Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	164	66.7	2276140	99.1%	2255654	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,546,408	T→C	intergenic (+209/‑151)	nfuA → / → gntT	iron‑sulfur cluster carrier protein NfuA/high‑affinity gluconate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,546,408	0	T	C	100.0%	302.7 / NA	80	intergenic (+209/‑151)	nfuA/gntT	iron‑sulfur cluster carrier protein NfuA/high‑affinity gluconate transporter
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (38/42); total (38/42)

AGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGACGTTACCCATAACAAATGAA  >  NC_000913/3546272‑3546544

aGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCAcc                                                                                                                                       <  2:112411/140‑1 (MQ=255)

    gTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAAc                                                                                                                                   <  2:152400/140‑1 (MQ=255)

     ttGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACa                                                                                                                                  <  2:329591/140‑1 (MQ=255)

      tGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCAcc                                                                                                                                       >  1:138082/1‑134 (MQ=255)

      tGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCAcc                                                                                                                                       <  2:138082/134‑1 (MQ=255)

      tGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTATCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACAt                                                                                                                                 >  1:1119985/1‑140 (MQ=255)

       ggCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTTTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATa                                                                                                                                <  1:652528/140‑1 (MQ=255)

       ggCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATa                                                                                                                                <  2:390135/140‑1 (MQ=255)

         cgcAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACt                                                                                                                              <  2:72262/140‑1 (MQ=255)

           cagcaTTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTcc                                                                                                                            <  1:953088/140‑1 (MQ=255)

            agcaTTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCt                                                                                                                           <  1:1036496/140‑1 (MQ=255)

                 tGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTGCCCGTATCATTCACATGGGCACCAAACATACTCCTGACAt                                                                                                                      <  2:1007117/140‑1 (MQ=255)

                      tGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGAc                                                                                                                 >  2:943010/1‑140 (MQ=255)

                       gTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACt                                                                                                                <  2:876198/140‑1 (MQ=255)

                        tCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTa                                                                                                               >  1:339755/1‑140 (MQ=255)

                        tCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTa                                                                                                               <  1:659846/140‑1 (MQ=255)

                         cAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTAc                                                                                                              <  2:629618/140‑1 (MQ=255)

                          aaaTCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGTCACCAAACATACTCCTGACATCTGACTACa                                                                                                             <  1:216882/140‑1 (MQ=255)

                           aaTCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACaa                                                                                                            >  2:683872/1‑140 (MQ=255)

                               cTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACaataat                                                                                                        >  2:707212/1‑140 (MQ=255)

                                    ttCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGtt                                                                                                   <  1:986452/140‑1 (MQ=255)

                                    ttCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGtt                                                                                                   <  1:519855/140‑1 (MQ=255)

                                      cGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGtttt                                                                                                 <  2:174658/140‑1 (MQ=255)

                                       gTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTa                                                                                                <  1:778666/140‑1 (MQ=255)

                                        tttCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAg                                                                                               <  1:868138/140‑1 (MQ=255)

                                        tttCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAg                                                                                               >  1:933460/1‑140 (MQ=255)

                                             ccGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGt                                                                                          <  2:933460/140‑1 (MQ=255)

                                                  gtaataTGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAg                                                                                     >  2:346359/2‑140 (MQ=255)

                                                  ataataTGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAg                                                                                     >  1:315922/1‑140 (MQ=255)

                                                         gACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCgtggt                                                                              <  1:385629/140‑1 (MQ=255)

                                                           ccAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGc                                                                            >  2:599903/1‑140 (MQ=255)

                                                           ccAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGc                                                                            <  1:346359/140‑1 (MQ=255)

                                                           ccAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTGAGTGGGTATCAGTCGTGGTGc                                                                            >  2:1033956/1‑140 (MQ=255)

                                                             aaCCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCgtggt                                                                              >  2:1023843/1‑136 (MQ=255)

                                                             aaCCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCgtggt                                                                              <  1:1023843/136‑1 (MQ=255)

                                                             aaCCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCg                                                                          >  1:330787/1‑140 (MQ=255)

                                                              aCCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGc                                                                         >  1:260389/1‑140 (MQ=255)

                                                               ccTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCa                                                                        >  2:1112115/1‑140 (MQ=255)

                                                               ccTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCa                                                                        >  1:933417/1‑140 (MQ=255)

