Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F28 I1 R1 164 66.7 2276140 99.1% 2255654 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,196,990 T→C E884E (GAA→GAG)  glnE ← fused glutamine synthetase deadenylase/glutamine synthetase adenylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,196,9900TC100.0% 230.5 / NA 61E884E (GAA→GAG) glnEfused glutamine synthetase deadenylase/glutamine synthetase adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/37);  total (24/37)

CGGTGAAGCAATCCTCCGACACATGGCCCGGCAATTCCTGTAATGCCAGATGATGAAGTTCATCGCGAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGTTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCAACACCAGATATTGGGTAATAAATTCGATATCGGTAATTCCCCCTTCATCAGCTTTGATATCAAAGCGATCGCGA  >  NC_000913/3196854‑3197125
                                                                                                                                        |                                                                                                                                       
cGGTGAAGCAATCCTCCGACACATGGCCCGGCAATTCCTGTAATGCCAGATGATGAAGTTCATCGCGAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTcc                                                                                                                                      <  2:191906/140‑1 (MQ=255)
    gAAGCAATCCTCCGACACATGGCCCGGCAATTCCTGTAATGCCAGATGATGAAGTTCATCGCGAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGaa                                                                                                                                  <  1:462418/140‑1 (MQ=255)
      aGCAATCCTCCGACACATGGCCCGGCAATTCCTGTAATGCCAGATGATGAAGTTCATCGCGGAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATa                                                                                                                                >  2:1014808/1‑140 (MQ=255)
        cAATCCTCCGACACATGGCCCGGCAATTCCTGTAATGCCAGATGATGAAGTTCATCGCGAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACg                                                                                                                              >  1:341172/1‑140 (MQ=255)
          aTCCTCCGACACATGGCCCGGCAGTTCCTGTAATGCCAGATGATGAAGTTCATCGCGAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCa                                                                                                                            >  1:1117735/1‑140 (MQ=255)
          aTCCTCCGACACATGGCCCGGCAATTCCTGTAATGCCAGATGATGAAGTTCATCGCGAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCa                                                                                                                            <  2:145614/140‑1 (MQ=255)
           tcctccGACACATGGCCCGGCAATTCCTGTAATGCCAGATGATGAAGTTCATCGCGAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCAc                                                                                                                           <  1:1014808/140‑1 (MQ=255)
           tcctccGACACATGGCCCGGCAATTCCTGTAATGCCAGATGATGAAGTTCATCGCGAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCAc                                                                                                                           <  2:888747/140‑1 (MQ=255)
           tcctccGACACATGGCCCGGCAATTCCTGTAATGCCAGATGATGAAGTTCATCGCGAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCACCAGTAGCTCCAGAATACGCAc                                                                                                                           <  1:922589/140‑1 (MQ=255)
                 gACACATGGCCCGGCAATTCCTGTAATGCCAGATGATGCAGTTCATCGCGAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTc                                                                                                                     <  2:952171/140‑1 (MQ=255)
                        ggCCCGGCAATTCCTGTAATGCCAGATGATGAAGTTCATCGCGAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAg                                                                                                              >  2:539828/1‑140 (MQ=255)
                        ggCCCGGCAATTCCTGTAATGCCAGATGATGAAGTTCATCGCGAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAg                                                                                                              <  1:539828/140‑1 (MQ=255)
                          cccGGCAATTCCTGTAATGCCAGATGATGAAGTTCATCGCGAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAgcg                                                                                                            >  2:207552/1‑140 (MQ=255)
                             ggCAATTCCTGTAATGCCAGATGATGAAGTTCATCGCGAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCa                                                                                                               <  2:149054/134‑1 (MQ=255)
                             ggCAATTCCTGTAATGCCAGATGATGAAGTTCATCGCGAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCa                                                                                                               >  1:149054/1‑134 (MQ=255)
                              gCAATTCCTGTAATGCCAGATGATGAAGTTCATCGCGAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAg                                                                                                              <  1:801810/134‑1 (MQ=255)
                              gCAATTCCTGTAATGCCAGATGATGAAGTTCATCGCGAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAg                                                                                                              >  2:801810/1‑134 (MQ=255)
                                         aaTGCCAGATGATGAAGTTCATCGCGAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGttt                                                                                             <  2:926658/140‑1 (MQ=255)
                                           tGCCAGATGATGAAGTTCATCGCGAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGttttt                                                                                           >  2:895691/1‑140 (MQ=255)
                                             ccAGATGATGAAGTTCATCGCGAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCa                                                                                         >  2:758109/1‑140 (MQ=255)
                                             ccAGATGATGAAGTTCATCGCGAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCa                                                                                         >  2:1067315/1‑140 (MQ=255)
                                                     tgaAGTTCATCGCGAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTa                                                                                 >  1:604648/1‑140 (MQ=255)
                                                        aGTTCATCGCGAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAgcg                                                                              <  1:640069/140‑1 (MQ=255)
                                                        aGTTCATCGCGAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAgcg                                                                              <  2:38329/140‑1 (MQ=255)
                                                        aGTTCATCGCGAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAgcg                                                                              <  1:207552/140‑1 (MQ=255)
                                                         gTTCATCGCGAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAgcgc                                                                             <  2:341172/140‑1 (MQ=255)
                                                          ttCATCGCGAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCa                                                                            <  2:395036/140‑1 (MQ=255)
                                                          ttCATCGCGAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCa                                                                            <  2:483013/140‑1 (MQ=255)
                                                          ttCATCGCGAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCa                                                                            <  2:1117735/140‑1 (MQ=255)
                                                          ttCATCGCGAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCa                                                                            <  1:840862/140‑1 (MQ=255)
