Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F28 I1 R1 126 51.4 1736588 99.0% 1719222 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,192,444 (T)7→8 coding (237/630 nt) yqiJ → putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,192,4371.T98.8% 280.8 / ‑3.8 81coding (230/630 nt)yqiJputative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base T (44/36);  total (44/37)
Fisher's exact test for biased strand distribution p-value = 4.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.30e-01

ACATGCTCTCCGGTGCTCTTGATGCACATCTTGACCATTATGATTCTATAACCACAGGTCATATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGG‑‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAA  >  NC_000913/3192305‑3192567
                                                                                                                                     |                                                                                                                                   
acaTGCTCTCCGGTGCTCTTGATGCACATCTTGACCATTATGATTCTATAACCACAGGTCATATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGG‑‑ttttttt                                                                                                                             >  1:117602/1‑140 (MQ=255)
acaTGCTCTCCGGTGCTCTTGATGCACATCTTGACCATTATGATTCTATAACCACAGGTCATATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGG‑‑ttttttt                                                                                                                             <  2:117602/140‑1 (MQ=255)
 caTGCTCTCCGGTGCTCTTGATGCACATCTTGACCATTATGATTCTATAACCACAGGTCATATTAGCCAGGCACTTCATTATCTTAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGG‑‑tttttttt                                                                                                                            <  1:399682/140‑2 (MQ=255)
  aTGCTCTCCGGTGCTCTTGATGCACATCTTGACCATTATGATTCTATAACCACAGGTCATATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGG‑‑ttttttttg                                                                                                                           <  2:846723/140‑3 (MQ=255)
  aTGCTCTCCGGTGCTCTTGATGCACATCTTGACCATTATGATTCTATAACCACAGGTCATATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGG‑‑ttttttttg                                                                                                                           >  2:292969/1‑138 (MQ=255)
  aTGCTCTCCGGTGCTCTTGATGCACATCTTGACCATTATGATTCTATAACCACAGGTCATATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGG‑‑ttttttttg                                                                                                                           <  1:451092/140‑3 (MQ=255)
        tcCGGTGCTCTTGATGCACATCTTGACCATTATGATTCTATAACCACAGGTCATATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTa                                                                                                                      >  2:683610/1‑140 (MQ=255)
              gCTCTTGATGCACATCTTGACCATTATGATTCTATAACCACAGGTCATATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCa                                                                                                                <  2:627778/140‑1 (MQ=255)
               ctctTGATGCACATCTTGACCATTATGATTCTATAACCACAGGTCATATTAGCCAGGTACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGG‑‑TTTTTTTGGTCTTATTGGCATc                                                                                                              <  2:469426/140‑1 (MQ=255)
                tctTGATGCACATCTTGACCATTATGATTCTATAACCACAGGTCATATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGATCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATc                                                                                                              <  2:829649/140‑1 (MQ=255)
                tctTGATGCACATCTTCACCATTATGATTCTATAACCACAGGTCATATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATc                                                                                                              <  1:779141/140‑1 (MQ=255)
                  ttGATGCACATCTTGACCATTATGATTCTATAACCACAGGTCATATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCt                                                                                                            >  2:293740/1‑140 (MQ=255)
                    gATGCACATCTTGACCATTATGATTCTATAACCACAGGTCATATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGt                                                                                                          <  2:374675/140‑1 (MQ=255)
                       gCACATCTTGACCATTATGATTCTATAACCACAGGTCATATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTgc                                                                                                       >  2:585574/1‑140 (MQ=255)
                         acaTCTTGACCATTATGATTCTATAACCACAGGTCATATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTgcag                                                                                                     >  2:110464/1‑140 (MQ=255)
                           aTCTTGACCATTATGATTCTATAACCACAGGTCATATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTgcagca                                                                                                   <  1:335826/140‑1 (MQ=255)
                           aTCTTGACCATTATGATTCTATAACCACAGGTCATATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTgcagca                                                                                                   >  1:399907/1‑140 (MQ=255)
                              ttGACCATTATGATTCTATAACCACAGGTCATATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGc                                                                                                <  1:592825/140‑1 (MQ=255)
                                 aCCATTATGATTCTATAACCACAGGTCACATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTg                                                                                             >  2:688276/1‑140 (MQ=255)
                                  ccATTATGATTCTATAACCACAGGTCATATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGc                                                                                            >  2:249235/1‑140 (MQ=255)
                                     ttATGATTCTATAACCACAGGTCATATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGcatc                                                                                         >  2:330628/1‑140 (MQ=255)
                                      tATGATTCTATAACCACAGGTCATATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGcatca                                                                                        <  1:110464/140‑1 (MQ=255)
                                      tATGATTCTATAACCACAGGTCATATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGcatca                                                                                        <  1:683610/140‑1 (MQ=255)
                                       aTGATTCTATAACCACAGGTCATATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGcatca                                                                                        <  1:742740/139‑1 (MQ=255)
                                       aTGATTCTATAACCACAGGTCATATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGcatca                                                                                        >  2:742740/1‑139 (MQ=255)
                                            tCTATAACCACAGGTCATATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGcatca                                                                                        >  2:541613/1‑134 (MQ=255)
                                            tCTATAACCACAGGTCATATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGcatca                                                                                        <  1:541613/134‑1 (MQ=255)
                                             cTATAACCACAGGTCATATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTg                                                                                 <  2:331813/140‑1 (MQ=255)
                                             cTATAACCACAGGTCATATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTg                                                                                 <  1:73243/140‑1 (MQ=255)
                                                       aGGTCATATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATcacca                                                                       >  2:340379/1‑140 (MQ=255)
                                                        ggTCATATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGcatcat                                                                                       <  1:324897/123‑1 (MQ=255)
                                                        ggTCATATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGcatcat                                                                                       >  2:324897/1‑123 (MQ=255)
                                                        ggTCATATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCGTCATGGTCTGGCAATcaccac                                                                      >  2:789373/1‑140 (MQ=255)
                                                        ggTCATATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATcaccac                                                                      >  1:277289/1‑140 (MQ=255)
                                                        ggTCATATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATcaccac                                                                      >  2:772887/1‑140 (MQ=255)
                                                        ggTCATATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATcaccac                                                                      >  2:825932/1‑140 (MQ=255)
                                                            atatTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTc                                                                  >  1:734441/1‑140 (MQ=255)
                                                              atTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGa                                                                >  1:769003/1‑140 (MQ=255)
                                                                   ccAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTg                                                           >  2:369065/1‑140 (MQ=255)
                                                                   ccAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTg                                                           >  2:19055/1‑140 (MQ=255)
