                                                       tcaGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCg               >  1:551021/1‑128 (MQ=255)

                                                                                                                       tcaGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCg               <  2:551021/128‑1 (MQ=255)

                                                                                                                       tcaGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACa   >  1:348392/1‑140 (MQ=255)

                                                                                                                       tcaGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACa   >  1:711099/1‑140 (MQ=255)

                                                                                                                       tcaGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACa   <  1:156753/140‑1 (MQ=255)

                                                                                                                       tcaGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACa   <  2:92238/140‑1 (MQ=255)

                                                                                                                       tcaGGCCGCTATCTAGTTCCTGGCGCTGGGCATCGTCGAGATGCGGCCACACCACTTTACGCGTGCCTGTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATTTGCATCGCAGCCTTCACCGCTTAACTGATCa   >  2:993530/1‑140 (MQ=255)

                                                                                                                        caGGCGGCTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACAt  <  1:1010938/140‑1 (MQ=255)

ATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACAT  >  NC_000913/2655927‑2656186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A