Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	126	51.4	1736588	99.0%	1719222	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,373,305	T→C	E78E (GAA→GAG)	pmrD ←	signal transduction protein PmrD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,373,305	0	T	C	100.0%	178.7 / NA	51	E78E (GAA→GAG)	pmrD	signal transduction protein PmrD
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/27); total (24/27)

CTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACGGCGAAGTC  >  NC_000913/2373171‑2373430

cTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCa                                                                                                                          >  2:526450/1‑140 (MQ=255)

cTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCa                                                                                                                          >  1:415796/1‑140 (MQ=255)

cTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCa                                                                                                                          >  2:719297/1‑140 (MQ=255)

cTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCa                                                                                                                          >  1:516887/1‑140 (MQ=255)

cTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTCTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCa                                                                                                                          <  2:801434/140‑1 (MQ=255)

cTTCCCGGCGGGGAAGGGATTTTTTCGCTAAAAGCACTACTGGGAGTAGCCTGTATTATGAGGGGTTGGATGCTGTTTTTCCTGCCCACGAAAAAACACCGTTACTGATTTTTCCCTGCCACTTTACACTGCCGCTCCCa                                                                                                                          <  2:651838/140‑1 (MQ=255)

      ggCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATc                                                                                                                    <  1:8888/140‑1 (MQ=255)

        cTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCgg                                                                                                                  >  2:298958/1‑140 (MQ=255)

        cTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTCCCTGCCCCCGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTTATCgg                                                                                                                  <  1:552787/140‑1 (MQ=255)

                      tttCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCtt                                                                                                    <  1:77208/140‑1 (MQ=255)

                      tttCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCtt                                                                                                    >  1:653620/1‑140 (MQ=255)

                         cGCTGAAAGCACTCCTGGGAGAAGCCTGTATTATGAGCGGTTTGTTGCTGTTTTTCCTGCCCACAACAAAACAACGTTCCGGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGcc                                                                                                 <  2:393315/140‑1 (MQ=255)

                         cGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGcc                                                                                                 <  2:655531/140‑1 (MQ=255)

                            tGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCgag                                                                                              <  1:298958/140‑1 (MQ=255)

                              aaaGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGagag                                                                                            <  1:526450/140‑1 (MQ=255)

                                aGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGa                                                                                          <  1:719297/140‑1 (MQ=255)

                                          ggAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGt                                                                                >  1:609027/1‑140 (MQ=255)

                                              tAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTc                                                                                     >  1:439968/1‑131 (MQ=255)

                                              tAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTc                                                                                     <  2:439968/131‑1 (MQ=255)

                                                 ccTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGc                                                                         >  2:243967/1‑140 (MQ=255)

                                                    gTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCttt                                                                      >  1:447696/1‑140 (MQ=255)

                                                     tattatGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCtttt                                                                     <  2:609027/140‑1 (MQ=255)

                                                     tattatGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCtttt                                                                     >  1:685258/1‑140 (MQ=255)

                                                         atGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACg                                                                 <  1:243967/140‑1 (MQ=255)

                                                          tGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGa                                                                >  1:630352/1‑140 (MQ=255)

                                                           gAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGAt                                                               <  2:516887/140‑1 (MQ=255)

                                                           gAGCGGGTTGATGCTGATTTTCCTGCCCACGAAAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGAt                                                               <  2:415796/140‑1 (MQ=255)

                                                               gggTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTc                                                                    >  1:522942/1‑131 (MQ=255)

                                                               gggTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTc                                                                    <  2:522942/131‑1 (MQ=255)

                                                               gggTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAAt                                                           >  2:679898/1‑140 (MQ=255)

                                                               gggTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAAt                                                           >  2:677098/1‑140 (MQ=255)

                                                               gggTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAAt                                                           >  1:660524/1‑140 (MQ=255)

                                                                 gTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATAc                                                         >  2:189158/1‑140 (MQ=255)

                                                                 gTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATAc                                                         >  1:583608/1‑140 (MQ=255)

                                                                          gATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAgcg                                                <  2:630352/140‑1 (MQ=255)

                                                                           aTTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAgcgc                                               >  2:751038/1‑140 (MQ=255)

                                                                           aTTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAgcgc                                               >  1:364865/1‑140 (MQ=255)

                                                                            ttttCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCa                                              <  1:64072/140‑1 (MQ=255)

                                                                            ttttCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCa                                              <  2:653620/140‑1 (MQ=255)

                                                                            ttttCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCa                                              <  1:189158/140‑1 (MQ=255)

                                                                                  tGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGc                                        <  1:679898/140‑1 (MQ=255)

                                                                                   gCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCa                                       <  1:196853/140‑1 (MQ=255)

                                                                                   gCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCa                                       <  2:660524/140‑1 (MQ=255)

                                                                                        cGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGc                                  <  2:443134/140‑1 (MQ=255)

                                                                                                   cGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATc                       >  1:406963/1‑140 (MQ=255)

                                                                                                      tACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATcccc                    <  2:685258/140‑1 (MQ=255)

                                                                                                        cTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCa                  >  2:680087/1‑140 (MQ=255)

                                                                                                        cTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCa                  >  2:820440/1‑140 (MQ=255)

                                                                                                             ttttCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTc             >  2:450498/1‑140 (MQ=255)

                                                                                                                       cACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACGGCGAAGt   <  2:406963/140‑1 (MQ=255)

                                                                                                                        aCTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACGGCGAAGTc  <  2:563398/140‑1 (MQ=255)

CTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACGGCGAAGTC  >  NC_000913/2373171‑2373430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A