Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	164	66.7	2276140	99.1%	2255654	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,717,568	C→T	G321S (GGC→AGC)	yfiF ←	putative methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,717,568	0	C	T	100.0%	225.5 / NA	64	G321S (GGC→AGC)	yfiF	putative methyltransferase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (31/33); total (31/33)

AGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCCCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTAACCCTTCATATTCCTGACCCAGCACCAGTACCATTTTCGCTGGCAGACTAGTTTTGAACAGCGGTTTACCCTGCTCGCTGGA  >  NC_000913/2717431‑2717703

agagagTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCg                                                                                                                                       >  1:706850/1‑140 (MQ=255)

 gagagTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGt                                                                                                                                      >  2:909/1‑140 (MQ=255)

 gagagTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGt                                                                                                                                      <  1:549280/140‑1 (MQ=255)

  agagTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTa                                                                                                                                     >  2:95170/1‑140 (MQ=255)

  agagTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTa                                                                                                                                     >  2:770006/1‑140 (MQ=255)

  agagTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTa                                                                                                                                     <  1:421485/140‑1 (MQ=255)

    agtgagtAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTAcc                                                                                                                                   >  2:187687/1‑140 (MQ=255)

        agtAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCa                                                                                                                               >  1:675411/1‑140 (MQ=255)

         gtAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCg                                                                                                                                       >  1:206897/1‑131 (MQ=255)

             aTTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAATGTTGCTCGTACCGTCAATCtt                                                                                                                          <  2:504033/140‑1 (MQ=255)

             aTTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCtt                                                                                                                          <  2:335669/140‑1 (MQ=255)

             aTTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCtt                                                                                                                          <  2:875564/140‑1 (MQ=255)

             aTTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCtt                                                                                                                          <  2:569254/140‑1 (MQ=255)

             aTTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCtt                                                                                                                          <  1:127326/140‑1 (MQ=255)

              tttGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTc                                                                                                                         >  1:732514/1‑140 (MQ=255)

                  cGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCAcgc                                                                                                                     >  1:645211/1‑140 (MQ=255)

                       aaaTGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGTCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGa                                                                                                                <  1:452055/140‑1 (MQ=255)

                        aaTGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGAt                                                                                                               <  2:732514/140‑1 (MQ=255)

                                  ggCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATc                                                                                                     >  2:720490/1‑140 (MQ=255)

                                    cAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCa                                                                                                                  <  2:388413/125‑1 (MQ=255)

                                    cAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCa                                                                                                                  >  1:388413/1‑125 (MQ=255)

                                       agTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTg                                                                                                <  1:720490/140‑1 (MQ=255)

                                           gCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGc                                                                                            >  1:812508/1‑140 (MQ=255)

                                            ccACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCa                                                                                           <  1:911063/140‑1 (MQ=255)

                                            ccACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCa                                                                                           <  1:1085600/140‑1 (MQ=255)

                                             cACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCAt                                                                                          >  2:755322/1‑140 (MQ=255)

                                                  ggCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGt                                                                                     <  2:683846/140‑1 (MQ=255)

                                                  ggCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGt                                                                                     <  1:2670/140‑1 (MQ=255)

                                                   gCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTa                                                                                    <  2:675551/140‑1 (MQ=255)

                                                   gCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTa                                                                                    >  1:963759/1‑140 (MQ=255)

                                                    cTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTaa                                                                                   >  1:824026/1‑140 (MQ=255)

                                                      gATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTAAcc                                                                                 <  1:1023524/140‑1 (MQ=255)

                                                         ttATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTAACCCtt                                                                              >  2:1076464/1‑140 (MQ=255)

                                                           aTCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTAACCCTTCa                                                                            <  1:846045/140‑1 (MQ=255)

                                                                 cTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTaa                                                                                   >  2:194465/1‑127 (MQ=255)

                                                                 cTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTaa                                                                                   <  1:194465/127‑1 (MQ=255)

                                                                 cTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTAACCCTTCATATTcc                                                                      <  2:824026/140‑1 (MQ=255)

                                                                    tATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTAACCCTTCATATTCCTGa                                                                   <  1:1044359/140‑1 (MQ=255)

                                                                         tGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTAACCCTTCATATTCCTGACCCAg                                                              <  1:909/140‑1 (MQ=255)

                                                                           aCGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTAACCCTTCATATTCCTGACCCAGCa                                                            >  1:764014/1‑140 (MQ=255)

                                                                            cGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTAACCCTTCATATTCCTGACCCAGCAc                                                           <  1:755322/140‑1 (MQ=255)

                                                                                accaTTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTAACCCTTCATATTCCTGACCCAGCACCAGt                                                       >  1:593026/1‑140 (MQ=255)

                                                                                accaTTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGCAACCCTTCATATTCCTGACCCAGCACCAGt                                                       >  1:688990/1‑140 (MQ=255)

                                                                                     tCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTAACCCTTCATATTCCTGACCCAGCACCAGTACCAt                                                  >  2:116427/1‑140 (MQ=255)

                                                                                           aGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGTTAACCCTTCATATTCCTGACCCAGCACCAGTACCATTTTCGc                                            >  2:1060044/1‑140 (MQ=255)

