Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 139 58.0 1973578 98.9% 1951868 138.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,054,066 G→A E142K (GAA→AAA)  shiA → shikimate:H(+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,054,0660GA100.0% 212.8 / NA 60E142K (GAA→AAA) shiAshikimate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/35);  total (25/35)

ATGCTGACCGTCTGGATGATGGGCATCGCGACAGCCTTGATTGGTATTCTTCCTTCATTCTCGACCATTGGGTGGTGGGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCGAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCGGTGTACAAGTTGGCTACGGTGTAGGTTTACTGCTTTCAACCGGACTGGTTTCATTGATCAGT  >  NC_000913/2053934‑2054197
                                                                                                                                    |                                                                                                                                   
aTGCTGACCGTCTGGATGATGGGCATCGCGACAGCCTTGATTGGTATTCTTCCTTCATTCTCGACCATTGGGTGGTGGGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATgggg                                                                                                                              <  1:738266/140‑1 (MQ=255)
aTGCTGACCGTCTGGATGATGGGCATCGCGACAGCCTTGATTGGTATTCTTCCTTCATTCTCGACCATTGGGTGGTGGGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATgggg                                                                                                                              <  2:917639/140‑1 (MQ=255)
     gACCGTCTGGATGATGGGCATCGCGACAGCCTTGATTGGTATTCTTCCTTCATTCTCGACCATTGGGTGGTGGGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGgcg                                                                                                                         <  2:320464/140‑1 (MQ=255)
     gACCGTCTGGATGATGGGCATCGCGACAGCCTTGATTGGTATTCTTCCTTCATTCTCGACCATTGGGTGGTGGGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGgcg                                                                                                                         <  2:648901/140‑1 (MQ=255)
       ccGTCTGGATGATGGGCATCGCGACAGCCTTGATTGGTATTCTTCCTTCATTCTCGACCATTGGGTGGTGGGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGcgcg                                                                                                                       >  1:815423/1‑140 (MQ=255)
                        aTCGCGACAGCCTTGATTGGTATTCTTCCTTCATTCTCGACCATTGGGTGGTGGGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGa                                                                                                      >  1:827054/1‑140 (MQ=255)
                        aTCGCGACAGCCTTGATTGGTATTCTTCCTTCATTCTCGACCATTGGGTGGTGGGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGa                                                                                                      >  1:823005/1‑140 (MQ=255)
                        aTCGCGACAGCCTTGATTGGTATTCTTCCTTCATTCTCGACCATTGGGTGGTGGGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGa                                                                                                      <  1:13596/140‑1 (MQ=255)
                                  ccTTGATTGGTATTCTTCCTTCATTCTCGACCATTGGGTGGTGGGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGaaa                                                                                                    <  2:439163/132‑1 (MQ=255)
                                  ccTTGATTGGTATTCTTCCTTCATTCTCGACCATTGGGTGGTGGGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGaaa                                                                                                    >  1:439163/1‑132 (MQ=255)
                                             aTTCTTCCTTCATTCTCGACCATTGGGTGGTGGGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATaaaaa                                                                                 <  2:233892/140‑1 (MQ=255)
                                             aTTCTTCCTTCATTCTCGACCATTGGGTGGTGGGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATaaaaa                                                                                 >  1:303417/1‑140 (MQ=255)
                                                 ttccttcATTCTCGACCATTGGGTGGTGGGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGcc                                                                             >  2:273602/1‑140 (MQ=255)
                                                 ttccttcATTCTCGACCATTGGGTGGTGGGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGcc                                                                             <  2:815423/140‑1 (MQ=255)
                                                  tccttcATTCTCGACCATTGGGTGGTGGGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCt                                                                            <  2:303417/140‑1 (MQ=255)
                                                      tcATTCTCGACCATTGGGTGGTGGGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTa                                                                        >  1:229240/1‑140 (MQ=255)
                                                         ttCTCGACCATTGGGTGGTGGGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAg                                                                     >  2:697059/1‑140 (MQ=255)
                                                         ttCTCGACCATTGGGTGGTGGGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAg                                                                     <  2:108659/140‑1 (MQ=255)
                                                         ttCTCGACCATTGGGTGGTGGGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAg                                                                     <  1:292818/140‑1 (MQ=255)
                                                          tctcGACCATTGGGTGGTGGGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATaaaa                                                                                  <  1:672191/126‑1 (MQ=255)
                                                          tctcGACCATTGGGTGGTGGGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATaaaa                                                                                  >  2:672191/1‑126 (MQ=255)
                                                           ctcGACCATTGGGTGGTGGGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTa                                                                   >  2:234241/1‑140 (MQ=255)
                                                            tcGACCATTGGGTGGTGGGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAg                                                                  <  1:697059/140‑1 (MQ=255)
                                                            tcGACCATTGGGTGGTGGGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAg                                                                  <  1:234241/140‑1 (MQ=255)
                                                            tcGACCATTGGGTGGTGGGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAg                                                                  <  2:827054/140‑1 (MQ=255)
                                                              gACCATTGGGTGGTGGGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCg                                                                <  1:121604/140‑1 (MQ=255)
                                                              gACCATTGGGTGGTGGGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCg                                                                <  2:681804/140‑1 (MQ=255)
                                                                   ttGGGTGGTGGGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCGGTGTa                                                           <  2:703974/140‑1 (MQ=255)
                                                                    tGGGTGGTGGGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCGGTGTAc                                                          <  2:229240/140‑1 (MQ=255)
