Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F28 I1 R1 126 51.4 1736588 99.0% 1719222 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,157,798 A→G E50G (GAG→GGG)  yqhG → DUF3828 domain‑containing protein YqhG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,157,7980AG100.0% 164.0 / NA 48E50G (GAG→GGG) yqhGDUF3828 domain‑containing protein YqhG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/22);  total (26/22)

CTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCGGTTTGTGATTGTCAGGATTTTGGCGATCTGGTGCTAGA  >  NC_000913/3157665‑3157930
                                                                                                                                     |                                                                                                                                    
cTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAgggcgggc                                                                                                                                >  1:242303/1‑140 (MQ=255)
cTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAgggcgggc                                                                                                                                >  1:562220/1‑140 (MQ=255)
        agcagcCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACtt                                                                                                                        >  1:506596/1‑140 (MQ=255)
          cagcCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCt                                                                                                                      >  1:63491/1‑140 (MQ=255)
             cccTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCgg                                                                                                                                  >  2:583052/1‑125 (MQ=255)
             cccTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCgg                                                                                                                                  <  1:583052/125‑1 (MQ=255)
             cccTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTgcg                                                                                                                   >  1:771490/1‑140 (MQ=255)
             cccTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGCCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTCTTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTgcg                                                                                                                   >  2:447006/1‑140 (MQ=255)
                 ggCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACGATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAGCCGGAGGGCGGGCGATAACTTCTGCGCGTa                                                                                                               <  2:316798/140‑1 (MQ=255)
                       ttttACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAAc                                                                                                         >  1:81365/1‑140 (MQ=255)
                             cGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGgcgc                                                                                                   <  1:253121/140‑1 (MQ=255)
                                 cacGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTAc                                                                                               >  2:833728/1‑140 (MQ=255)
                                     cacaGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTg                                                                                           <  2:731018/140‑1 (MQ=255)
                                         gCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACg                                                                                       >  2:38397/1‑140 (MQ=255)
                                         gCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACg                                                                                       >  1:743122/1‑140 (MQ=255)
                                          ccccccTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGa                                                                                      >  2:40063/1‑140 (MQ=255)
                                          ccccccTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGa                                                                                      >  2:823224/1‑140 (MQ=255)
                                          ccccccTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGa                                                                                      <  1:833728/140‑1 (MQ=255)
                                          ccccccTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGa                                                                                      <  2:506596/140‑1 (MQ=255)
                                              ccTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAAt                                                                                  >  1:613986/1‑140 (MQ=255)
                                                       ccGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTg                                                                         <  1:64711/140‑1 (MQ=255)
                                                            gAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCggg                                                                    <  2:675085/140‑1 (MQ=255)
                                                            gAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCggg                                                                    <  2:434333/140‑1 (MQ=255)
                                                            gAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCggg                                                                    <  1:823224/140‑1 (MQ=255)
                                                                      tCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATtggc                                                          >  1:393345/1‑140 (MQ=255)
                                                                      tCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATtggc                                                          <  2:242303/140‑1 (MQ=255)
                                                                         ggCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATtggctgg                                                       <  1:40063/140‑1 (MQ=255)
                                                                         ggCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATtggctgg                                                       <  2:81365/140‑1 (MQ=255)
                                                                         ggCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATtggctgg                                                       <  1:415450/140‑1 (MQ=255)
                                                                             tatTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGctggctgg                                                   >  2:318285/1‑140 (MQ=255)
                                                                              atTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGa                                                  <  1:111556/140‑1 (MQ=255)
                                                                                ttATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGAtt                                                >  2:68010/1‑140 (MQ=255)
                                                                                     aGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGAt                                           >  2:647284/1‑140 (MQ=255)
                                                                                      gAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATc                                          <  2:238038/140‑1 (MQ=255)
                                                                                        aCTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCg                                        >  1:370854/1‑140 (MQ=255)
                                                                                         cTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGa                                                  <  2:96608/129‑1 (MQ=255)
                                                                                         cTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGa                                                  >  1:96608/1‑129 (MQ=255)
                                                                                         cTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCgg                                       >  2:304565/1‑140 (MQ=255)
                                                                                            taAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCg                                        <  1:764934/136‑1 (MQ=255)
                                                                                            taAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCg                                        >  2:764934/1‑136 (MQ=255)
                                                                                                    aTGCCACTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCGGTTTGTGATTGt                            <  2:171535/140‑1 (MQ=255)
                                                                                                          cTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCGGTTTGTGATTGTCAGGAt                      >  2:803649/1‑140 (MQ=255)
                                                                                                          cTGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCGGTTTGTGATTGTCAGGAt                      >  2:861753/1‑140 (MQ=255)
                                                                                                           tGCCCCTTATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCGGTTTGTGATTGTCAGGAtt                     >  1:325979/1‑140 (MQ=255)
                                                                                                                  tATTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCGGTTTGTGATTGTCAGGATTTTGGCGa              >  2:753928/1‑140 (MQ=255)
                                                                                                                   aTTTTGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCGGTTTGTGATTGTCAGGATTTTGGCGAt             <  2:325979/140‑1 (MQ=255)
                                                                                                                    ttttGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATGCAGCAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCGGTTTGTGATTGTCAGGATTTTGGCGATc            <  1:447006/140‑1 (MQ=255)
                                                                                                                       tGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCGGTTTGTGATTGTCAGGATTTTGGCGATCTgg         >  2:390868/1‑140 (MQ=255)
                                                                                                                       tGGTGAAACCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTACCGGGCAATATTGGCTGGCTGGATTATGATCCGGTTTGTGATTGTCAGGATTTTGGCGATCTgg         >  2:374657/1‑140 (MQ=255)
                                                                                                                              aCCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCGGTTTGTGATTGTCAGGATTTTGGCGATCTGGTGCTAGa  <  1:753928/140‑1 (MQ=255)
                                                                                                                              aCCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCGGTTTGTGATTGTCAGGATTTTGGCGATCTGGTGCTAGa  >  1:313819/1‑140 (MQ=255)
                                                                                                                              aCCGGAGGGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCGGTTTGTGATTGTCAGGATTTTGGCGATCTGGTGCTAGa  <  1:304565/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                    
CTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCGGTTTGTGATTGTCAGGATTTTGGCGATCTGGTGCTAGA  >  NC_000913/3157665‑3157930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A