Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	164	66.7	2276140	99.1%	2255654	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,574,808	T→C	E24G (GAG→GGG)	asd ←	aspartate‑semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,574,808	0	T	C	100.0%	251.8 / NA	68	E24G (GAG→GGG)	asd	aspartate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (34/34); total (34/34)

TATCGAGGGCCTTTAGCGCCTCCAGATCAAAGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCTCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTT  >  NC_000913/3574677‑3574943

tatCGAGGGCCTTTAGCGCCTCCAGATCAAAGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAAcc                                                                                                                                 >  2:301296/1‑140 (MQ=255)

   cGAGGGCCTTTAGCGCCTCCAGATCAAAGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATg                                                                                                                              <  1:301296/140‑1 (MQ=255)

   cGAGGGCCTTTAGCGCCTCCAGATCAAAGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATg                                                                                                                              <  1:224579/140‑1 (MQ=255)

       ggCCTTTAGCGCCTCCAGATCAAAGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGtt                                                                                                                          >  2:847885/1‑140 (MQ=255)

         ccTTTAGCGCCTCCAGATCAAAGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGc                                                                                                                        >  1:769226/1‑140 (MQ=255)

           tttAGCGCCTCCAGATCAAAGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCAt                                                                                                                      <  1:682669/140‑1 (MQ=255)

           tttAGCGCCTCCAGATCAAAGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCAt                                                                                                                      <  1:847885/140‑1 (MQ=255)

                cgcCTCCAGATCAAAGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGaa                                                                                                                 <  2:769226/140‑1 (MQ=255)

                  ccTCCAGATCAAAGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACg                                                                                                               >  1:305282/1‑140 (MQ=255)

                   cTCCAGATCAAAGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGc                                                                                                                             >  2:899119/1‑125 (MQ=255)

                   cTCCAGATCAAAGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGc                                                                                                                             <  1:899119/125‑1 (MQ=255)

                   cTCCAGATCAAAGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACgg                                                                                                              >  2:52780/1‑140 (MQ=255)

                    tCCAGATCAAAGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGa                                                                                                             <  2:9239/140‑1 (MQ=255)

                    tCCAGATCAAAGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGa                                                                                                             >  1:231204/1‑140 (MQ=255)

                    tCCAGATCAAAGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGa                                                                                                             >  1:277531/1‑140 (MQ=255)

                             aaGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGACTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCAt                                                                                                    >  2:556488/1‑140 (MQ=255)

                                   tCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGa                                                                                                        >  2:941226/1‑130 (MQ=255)

                                   tCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGa                                                                                                        <  1:941226/130‑1 (MQ=255)

                                   tCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACcgcg                                                                                              >  2:80959/1‑140 (MQ=255)

                                    ccTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGc                                                                                                           >  2:510880/1‑126 (MQ=255)

                                    ccTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGc                                                                                                           <  1:510880/126‑1 (MQ=255)

                                             tgCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATa                                                                                    >  2:747359/1‑140 (MQ=255)

                                              gCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACTATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATaa                                                                                   >  2:1128335/1‑140 (MQ=255)

                                                  gTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAAcc                                                                               >  1:1040311/1‑140 (MQ=255)

                                                      ttCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACa                                                                           <  1:406901/140‑1 (MQ=255)

                                                       tCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACAt                                                                          >  1:1050853/1‑140 (MQ=255)

                                                            cAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTc                                                                     >  2:893397/1‑140 (MQ=255)

                                                                      cgcAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGttt                                                           >  1:93745/1‑140 (MQ=255)

                                                                      cgcAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGttt                                                           >  1:537909/1‑140 (MQ=255)

                                                                         aGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGtttttt                                                        <  1:1029607/140‑1 (MQ=255)

                                                                         aGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGtttttt                                                        <  1:1113315/140‑1 (MQ=255)

                                                                         aGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGtttttt                                                        <  2:537909/140‑1 (MQ=255)

                                                                                ccAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCa                                                 >  2:1129349/1‑140 (MQ=255)