                                                                      ggtggtGGGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCGgtg                                                             <  1:960168/135‑1 (MQ=255)
                                                                      ggtggtGGGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCGgtg                                                             >  2:960168/1‑135 (MQ=255)
                                                                          gtggGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCGgtg                                                             <  2:601573/131‑1 (MQ=255)
                                                                          gtggGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCGgtg                                                             >  1:601573/1‑131 (MQ=255)
                                                                          gtggGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCGGTGTACAAGTTg                                                    >  2:673182/1‑140 (MQ=255)
                                                                          gtggGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCGGTGTACAAGTTg                                                    >  2:25878/1‑140 (MQ=255)
                                                                            gggCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCGGTGTACAAGTTGGc                                                  <  1:273602/140‑1 (MQ=255)
                                                                                  cTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCGGTGTACaa                                                        >  1:972235/1‑128 (MQ=255)
                                                                                  cTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCGGTGTACaa                                                        <  2:972235/128‑1 (MQ=255)
                                                                                  cTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCGGTGTACAAGTTGGc                                                  <  1:405235/134‑1 (MQ=255)
                                                                                  cTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCGGTGTACAAGTTGGc                                                  >  2:405235/1‑134 (MQ=255)
                                                                                     ttttGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCGGTGTACAAGTTGGCTACGGTGTa                                         >  1:616518/1‑140 (MQ=255)
                                                                                       ttGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCGGTGTACAAGTTGGCTACGGTGTAgg                                       >  2:124923/1‑140 (MQ=255)
                                                                                           tGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCGGTGTACAAGTTGGCTACGGTGTAGGTTTa                                   <  1:124923/140‑1 (MQ=255)
                                                                                            ggTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCGGTGTACAAGTTGGCTACGGTGTAGGTTTAc                                  >  2:826370/1‑140 (MQ=255)
                                                                                             gTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCGGTGTACAAGTTGGCTACGGTGTAGGTTTACt                                 >  2:205852/1‑140 (MQ=255)
                                                                                                acacTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCGGTGTACAAGTTGGCTACGGTGTAGGTTTACTGCt                              <  1:455698/140‑1 (MQ=255)
                                                                                                       gTGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCGGTGTACAAGTTGGCTACGGTGTAGGTTTACTGCTTTCAAcc                       >  2:268996/1‑140 (MQ=255)
                                                                                                        tGCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCGGTGTACAAGTTGGCTACGGTGTAGGTTTACTGCTTTCAACCg                      >  1:222594/1‑140 (MQ=255)
                                                                                                         gCCATTCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCGGTGTACAAGTTGGCTACGGTGTAGGTTTACTGCTTTCAACCgg                     <  2:616518/140‑1 (MQ=255)
                                                                                                              tCAGGGATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCGGTGTACAAGTTGGCTACGGTGTAGGTTTACTGCTTTCAACCGGACTgg                >  1:648847/1‑140 (MQ=255)
                                                                                                                   gATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCGGTGTACAAGTTGGCTACGGTGTAGGTTTACTGCTTTCAACCGGACTGGTTTCa           <  2:823005/140‑1 (MQ=255)
                                                                                                                   gATTTGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCGGTGTACAAGTTGGCTACGGTGTAGGTTTACTGCTTTCAACCGGACTGGTTTCa           <  2:222594/140‑1 (MQ=255)
                                                                                                                      ttGCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCGGTGTACAAGTTGGCTACGGTGTAGGTTTACTGCTTTCAACCGGACTGGTTTCATTg        <  1:826370/140‑1 (MQ=255)
                                                                                                                        gCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCGGTGTACAAGTTGGCTACGGTGTAGGTTTACTGCTTTCAACCGGACTGGTTTCATTGAt      <  1:673182/140‑1 (MQ=255)
                                                                                                                        gCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCGGTGTACAAGTTGGCTACGGTGTAGGTTTACTGCTTTCAACCGGACTGGTTTCATTGAt      <  1:268996/140‑1 (MQ=255)
                                                                                                                        gCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCGGTGTACAAGTTGGCTACGGTGTAGGTTTACTGCTTTCAACCGGACTGGTTTCATTGAt      <  1:25878/140‑1 (MQ=255)
                                                                                                                        gCAGTCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCGGTGTACAAGTTGGCTACGGTGTAGGTTTACTGCTTTCAACCGGACTGGTTTCATTGAt      <  1:205852/140‑1 (MQ=255)
                                                                                                                            tCGGCGGCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCGGTGTACAAGTTGGCTACGGTGTAGGTTTACTGCTTTCAACCGGACTGGTTTCATTGATCAGt  >  2:240405/1‑140 (MQ=255)
                                                                                                                             cggcggCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCGGTGTACAAGTTGGCTACGGTGTAGGTTTACTGCTTTCAACCGGACTGGTTTCATTGAt      >  1:904726/1‑135 (MQ=255)
                                                                                                                             cggcggCAAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCGGTGTACAAGTTGGCTACGGTGTAGGTTTACTGCTTTCAACCGGACTGGTTTCATTGAt      <  2:904726/135‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                   
ATGCTGACCGTCTGGATGATGGGCATCGCGACAGCCTTGATTGGTATTCTTCCTTCATTCTCGACCATTGGGTGGTGGGCACCTATTTTGCTGGTGACACTGCGTGCCATTCAGGGATTTGCAGTCGGCGGCGAATGGGGAGGCGCGGCGTTGCTTTCCGTTGAAAGTGCACCGAAAAATAAAAAAGCCTTTTACAGTAGCGGTGTACAAGTTGGCTACGGTGTAGGTTTACTGCTTTCAACCGGACTGGTTTCATTGATCAGT  >  NC_000913/2053934‑2054197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A