                                                               ccTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCa                                                                        >  1:404442/1‑140 (MQ=255)

                                                                 tctcATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAat                                                                      >  1:1095304/1‑140 (MQ=255)

                                                                   tcATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAatat                                                                    >  2:5565/1‑140 (MQ=255)

                                                                     aTAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATAtct                                                                  <  1:125564/140‑1 (MQ=255)

                                                                          ttaaatttaCCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGtt                                                             >  1:981277/1‑140 (MQ=255)

                                                                           taaatttaCCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATTCTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTc                                                            <  2:1080816/140‑1 (MQ=255)

                                                                             aatttaCCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAatat                                                                    <  2:660570/130‑1 (MQ=255)

                                                                             aatttaCCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAatat                                                                    >  1:660570/1‑130 (MQ=255)

                                                                               tttaCCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGa                                                        <  2:330787/140‑1 (MQ=255)

                                                                               tttaCCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGa                                                        <  2:723855/140‑1 (MQ=255)

                                                                                ttaCCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGAt                                                       <  2:404442/140‑1 (MQ=255)

                                                                                   ccccGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTgg                                                    <  1:388214/140‑1 (MQ=255)

                                                                                   ccccGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTgg                                                    <  2:1119985/140‑1 (MQ=255)

                                                                                   ccccGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTgg                                                    >  2:388214/1‑140 (MQ=255)

                                                                                     ccGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGa                                                  <  2:1095304/140‑1 (MQ=255)

                                                                                        ctctGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATaa                                               >  1:624796/1‑140 (MQ=255)

                                                                                             gTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTaga                                          >  1:873369/1‑140 (MQ=255)

                                                                                                  tctcAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTgg                                                    >  1:592874/1‑125 (MQ=255)

                                                                                                  tctcAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTgg                                                    <  2:592874/125‑1 (MQ=255)

                                                                                                   ctcAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTgt                                    >  1:1120934/1‑140 (MQ=255)

                                                                                                     cAAACGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTgtcg                                  >  1:245514/1‑140 (MQ=255)

                                                                                                       aaCGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTgtcgtc                                >  1:1110682/1‑140 (MQ=255)

                                                                                                       aaCGCCAGATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTgtcgtc                                >  1:849740/1‑140 (MQ=255)

                                                                                                              gacgTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAAt                         <  2:981277/137‑1 (MQ=255)

                                                                                                              gATGTTACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAAt                         <  2:849740/140‑1 (MQ=255)

                                                                                                                   tACCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGACg                    <  2:339755/140‑1 (MQ=255)

                                                                                                                    aCCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGACGt                   >  2:85749/1‑140 (MQ=255)

                                                                                                                    aCCCGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGACGt                   >  2:1011782/1‑140 (MQ=255)

                                                                                                                     cccGTATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGACGtt                  <  2:58811/140‑1 (MQ=255)

                                                                                                                         tATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGACGTTAccc              >  1:979735/1‑140 (MQ=255)

                                                                                                                         tATCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGACGTTAccc              >  1:650057/1‑140 (MQ=255)

                                                                                                                          aTCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGaaaa                          <  2:941931/127‑1 (MQ=255)

                                                                                                                          aTCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGaaaa                          >  1:941931/1‑127 (MQ=255)

                                                                                                                           tCATTCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGACGTTACCCAt            <  2:933417/140‑1 (MQ=255)

                                                                                                                              ttCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGACGtt                  <  2:783566/131‑1 (MQ=255)

                                                                                                                              ttCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGACGtt                  >  1:783566/1‑131 (MQ=255)

                                                                                                                              ttCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGACGTTACCCATAAc         >  1:545357/1‑140 (MQ=255)

                                                                                                                              ttCACATGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGACGTCAGAAAATTGACGTTACCCATAAc         <  2:624796/140‑1 (MQ=255)

                                                                                                                                   aTGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGACGTTACCCATAACAAATg    >  1:72299/1‑140 (MQ=255)

                                                                                                                                    tGGGCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGACGTTACCCATAACAAATGa   <  2:537639/140‑1 (MQ=255)

                                                                                                                                     gggCACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGACGTTACCCATAACAAATGaa  >  1:611154/1‑140 (MQ=255)

AGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGACGTTACCCATAACAAATGAA  >  NC_000913/3546272‑3546544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A