                                                            cATCGCGAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCAac                                                                          >  2:75877/1‑140 (MQ=255)
                                                              tCGCGAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCAacac                                                                        >  1:112759/1‑140 (MQ=255)
                                                               cgcgAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCAACAcc                                                                       <  1:231985/140‑1 (MQ=255)
                                                                 cgAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAgcg                                                                              >  1:995171/1‑131 (MQ=255)
                                                                 cgAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAgcg                                                                              <  2:995171/131‑1 (MQ=255)
                                                                    aGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCAACACCAGata                                                                  <  1:895691/140‑1 (MQ=255)
                                                                    aGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCAACACCAGata                                                                  <  2:241817/140‑1 (MQ=255)
                                                                            gtAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCAACACCAGATATTGGGtaa                                                          <  1:95539/140‑1 (MQ=255)
                                                                             tAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCAACACCAGATATTGGGtaat                                                         <  2:604648/140‑1 (MQ=255)
                                                                                gCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATCCGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCAACACCAGATAtt                                                                <  2:1056795/130‑1 (MQ=255)
                                                                                gCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCAACACCAGATAtt                                                                >  1:1056795/1‑130 (MQ=255)
                                                                                gCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCAACACCAGATATTGGGTAATaaa                                                      >  2:636742/1‑140 (MQ=255)
                                                                                     ggTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCAACACCAGATATTgg                                                              >  2:684543/1‑127 (MQ=255)
                                                                                     ggTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCAACACCAGATATTgg                                                              <  1:684543/127‑1 (MQ=255)
                                                                                     ggTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCAACACCAGATATTGGGTAATAAATTCGa                                                 >  2:995804/1‑140 (MQ=255)
                                                                                     ggTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCAACACCAGATATTGGGTAATAAATTCGa                                                 >  1:1054365/1‑140 (MQ=255)
                                                                                     ggTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCAACACCAGATATTGGGTAATAAATTCGa                                                 >  2:24569/1‑140 (MQ=255)
                                                                                      gTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCAACACCAGATATTGGGTAATAAATTCGat                                                <  2:982426/140‑1 (MQ=255)
                                                                                                tCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCAACACCAGATATTGGGTAATAAATTCGATATCGGTAAtt                                      >  1:433619/1‑140 (MQ=255)
                                                                                                tCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCAACACCAGATATTGGGTAATAAATTCGATATCGGTAAtt                                      <  1:1074979/140‑1 (MQ=255)
                                                                                                tCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCAACACCAGATATTGGGTAATAAATTCGATATCGGTAAtt                                      >  2:228030/1‑140 (MQ=255)
                                                                                                     tCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCAACACCAGATATTGGGTAATAAATTCGATATCGGTAATTccccc                                 <  1:758109/140‑1 (MQ=255)
                                                                                                     tCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCAACACCAGATATTGGGTAATAAATTCGATATCGGTAATTccccc                                 <  1:1067315/140‑1 (MQ=255)
                                                                                                          ctctTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCAACACCAGATATTGGGTAATAAATTCGATATCGGTAATTCCCCCTtcat                            <  1:228030/140‑1 (MQ=255)
                                                                                                          ctctTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCAACACCAGATATTGGGTAATAAATTCGATATCGGTAATTCCCCCTtcat                            <  1:118121/140‑1 (MQ=255)
                                                                                                           tctTCCATAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCAACACCAGATATTGGGTAATAAATTCGATATCGGTAATTCCCCCTtcatc                           <  2:112759/140‑1 (MQ=255)
                                                                                                                  tAATGTCGTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCAACACCAGATATTGGGTAATAAATTCGATATCGGTAATTCCCCCTTCATCAGCTTTg                    <  2:934857/140‑1 (MQ=255)
                                                                                                                         gTTTTGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCAACACCAGATATTGGGTAATAAATTCGATATCGGTAATTCCCCCTTCATCAGCTTTGATATCaa             >  2:471588/1‑140 (MQ=255)
                                                                                                                            ttGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCAACACCAGATATTGGGTAATAAATTCGATATCGGTAATTCCCCCTTCATCAGCTTTGATATCaaa            >  1:635822/1‑138 (MQ=255)
                                                                                                                            ttGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCAACACCAGATATTGGGTAATAAATTCGATATCGGTAATTCCCCCTTCATCAGCTTTGATATCaaa            <  2:635822/138‑1 (MQ=255)
                                                                                                                            ttGCGCCAGTAGCTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCAACACCAGATATTGGGTAATAAATTCGATATCGGTAATTCCCCCTTCATCAGCTTTGATATCAAAGc          <  1:24569/140‑1 (MQ=255)
                                                                                                                                   agtagcTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCAACACCAGATATTGGGTAATAAATTCGATATCGGTAATTCCCCCTTCATCAGCTTTGATATCAAAGCGATcgcg   <  2:1016333/140‑1 (MQ=255)
                                                                                                                                    gtagcTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCAACACCAGATATTGGGTAATAAATTCGATATCGGTAATTCCCCCTTCATCAGCTTTGATATCAAAGCGATCGCGa  >  2:264673/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                       
CGGTGAAGCAATCCTCCGACACATGGCCCGGCAATTCCTGTAATGCCAGATGATGAAGTTCATCGCGAAGCGTAGTGTAAGCACGGGTCAGCGCCATCGCTTCCTGCTCTTCCATAATGTCGTTTTGCGCCAGTAGTTCCAGAATACGCACGTTGTCTGACCAGCGCGTTAACTTCGGTTTTTCATGAGCGTAGCGCAACACCAGATATTGGGTAATAAATTCGATATCGGTAATTCCCCCTTCATCAGCTTTGATATCAAAGCGATCGCGA  >  NC_000913/3196854‑3197125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A