Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F28 I1 R1 164 66.7 2276140 99.1% 2255654 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,225,837 A→G H39R (CAC→CGC)  ebgC → DUF386 domain‑containing evolved beta‑D‑galactosidase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,225,8370AG100.0% 354.6 / NA 93H39R (CAC→CGC) ebgCDUF386 domain‑containing evolved beta‑D‑galactosidase subunit beta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (50/43);  total (50/43)

ATTTGCACAGCGAGAATAAGCAATGAGGATCATCGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCACTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAG  >  NC_000913/3225700‑3225973
                                                                                                                                         |                                                                                                                                          
aTTTGCACAGCGAGAATAAGCAATGAGGATCATCGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCt                                                                                                                                          >  1:377417/1‑140 (MQ=255)
    gCACAGCGAGAATAAGCAATGAGGATCATCGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCAt                                                                                                                                      <  2:525450/140‑1 (MQ=255)
          cGAGAATAAGCAATGAGGATCATCGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAACCTGGCGTCGCCCGCTCCATCGGTGa                                                                                                                                <  2:383518/140‑1 (MQ=255)
            agaATAAGCAATGAGGATCATCGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACt                                                                                                                              <  1:705852/140‑1 (MQ=255)
              aaTAAGCAATGAGGATCATCGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCa                                                                                                                            <  2:141752/140‑1 (MQ=255)
                  aGCAATGAGGATCATCGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGa                                                                                                                        >  2:646339/1‑140 (MQ=255)
                       tGAGGATCATCGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTAc                                                                                                                   >  1:1086688/1‑140 (MQ=255)
                       tGAGGATCATCGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTAc                                                                                                                   >  2:202744/1‑140 (MQ=255)
                        gAGGATCATCGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTAcc                                                                                                                  >  1:208976/1‑140 (MQ=255)
                         aGGATCATCGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACcg                                                                                                                 >  2:348546/1‑140 (MQ=255)
                           gATCATCGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAACTATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACcgcg                                                                                                               <  2:784326/140‑1 (MQ=255)
                           gATCATCGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACcgcg                                                                                                               >  2:760197/1‑140 (MQ=255)
                           gATCATCGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACcgcg                                                                                                               >  1:784326/1‑140 (MQ=255)
                               atcGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACtt                                                                                                                     <  1:338703/130‑1 (MQ=255)
                               atcGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACtt                                                                                                                     >  2:338703/1‑130 (MQ=255)
                               atcGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑ga                                                                                                         >  2:729345/1‑140 (MQ=255)
                                   aTAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑Gagaca                                                                                                     >  2:709610/1‑140 (MQ=255)
                                   aTAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑Gagaca                                                                                                     <  1:729345/140‑1 (MQ=255)
                                       cTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACt                                                                                                 >  2:222220/1‑140 (MQ=255)
                                                gTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCg                                                                                        <  2:395131/140‑1 (MQ=255)
                                                 ttCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGa                                                                                       >  1:698226/1‑140 (MQ=255)
                                                 ttCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGa                                                                                       >  1:910256/1‑140 (MQ=255)
                                                 ttCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGa                                                                                       >  1:411170/1‑140 (MQ=255)
                                                 ttCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGa                                                                                       >  1:314827/1‑140 (MQ=255)
                                                 ttCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGa                                                                                       >  1:1030464/1‑140 (MQ=255)
                                                  tCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGAt                                                                                      >  2:357633/1‑140 (MQ=255)
                                                  tCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGAt                                                                                      >  2:361172/1‑140 (MQ=255)
                                                  tCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGAt                                                                                      >  2:270200/1‑140 (MQ=255)
                                                  tCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGAt                                                                                      <  2:148323/140‑1 (MQ=255)
                                                   ccGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATg                                                                                     <  1:646339/140‑1 (MQ=255)
                                                   ccGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATg                                                                                     <  2:411170/140‑1 (MQ=255)
                                                   ccGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATg                                                                                     <  2:1090881/140‑1 (MQ=255)
                                                    cGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATgc                                                                                    <  2:454539/140‑1 (MQ=255)
                                                    cGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATgc                                                                                    <  2:910256/140‑1 (MQ=255)
                                                     gCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATgcg                                                                                   <  2:377417/140‑1 (MQ=255)
                                                     gCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATgcg                                                                                   <  1:202744/140‑1 (MQ=255)
                                                         gATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTAt                                                                               >  2:977471/1‑140 (MQ=255)
                                                            ttACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTa                                                                            <  1:760197/140‑1 (MQ=255)
                                                                    ctGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAGAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGGG‑‑GAGACAGACTCCCCGACCGATGCGCTATTTACCAGGCAt                                                                    >  2:1004372/1‑140 (MQ=255)
                                                                    ctGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCAt                                                                    >  1:358286/1‑140 (MQ=255)
                                                                    ctGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCAt                                                                    >  1:37802/1‑140 (MQ=255)
                                                                     tGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATc                                                                   >  2:472526/1‑140 (MQ=255)
                                                                         aagaagTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACg                                                               <  2:314827/140‑1 (MQ=255)
                                                                         aagaagTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACg                                                               <  1:709610/140‑1 (MQ=255)
                                                                         aagaagTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACg                                                               <  1:415596/140‑1 (MQ=255)