                                                                   ccAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTg                                                           >  2:183215/1‑140 (MQ=255)
                                                                   ccAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTg                                                           >  2:840317/1‑140 (MQ=255)
                                                                   ccAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTg                                                           >  2:166560/1‑140 (MQ=255)
                                                                       gCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCg                                                       >  2:677223/1‑140 (MQ=255)
                                                                       gCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCg                                                       >  2:337066/1‑140 (MQ=255)
                                                                       gCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCg                                                       >  1:340018/1‑140 (MQ=255)
                                                                       gCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCg                                                       >  2:660505/1‑140 (MQ=255)
                                                                       gCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCg                                                       >  1:503853/1‑140 (MQ=255)
                                                                       gCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCAAGGTCTGGCAATCACCACTGTCGAACCTGTTCg                                                       <  1:517553/140‑1 (MQ=255)
                                                                          cTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTgg                                                    <  1:677223/140‑1 (MQ=255)
                                                                              aTTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTcc                                                <  1:789373/140‑1 (MQ=255)
                                                                              aTTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTcc                                                <  1:249235/140‑1 (MQ=255)
                                                                                tATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTg                                              <  1:292969/140‑1 (MQ=255)
                                                                                tATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTg                                              <  1:166560/140‑1 (MQ=255)
                                                                                 aTCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGt                                             <  1:840317/140‑1 (MQ=255)
                                                                                 aTCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGt                                             <  1:660505/140‑1 (MQ=255)
                                                                                 aTCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGt                                             <  1:340379/140‑1 (MQ=255)
                                                                                 aTCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGt                                             <  1:369065/140‑1 (MQ=255)
                                                                                     cAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGt                                             >  2:284933/1‑136 (MQ=255)
                                                                                     cAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGt                                             <  1:284933/136‑1 (MQ=255)
                                                                                     cAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGt                                         >  1:686941/1‑140 (MQ=255)
                                                                                     cAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGt                                         >  1:39500/1‑140 (MQ=255)
                                                                                       atatTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGttt                                       >  2:30087/1‑140 (MQ=255)
                                                                                       atatTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGttt                                       >  2:365736/1‑140 (MQ=255)
                                                                                       atatTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGttt                                       >  1:175516/1‑140 (MQ=255)
                                                                                        tatTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTa                                      <  2:343056/140‑1 (MQ=255)
                                                                                        tatTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTa                                      >  1:73707/1‑140 (MQ=255)
                                                                                        tatTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTa                                      <  2:460005/140‑1 (MQ=255)
                                                                                           tGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTg                                   <  1:72880/140‑1 (MQ=255)
                                                                                            ggAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGTTTTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTg                                   >  1:687894/1‑140 (MQ=255)
                                                                                             gAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGtt                                 <  1:337066/140‑1 (MQ=255)
                                                                                             gAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGtt                                 >  1:435062/1‑140 (MQ=255)
                                                                                                gATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTAc                              <  2:318135/140‑1 (MQ=255)
                                                                                                  ttGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGtt                                        <  1:421269/128‑1 (MQ=255)
                                                                                                  ttGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGtt                                        >  2:421269/1‑128 (MQ=255)
                                                                                                  ttGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGa                            >  1:192009/1‑140 (MQ=255)
                                                                                                    gCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGatt                          >  2:509712/1‑140 (MQ=255)
                                                                                                     ccAGCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGatta                         >  1:433372/1‑140 (MQ=255)
                                                                                                        gCTCTCGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTg                      <  1:354120/140‑1 (MQ=255)
                                                                                                         ctctCGTCGTTCTCTGCTTACTGGCTGGTTTTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTg                      <  2:687894/140‑1 (MQ=255)
                                                                                                          tctcGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCg                    <  1:825932/140‑1 (MQ=255)
                                                                                                           ctcGTCGTTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCgg                   <  2:277289/140‑1 (MQ=255)
                                                                                                             cgtcgtTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTg                 <  2:686941/140‑1 (MQ=255)
                                                                                                               tcgtTCTCTGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCa               <  1:183215/140‑1 (MQ=255)
                                                                                                                      ctGCTTACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATAcc        >  1:589623/1‑140 (MQ=255)
                                                                                                                          ttACTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCCGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTa    >  1:168596/1‑140 (MQ=255)
                                                                                                                            aCTGGCTGGT‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTaaa  >  1:795868/1‑140 (MQ=255)
                                                                                                                                     |                                                                                                                                   
ACATGCTCTCCGGTGCTCTTGATGCACATCTTGACCATTATGATTCTATAACCACAGGTCATATTAGCCAGGCACTTCATTATCTCAATATTGGAAGATTGCCAGCTCTCGTCGTTCTCTGCTTACTGGCTGG‑‑TTTTTTTGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAA  >  NC_000913/3192305‑3192567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A