Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	164	66.7	2276140	99.1%	2255654	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,233,669	G→A	A669T (GCG→ACG)	fadH →	2,4‑dienoyl‑CoA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,233,669	0	G	A	100.0%	109.3 / NA	32	A669T (GCG→ACG)	fadH	2,4‑dienoyl‑CoA reductase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (18/14); total (18/14)

TCTGCGCAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTC  >  NC_000913/3233532‑3233803

tCTGCGCAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGacg                                                                                                                                      >  1:919096/1‑140 (MQ=255)

  tGCGCAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGACGCt                                                                                                                                    >  2:803182/1‑140 (MQ=255)

  tGCGCAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGACGCt                                                                                                                                    >  2:501739/1‑140 (MQ=255)

   gcgcAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGACGCTg                                                                                                                                   >  2:970914/1‑140 (MQ=255)

   gcgcAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGACGCTg                                                                                                                                   <  1:991227/140‑1 (MQ=255)

    cgcAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGTAGCTGGACCCACGACGGGCAATTTCCCCGGGAACACGGCTGACGCTgg                                                                                                                                  >  2:525953/1‑140 (MQ=255)

    cgcAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGACGCTgg                                                                                                                                  <  2:919096/140‑1 (MQ=255)

      cAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGACGCTGgag                                                                                                                                <  2:888215/140‑1 (MQ=255)

             agagCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGACGCTGGAGATTTaaa                                                                                                                         <  1:310389/140‑1 (MQ=255)

             agagCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGACGCTGGAGATTTaaa                                                                                                                         <  2:216264/140‑1 (MQ=255)

             agagCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGACGCTGGAGATTTaaa                                                                                                                         <  2:1022460/140‑1 (MQ=255)

              gagCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGACGCTGGAGATTTAAAt                                                                                                                        >  1:543201/1‑140 (MQ=255)

              gagCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGACGCTGGAGATTTAAAt                                                                                                                        >  1:58909/1‑140 (MQ=255)

                           cgcTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGACGCTGGAGATTTAAATCGTTATTTGAAgc                                                                                                           >  2:177533/1‑140 (MQ=255)

                                gcgcAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGACGCTGGAGATTTAAATCGTTATTTGAAGCGCCgg                                                                                                      >  2:1083874/1‑140 (MQ=255)

                                       cGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGACGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAAc                                                                                               <  2:800896/140‑1 (MQ=255)

                                                atAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGACGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGc                                                                                      >  1:669003/1‑140 (MQ=255)

                                                      ggAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGACGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTcg                                                                                >  2:478905/1‑140 (MQ=255)

                                                      ggAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGACGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTcg                                                                                <  2:58909/140‑1 (MQ=255)

                                                          aaCGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGACGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGtt                                                                            <  1:478905/140‑1 (MQ=255)

                                                           aCGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGACGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTa                                                                           <  2:669003/140‑1 (MQ=255)

                                                                 cATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGACGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAAt                                                                     >  2:894658/1‑140 (MQ=255)

                                                                             ggcTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGACGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACaa                                                               <  2:81175/134‑1 (MQ=255)

                                                                             ggcTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGACGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACaa                                                               >  1:81175/1‑134 (MQ=255)

                                                                                              ggAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGACGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATcgcg                                        >  2:1036342/1‑140 (MQ=255)

                                                                                                 gCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGACGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTg                                     >  1:147779/1‑140 (MQ=255)

                                                                                                    ggACGCACGACGGGCAATTGCCCAGGGAACACGGCTGACGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCa                                  >  1:147755/1‑140 (MQ=255)

                                                                                                          acgacgGTCAATTGCCCAGGGAACACGGCTGACGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAAttttt                            >  2:645903/1‑140 (MQ=255)

                                                                                                          acgacgGGCAATTGCCCAGGGAACACGGCTGACGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAAttttt                            >  2:781518/1‑140 (MQ=255)

                                                                                                            gacgGGCAATTGCCCAGGGAACACGGCTGACGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTa                          <  1:781518/140‑1 (MQ=255)

                                                                                                              cgGGCAATTGCCCAGGGAACACGGCTGACGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGc                        <  1:645903/140‑1 (MQ=255)

                                                                                                               gggCAATTGCCCAGGGAACACGGCTGACGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCg                       <  2:147779/140‑1 (MQ=255)

                                                                                                                               aaCACGGCTGACGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAAtt       >  2:316515/1‑140 (MQ=255)

                                                                                                                               aaCACGGCTGACGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAAtt       >  1:126729/1‑140 (MQ=255)

                                                                                                                                  acGGCTGACGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTcc    >  1:922764/1‑140 (MQ=255)

                                                                                                                                    ggCTGACGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCctc  >  2:396561/1‑140 (MQ=255)

TCTGCGCAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTC  >  NC_000913/3233532‑3233803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A