                                                                                    gCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGa                                             >  1:499145/1‑140 (MQ=255)

                                                                                    gCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGa                                             >  1:1002517/1‑140 (MQ=255)

                                                                                     cTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGAt                                            >  1:968517/1‑140 (MQ=255)

                                                                                     cTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGAt                                            <  2:372677/140‑1 (MQ=255)

                                                                                     cTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGAt                                            >  2:10437/1‑140 (MQ=255)

                                                                                        agaAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGAtgtg                                         <  1:1129349/140‑1 (MQ=255)

                                                                                        agaAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGAtgtg                                         <  1:893397/140‑1 (MQ=255)

                                                                                          aaGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACTATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGAtgtgtg                                       <  1:1128335/140‑1 (MQ=255)

                                                                                          aaGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGAtgtgtg                                       <  1:728775/140‑1 (MQ=255)

                                                                                          aaGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGAtgtgtg                                       <  1:495565/140‑1 (MQ=255)

                                                                                          aaGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGAtgtgtg                                       <  2:93745/140‑1 (MQ=255)

                                                                                          aaGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGAtgtgtg                                       <  2:305282/140‑1 (MQ=255)

                                                                                          aaGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGAtgtgtg                                       <  1:52780/140‑1 (MQ=255)

                                                                                          aaGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGAtgtgtg                                       <  1:80959/140‑1 (MQ=255)

                                                                                          aaGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGAtgtgtg                                       <  2:1040311/140‑1 (MQ=255)

                                                                                                  aagaagACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGAtgtgtg                                       >  1:759490/1‑132 (MQ=255)

                                                                                                  aagaagACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGAtgtgtg                                       <  2:415764/132‑1 (MQ=255)

                                                                                                  aagaagACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGAtgtgtg                                       <  2:759490/132‑1 (MQ=255)

                                                                                                  aagaagACAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGAtgtgtg                                       >  1:415764/1‑132 (MQ=255)

                                                                                                    gaagaCAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAAt                             >  2:470190/1‑140 (MQ=255)

                                                                                                       gaCAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGGGCCGACCATCCCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAAtgtg                          <  2:277531/140‑1 (MQ=255)

                                                                                                       gaCAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAAtgtg                          >  2:667673/1‑140 (MQ=255)

                                                                                                       gaCAGGGCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAAtgtg                          >  1:121349/1‑140 (MQ=255)

                                                                                                           gggCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCgg                      >  1:646686/1‑140 (MQ=255)

                                                                                                            ggCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGt                     >  1:470041/1‑140 (MQ=255)

                                                                                                            ggCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGt                     <  2:646686/140‑1 (MQ=255)

                                                                                                            ggCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGt                     <  2:121349/140‑1 (MQ=255)

                                                                                                            ggCGAATGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGt                     >  1:67562/1‑140 (MQ=255)

                                                                                                                 aTGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTctcct                <  1:10437/140‑1 (MQ=255)

                                                                                                                  tGGCGTCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTctcctc               <  1:156707/140‑1 (MQ=255)

                                                                                                                       tCGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGc          <  2:67562/140‑1 (MQ=255)

                                                                                                                        cGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGca         <  2:1050853/140‑1 (MQ=255)

                                                                                                                        cGAAGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGca         <  1:667673/140‑1 (MQ=255)

                                                                                                                          aaGTCGCGCCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGcaca       <  2:394441/140‑1 (MQ=255)

                                                                                                                               gcgcCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCttt  >  2:748816/1‑140 (MQ=255)

                                                                                                                               gcgcCCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCttt  >  2:394176/1‑140 (MQ=255)

TATCGAGGGCCTTTAGCGCCTCCAGATCAAAGGCATCCTGAAGTGTGCCAGTGGTTCCGCCAAAAGACGGCGCAGCCTGGCCAAGCTGAGAAGTAGAAAAGAAGACAGGGCGAATGGCGTCGAAGTCGCGCTCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTT  >  NC_000913/3574677‑3574943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A