                                                                         aagaagTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACg                                                               <  2:1086688/140‑1 (MQ=255)
                                                                         aagaagTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACg                                                               <  1:856184/140‑1 (MQ=255)
                                                                              gTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTAtt                                                          <  1:348546/140‑1 (MQ=255)
                                                                                 gCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTg                                                       >  1:856123/1‑140 (MQ=255)
                                                                                 gCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTg                                                       >  1:574001/1‑140 (MQ=255)
                                                                                 gCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTg                                                       <  1:363284/140‑1 (MQ=255)
                                                                                 gCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTg                                                       >  1:97043/1‑140 (MQ=255)
                                                                                 gCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTGACCGGGCATCGACGCTATTTTg                                                       >  1:803888/1‑140 (MQ=255)
                                                                                   aaCGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGaa                                                     <  2:856123/140‑1 (MQ=255)
                                                                                   aaCGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGaa                                                     <  1:357633/140‑1 (MQ=255)
                                                                                   aaCGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGaa                                                     <  2:97043/140‑1 (MQ=255)
                                                                                    aCGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAg                                                    <  2:698226/140‑1 (MQ=255)
                                                                                    aCGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAg                                                    <  2:226070/140‑1 (MQ=255)
                                                                                      gCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTg                                                  >  2:781190/1‑140 (MQ=255)
                                                                                      gCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTg                                                  >  1:432211/1‑140 (MQ=255)
                                                                                      gCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTg                                                  <  2:803888/140‑1 (MQ=255)
                                                                                        tGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCa                                                <  1:388921/140‑1 (MQ=255)
                                                                                        tGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCa                                                >  2:879863/1‑140 (MQ=255)
                                                                                        tGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCa                                                >  2:52406/1‑140 (MQ=255)
                                                                                          cGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTGGAGAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCa                                                >  2:18044/1‑140 (MQ=255)
                                                                                             tGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATtact                                           <  1:222220/140‑1 (MQ=255)
                                                                                                   gATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGc                                     <  2:37802/140‑1 (MQ=255)
                                                                                                    aTTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCa                                    >  2:30263/1‑140 (MQ=255)
                                                                                                    aTTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCa                                    >  2:177591/1‑140 (MQ=255)
                                                                                                    aTTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCa                                    >  1:883318/1‑140 (MQ=255)
                                                                                                      tGAAAATATCGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAg                                  >  1:900825/1‑140 (MQ=255)
                                                                                                              tCGACAACATTCAGCCTGGCGTCGCTCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCaa                          >  2:851585/1‑140 (MQ=255)
                                                                                                               cGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCaaa                         >  1:368931/1‑140 (MQ=255)
                                                                                                               cGACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCaaa                         >  2:1074645/1‑140 (MQ=255)
                                                                                                                gACAACATTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCaaaa                        <  1:1004372/140‑1 (MQ=255)
                                                                                                                  caacaTTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCaaaaaa                      <  2:358286/140‑1 (MQ=255)
                                                                                                                     caTTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCaaa                         <  1:162469/134‑1 (MQ=255)
                                                                                                                     caTTCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCaaa                         >  2:162469/1‑134 (MQ=255)
                                                                                                                       ttCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGaa                 >  1:1051841/1‑140 (MQ=255)
                                                                                                                        tCAGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAACAAAAAATTGAat                >  1:241559/1‑140 (MQ=255)
                                                                                                                          aGCCTGGCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAatat              >  2:399710/1‑140 (MQ=255)
                                                                                                                               ggCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGcc         >  2:882018/1‑140 (MQ=255)
                                                                                                                                gCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATgcg           <  1:882472/137‑1 (MQ=255)
                                                                                                                                gCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATgcg           >  2:882472/1‑137 (MQ=255)
                                                                                                                                gCGTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCg        >  1:625173/1‑140 (MQ=255)
                                                                                                                                gCGTCGCCCGCTCCATCGGGGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCg        >  2:523558/1‑140 (MQ=255)
                                                                                                                                  gTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGaa      <  2:432211/140‑1 (MQ=255)
                                                                                                                                  gTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGaa      <  2:1051841/140‑1 (MQ=255)
                                                                                                                                  gTCGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGaa      >  1:768209/1‑140 (MQ=255)
                                                                                                                                    cGCCCGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAACAAAAAATTGAATATGCGCCGAAag    <  2:241559/140‑1 (MQ=255)
                                                                                                                                      cccGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAagag  <  1:879863/140‑1 (MQ=255)
                                                                                                                                      cccGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAagag  <  1:130145/140‑1 (MQ=255)
                                                                                                                                      cccGCTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAagag  <  1:882018/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                          
ATTTGCACAGCGAGAATAAGCAATGAGGATCATCGATAACTTAGAACAGTTCCGCCAGATTTACGCCTCTGGCAAGAAGTGGCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCACTCCATCGGTGACTCATTGACTTACCGCGTG‑‑GAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAG  >  NC_000913/3225700‑3225973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A