Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	164	66.7	2276140	99.1%	2255654	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,201,912	G→A	G8S (GGT→AGT)	cca →	fused tRNA nucleotidyltransferase/2',3'‑cyclic phosphodiesterase/2' nucleotidase and phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,201,912	0	G	A	100.0%	180.6 / NA	52	G8S (GGT→AGT)	cca	fused tRNA nucleotidyltransferase/2',3'‑cyclic phosphodiesterase/2' nucleotidase and phosphatase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (34/18); total (34/18)

CCGCACCTGATCCCAAGCCGCAAACGCAAAGATCGCTGGATGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCA  >  NC_000913/3201780‑3202048

ccGCACCTGATCCCAAGCCGCAAACGCAAAGATCGCTGGATGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGt                                                                                                                                   >  2:485252/1‑140 (MQ=255)

ccGCACCTGATCCCAAGCCGCAAACGCAAAGATCGCTGGATGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGt                                                                                                                                   >  2:662657/1‑140 (MQ=255)

        gATCCCAAGCCGCAAACGCAAAGATCGCTGGATGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATg                                                                                                                           <  2:680958/140‑1 (MQ=255)

          tCCCAAGCCGCAAACGCAAAGATCGCTGGATGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCa                                                                                                                         <  1:550113/140‑1 (MQ=255)

                   gcaaacgcaaaGATCGCTGGATGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTTTCTGGTCGGTAGTGCTGTTCGGGATGc                                                                                                                          <  2:535559/130‑1 (MQ=255)

                   gcaaacgcaaaGATCGCTGGATGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGc                                                                                                                          >  1:535559/1‑130 (MQ=255)

                   gcaaacgcaaaGATCGCTGGATGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAggg                                                                                                                <  1:605464/140‑1 (MQ=255)

                    caaacgcaaaGATCGCTGGATGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCggg                                                                                                                              <  2:82501/125‑1 (MQ=255)

                    caaacgcaaaGATCGCTGGATGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCggg                                                                                                                              >  1:82501/1‑125 (MQ=255)

                             aGATCGCTGGATGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCa                                                                                                      <  2:707031/140‑1 (MQ=255)

                             aGATCGCTGGATGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCa                                                                                                      <  1:662657/140‑1 (MQ=255)

                                   cTGGATGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACa                                                                                                >  2:305952/1‑140 (MQ=255)

                                                aTCGCCTTCTTCGTTGCACTGTCTTATTATTGGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGggtggt                                                                                   >  1:241315/1‑140 (MQ=255)

                                                aTCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGAATTTGACGAAGGGATGATGGCGTGAAGATTTGTCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGggtggt                                                                                   >  2:699618/1‑140 (MQ=255)

                                                           cGTTGCACTGTCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTa                                                                        <  1:563902/140‑1 (MQ=255)

                                                              tGCACTGTCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGc                                                                     >  2:1012490/1‑140 (MQ=255)

                                                                     tCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGga                                                              >  2:253484/1‑140 (MQ=255)

                                                                     tCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGga                                                              <  2:970841/140‑1 (MQ=255)

                                                                     tCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGga                                                              >  1:562588/1‑140 (MQ=255)

                                                                     tCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGga                                                              >  2:867390/1‑140 (MQ=255)

                                                                     tCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGAAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGga                                                              >  2:1014427/1‑140 (MQ=255)

                                                                               aGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGAc                                                    >  2:480998/1‑140 (MQ=255)

                                                                               aGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGAc                                                    >  1:786439/1‑140 (MQ=255)

                                                                               aGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGAc                                                    >  1:258287/1‑140 (MQ=255)

                                                                               aGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGAc                                                    >  1:143136/1‑140 (MQ=255)

                                                                               aGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGAc                                                    >  2:45117/1‑140 (MQ=255)

                                                                               aGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGAc                                                    >  2:446829/1‑140 (MQ=255)

                                                                               aGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGAc                                                    >  2:244575/1‑140 (MQ=255)

                                                                                ggAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGAcg                                                   <  1:305952/140‑1 (MQ=255)

                                                                                   aTTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCgg                                                >  1:314320/1‑140 (MQ=255)

                                                                                     tgtgTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGc                                              >  2:452425/1‑140 (MQ=255)

                                                                                     tgtgTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGc                                              >  1:1124203/1‑140 (MQ=255)

                                                                                     tgtgTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGc                                              <  1:1012490/140‑1 (MQ=255)

                                                                                     tgtgTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGc                                              <  1:867390/140‑1 (MQ=255)

                                                                                      gtgtATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCt                                             <  2:385818/140‑1 (MQ=255)

                                                                                       tgtATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTa                                            >  1:646484/1‑140 (MQ=255)

                                                                                       tgtATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTa                                            >  1:239138/1‑140 (MQ=255)

                                                                                          aTTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACca                                         <  2:241315/140‑1 (MQ=255)

                                                                                            ttGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACcagc                                       >  2:445620/1‑140 (MQ=255)

                                                                                            ttGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACcagc                                       >  2:111064/1‑140 (MQ=255)

                                                                                               aCGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAgg                                    >  2:520083/1‑140 (MQ=255)

                                                                                                cGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGt                                   <  1:485252/140‑1 (MQ=255)

                                                                                                   aggaatgatgGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAgg                                >  2:1062288/5‑140 (MQ=255)

                                                                                                   aGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAgg                                >  2:1069460/1‑140 (MQ=255)

                                                                                                   aGGGATGATGGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAgg                                >  1:133811/1‑140 (MQ=255)

                                                                                                        tgatgGCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCcgcg                           <  2:562588/140‑1 (MQ=255)

                                                                                                             gCGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGAtttt                      >  2:313080/1‑140 (MQ=255)

                                                                                                              cGTGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTc                     >  2:559261/1‑140 (MQ=255)

                                                                                                                tGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCt                   <  1:253484/140‑1 (MQ=255)

                                                                                                                tGAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCt                   >  1:124846/1‑140 (MQ=255)

                                                                                                                 gAAGATTTATCTGGTCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCtg                  >  1:475217/1‑140 (MQ=255)

                                                                                                                               tCGGTAGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCg    <  2:314320/140‑1 (MQ=255)

                                                                                                                                 ggtagtGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCa  <  1:480998/140‑1 (MQ=255)

                                                                                                                                 ggtagtGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCa  <  1:699618/140‑1 (MQ=255)

                                                                                                                                 ggtagtGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCa  <  2:258287/140‑1 (MQ=255)

CCGCACCTGATCCCAAGCCGCAAACGCAAAGATCGCTGGATGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCA  >  NC_000913/3201780‑3202048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A