Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	126	51.4	1736588	99.0%	1719222	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,177,350	G→A	N187N (AAC→AAT)	nudF ←	ADP‑sugar pyrophosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,177,350	0	G	A	100.0%	129.2 / NA	37	N187N (AAC→AAT)	nudF	ADP‑sugar pyrophosphatase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (13/24); total (13/24)

CGCAATTGTGAAAAGTTCATCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGTTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCC  >  NC_000913/3177213‑3177476

cgcAATTGTGAAAAGTTCATCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCAtt                                                                                                                              >  2:65849/1‑140 (MQ=255)

  cAATTGTGAAAAGTTCATCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGt                                                                                                                            <  2:558168/140‑1 (MQ=255)

            aaGTTCATCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTccc                                                                                                                  <  1:492749/140‑1 (MQ=255)

               ttCATCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCctc                                                                                                               <  1:53337/140‑1 (MQ=255)

                 cATCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCtctt                                                                                                             <  2:532105/140‑1 (MQ=255)

                 cATCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCtctt                                                                                                             <  2:50906/140‑1 (MQ=255)

                 cATCTCGCACAGGAGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCtctt                                                                                                             <  1:326268/140‑1 (MQ=255)

                  aTCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCtcttc                                                                                                            >  1:660409/1‑140 (MQ=255)

                   tctcGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCtcttct                                                                                                           <  1:65849/140‑1 (MQ=255)

                   tctcGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCtcttct                                                                                                           <  2:660409/140‑1 (MQ=255)

                      cGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTAcc                                                                                                        <  1:801737/140‑1 (MQ=255)

                       gCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTAccc                                                                                                       <  1:576696/140‑1 (MQ=255)

                         acaGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCAc                                                                                                     <  2:113103/140‑1 (MQ=255)

                             gcgcATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGt                                                                                                 >  2:34278/1‑140 (MQ=255)

                                 atcatTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGc                                                                                             <  1:18387/140‑1 (MQ=255)

                                             aaGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTc                                                                                       >  2:661918/1‑134 (MQ=255)

                                             aaGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTc                                                                                       <  1:661918/134‑1 (MQ=255)

                                             aaGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCt                                                                                 <  1:5719/140‑1 (MQ=255)

                                               gTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGTCCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTa                                                                               <  1:70628/140‑1 (MQ=255)

                                                              cTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGaa                                                                >  1:182850/1‑140 (MQ=255)

                                                                 cATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAatat                                                             >  1:469345/1‑140 (MQ=255)

                                                                       tGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGt                                                       >  1:446847/1‑140 (MQ=255)

                                                                         cccACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGttt                                                     >  2:140851/1‑140 (MQ=255)

                                                                            aCTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTtca                                                  >  1:498504/1‑140 (MQ=255)

                                                                             cTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAatat                                                             <  1:499674/128‑1 (MQ=255)

                                                                             cTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAatat                                                             >  2:499674/1‑128 (MQ=255)

                                                                             cTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTtcat                                                 >  2:481554/1‑140 (MQ=255)

                                                                                     ttAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGa                                         <  2:182850/140‑1 (MQ=255)

                                                                                      tAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGAc                                        <  2:469345/140‑1 (MQ=255)

                                                                                       aaCGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGAcc                                       <  1:481554/140‑1 (MQ=255)

                                                                                            ttGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGaa                                  <  2:446847/140‑1 (MQ=255)

                                                                                                     cagctgcagcCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTg                         <  1:34278/140‑1 (MQ=255)

                                                                                                        ctgcagcCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGAc                                        >  1:555553/1‑122 (MQ=255)

                                                                                                        ctgcagcCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGAc                                        <  2:555553/122‑1 (MQ=255)

                                                                                                             gcCATTGCAAAGCGATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTg                 >  2:727198/1‑140 (MQ=255)

                                                                                                                          gATGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCATGGCGTCCACTTCGc    <  1:33619/140‑1 (MQ=255)

                                                                                                                            tGACCGACGCTGCATTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGccc  <  2:498504/140‑1 (MQ=255)

CGCAATTGTGAAAAGTTCATCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGTTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCC  >  NC_000913/3177213‑3177476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A