Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	126	51.4	1736588	99.0%	1719222	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,921,048	G→A	D276N (GAT→AAT)	rsmF →	16S rRNA m(5)C1407 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,921,048	0	G	A	100.0%	98.8 / NA	29	D276N (GAT→AAT)	rsmF	16S rRNA m(5)C1407 methyltransferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/12); total (17/12)

TTACGTCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGTAAAACTCAGGCGATTCCCGCCTTACCCGCCCCCA  >  NC_000913/1920928‑1921183

ttACGTCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAAtc                                                                                                                                      <  1:105736/124‑1 (MQ=255)

ttACGTCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAAtc                                                                                                                                      >  2:105736/1‑124 (MQ=255)

    gTCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGcaaacaaa                                                                                                                  >  2:811859/1‑140 (MQ=255)

      ccTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAgc                                                                                                                >  1:277779/1‑140 (MQ=255)

      ccTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAgc                                                                                                                >  2:730416/1‑140 (MQ=255)

       cTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAgcg                                                                                                               <  2:277779/140‑1 (MQ=255)

       cTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAgcg                                                                                                               <  1:862419/140‑1 (MQ=255)

          gtggtACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTg                                                                                                            <  2:660106/140‑1 (MQ=255)

                cGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCgaa                                                                                                      >  1:740426/1‑140 (MQ=255)

                  cTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCgaaga                                                                                                    >  2:64955/1‑140 (MQ=255)

                                    aCCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGtttt                                                                                  >  1:27404/1‑140 (MQ=255)

                                                gaagaaAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCcac                                                                             >  1:529393/1‑133 (MQ=255)

                                                gaagaaAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCcac                                                                             <  2:529393/133‑1 (MQ=255)

                                                gaagaaAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTa                                                                      >  1:318830/1‑140 (MQ=255)

                                                              cGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGct                                                        <  1:64955/140‑1 (MQ=255)

                                                                tttGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGa                                                                   <  1:635193/127‑1 (MQ=255)

                                                                tttGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGa                                                                   >  2:635193/1‑127 (MQ=255)

                                                                tttGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGcttc                                                      >  2:596941/1‑140 (MQ=255)

                                                                  tGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGcttctt                                                    <  2:740426/140‑1 (MQ=255)

                                                                                  agaCTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGt                                    <  2:134835/140‑1 (MQ=255)

                                                                                    aCTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGTaa                                  >  1:96998/1‑140 (MQ=255)

                                                                                    aCTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGTaa                                  >  2:21653/1‑140 (MQ=255)

                                                                                            cGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGTAAAACTCAgg                          <  1:47708/140‑1 (MQ=255)

                                                                                              aCGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGTAAAAcagacgag                        <  2:27404/140‑8 (MQ=255)

                                                                                              aCGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGTAAAACTCAGGCg                        >  2:711945/1‑140 (MQ=255)

                                                                                                 cAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGTAAAACTCAGGCGAtt                     <  1:596941/140‑1 (MQ=255)

                                                                                                            ttACCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGTAAAACTCAGGCGATTCCCGCCTTAcc          >  2:440135/1‑140 (MQ=255)

                                                                                                              aCCACTTGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGTAAAACTCAGGCGATTCCCGCCTTACCCg        >  2:559452/1‑140 (MQ=255)

                                                                                                                    tGGCAATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGTAAAACTCAGGCGATTCCCGCCTTACCCGCCCCCa  >  1:528598/1‑140 (MQ=255)

TTACGTCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGTAAAACTCAGGCGATTCCCGCCTTACCCGCCCCCA  >  NC_000913/1920928‑1921183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A