Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	126	51.4	1736588	99.0%	1719222	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,276,206	A→G	T312A (ACC→GCC)	yejE →	putative oligopeptide ABC transporter membrane subunit YejE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,276,206	0	A	G	100.0%	167.1 / NA	48	T312A (ACC→GCC)	yejE	putative oligopeptide ABC transporter membrane subunit YejE
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (21/27); total (21/27)

CGTTTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTT  >  NC_000913/2276069‑2276331

cGTTTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGcc                                                                                                                             >  2:367890/1‑140 (MQ=255)

                ttCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTgg                                                                                                             >  2:516563/1‑140 (MQ=255)

                ttCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTgg                                                                                                             >  1:77185/1‑140 (MQ=255)

                  cGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCg                                                                                                           <  2:830542/140‑1 (MQ=255)

                  cGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCg                                                                                                           <  2:687531/140‑1 (MQ=255)

                         caccCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGAtattat                                                                                                    <  1:516563/140‑1 (MQ=255)

                          accCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCg                                                                                                           <  1:867683/132‑1 (MQ=255)

                          accCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCg                                                                                                           >  2:867683/1‑132 (MQ=255)

                            ccTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGt                                                                                                 >  1:440960/1‑140 (MQ=255)

                            ccTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGt                                                                                                 <  1:649923/140‑1 (MQ=255)

                               gACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCtt                                                                                              >  1:570168/1‑140 (MQ=255)

                                aCCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTgg                                                                                                             >  2:594432/1‑124 (MQ=255)

                                aCCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTgg                                                                                                             <  1:594432/124‑1 (MQ=255)

                                 ccTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTg                                                                                            <  2:440960/140‑1 (MQ=255)

                                 ccTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTg                                                                                            <  2:570168/140‑1 (MQ=255)

                                   tcgctcgATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCt                                                                                          >  1:115058/1‑140 (MQ=255)

                                     gctcgATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGa                                                                                        >  1:698027/1‑140 (MQ=255)

                                      ctcgATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGAtatt                                                                                                      <  2:760179/125‑1 (MQ=255)

                                      ctcgATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGAtatt                                                                                                      >  1:760179/1‑125 (MQ=255)

                                      ctcgATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTg                                                                                         <  1:312677/138‑1 (MQ=255)

                                      ctcgATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTg                                                                                         >  2:312677/1‑138 (MQ=255)

                                          aTTTCCTCGGCTTCGGTCTGCCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCttt                                                                                   <  1:202867/140‑1 (MQ=255)

                                          aTTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTg                                                                                            <  2:429025/131‑1 (MQ=255)

                                          aTTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTg                                                                                            <  2:394042/131‑1 (MQ=255)

                                          aTTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTg                                                                                            >  1:429025/1‑131 (MQ=255)

                                          aTTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTg                                                                                            >  1:394042/1‑131 (MQ=255)

                                          aTTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCttt                                                                                   <  1:856570/140‑1 (MQ=255)

                                           tttCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTa                                                                                  <  2:146124/140‑1 (MQ=255)

                                            ttCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTAt                                                                                 <  1:78484/140‑1 (MQ=255)

                                               cTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTgg                                                                              >  1:51752/1‑140 (MQ=255)

                                                       cGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCg                                                                      >  1:146986/1‑140 (MQ=255)

                                                                        cACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGAt                                                     <  1:367890/140‑1 (MQ=255)

                                                                                cTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCtaataa                                             <  2:813254/140‑1 (MQ=255)

                                                                                  cGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAgg                                           <  1:253731/140‑1 (MQ=255)

                                                                                  cGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAgg                                           <  2:277726/140‑1 (MQ=255)

                                                                                  cGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAgg                                           <  2:77185/140‑1 (MQ=255)

                                                                                           gctgTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAgg                                           >  1:796264/1‑131 (MQ=255)

                                                                                           gctgTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAgg                                           <  2:796264/131‑1 (MQ=255)

                                                                                             tgTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCAt                                >  2:539090/1‑140 (MQ=255)

                                                                                             tgTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCAt                                <  1:539090/140‑1 (MQ=255)

                                                                                               ttACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGa                              <  2:51752/140‑1 (MQ=255)

                                                                                                   aaGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCa                          <  2:424967/140‑1 (MQ=255)

                                                                                                    aGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCaa                         >  2:122193/1‑140 (MQ=255)

                                                                                                    aGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCaa                         >  1:682408/1‑140 (MQ=255)

                                                                                                      ggAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCCCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAAc                       >  2:804149/1‑140 (MQ=255)

                                                                                                       gAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAAct                      <  1:333892/140‑1 (MQ=255)

                                                                                                                     aGGCCCCGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGa        <  2:698027/140‑1 (MQ=255)

                                                                                                                           cGTGGCTTGGGATCGCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAAttt  >  1:164185/1‑140 (MQ=255)

CGTTTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTT  >  NC_000913/2276069‑2276331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A