                                                                                           aGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTAACCCTTCATATTCCTGACCCAGCACCAGTACCATTTTCGc                                            >  2:795309/1‑140 (MQ=255)

                                                                                            gCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTAACCCTTCATATTCCTGACCCAGCACCAGTACCATTTTCGCt                                           >  1:460005/1‑140 (MQ=255)

                                                                                                 aCGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTAACCCTTCATATTCCTGACCCAGCACCAGTACCATTTTCGCTGGCAg                                      >  2:885985/1‑140 (MQ=255)

                                                                                                               agagATATTCAGGCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTAACCCTTCATATTCCTGACCCAGCACCAGTACCATTTTCGCTGGCAGACTAGTTTTGAACa                        <  2:496403/140‑1 (MQ=255)

                                                                                                               agagATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTAACCCTTCATATTCCTGACCCAGCACCAGTACCATTTTCGCTGGCAGACTAGTTTTGAACa                        <  2:645211/140‑1 (MQ=255)

                                                                                                               agagATATTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTAACCCTTCATATTCCTGACCCAGCACCAGTACCATTTTCGCTGGCAGACTAGTTTTGAACa                        <  2:277995/140‑1 (MQ=255)

                                                                                                                  gaTATTCAGCCCGGCAACGTTGCTCGCACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTAACCCTTCATATTCCTGACCCAGCACCAGTACCATTTTCGCTGGCAGACTAGTTTTGAACAGCg                     <  1:795309/140‑1 (MQ=255)

                                                                                                                    tatTCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTAACCCTTCATATTCCTGACCCAGCACCAGTACCATTTTCGCTGGCAGACTAGTTTTGAACAGCGGt                   <  1:885985/140‑1 (MQ=255)

                                                                                                                      ttCAGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTAACCCTTCATATTCCTGACCCAGCACCAGTACCATTTTCGCTGGCAGACTAGTTTTGAACAGCGGttt                 <  2:812508/140‑1 (MQ=255)

                                                                                                                         aGCCCGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTAACCCTTCATATTCCTGACCCAGCACCAGTACCATTTTCGCTGGCAGACTAGTTTTGAACAGCGGTTTAcc              >  1:953071/1‑140 (MQ=255)

                                                                                                                           cccGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTAACCCTTCATATTCCTGACCCAGCACCAGTACCATTTTCGCTGGCAGACTAGTTTTGAACAGCGGTTTACCCt            >  1:908129/1‑140 (MQ=255)

                                                                                                                            ccGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTAACCCTTCATATTCCTGACCCAGCACCAGTACCATTTTCGCTGGCAGACTAGTTTTGAACAGCGGTTTACCCTg           <  1:1060044/140‑1 (MQ=255)

                                                                                                                            ccGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTAACCCTTCATATTCCTGACCCAGCACCAGTACCATTTTCGCTGGCAGACTAGTTTTGAACAGCGGTTTACCCTg           <  2:764014/140‑1 (MQ=255)

                                                                                                                            ccGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTAACCCTTCATATTCCTGACCCAGCACCAGTACCATTTTCGCTGGCAGACTAGTTTTGAACAGCGGTTTACCCTg           <  1:750033/140‑1 (MQ=255)

                                                                                                                            ccGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTAACCCTTCATATTCCTGACCCAGCACCAGTACCATTTTCGCTGGCAGACTAGTTTTGAACAGCGGTTTACCCTg           <  1:770006/140‑1 (MQ=255)

                                                                                                                             cGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTAACCCTTCATATTCCTGACCCAGCACCAGTACCATTTTCGCTGGCAGACTAGTTTTGAACa                        >  1:656234/1‑126 (MQ=255)

                                                                                                                             cGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTAACCCTTCATATTCCTGACCCAGCACCAGTACCATTTTCGCTGGCAGACTAGTTTTGAACa                        <  2:656234/126‑1 (MQ=255)

                                                                                                                             cGGCAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTAACCCTTCATATTCCTGACCCAGCACCAGTACCATTTTCGCTGGCAGACTAGTTTTGAACAGCGGTTTACCCTGc          >  1:1950/1‑140 (MQ=255)

                                                                                                                                cAACGTTGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTAACCCTTCATATTCCTGACCCAGCACCAGTACCATTTTCGCTGGCAGACTAGTTTTGAACAGCGGTTTACCCTGCTCg       >  1:676951/1‑140 (MQ=255)

                                                                                                                                     ttGCTCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTAACCCTTCATATTCCTGACCCAGCACCAGTACGATTTTCGCTGGCAGACTAGTTTTGAACAGCGGTTTACCCTGCTCGCTGGa  >  2:1017434/1‑140 (MQ=255)

AGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACGCCGGTTGCGACAGAGATATTCAGCCCGGCAACGTTGCCCGTACCGTCAATCTTCACGCGCAGATCGTTCGGATCGCGTGCGGCATCCGGTAACCCTTCATATTCCTGACCCAGCACCAGTACCATTTTCGCTGGCAGACTAGTTTTGAACAGCGGTTTACCCTGCTCGCTGGA  >  NC_000913/2717431‑2717703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A