Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	139	58.0	1973578	98.9%	1951868	138.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,296,418	T→C	T203A (ACC→GCC)	ccmB ←	heme trafficking system membrane protein CcmB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,296,418	0	T	C	100.0%	143.1 / NA	43	T203A (ACC→GCC)	ccmB	heme trafficking system membrane protein CcmB
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/24); total (19/24)

GGATCGCCAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGTGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGCGGTAACACCAGTATGCTGAG  >  NC_000913/2296290‑2296544

ggATCGCCAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCa                                                                                                                     >  2:943682/1‑140 (MQ=255)

     gCCAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGcgcc                                                                                                                             <  1:215100/127‑1 (MQ=255)

     gCCAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGcgcc                                                                                                                             >  2:215100/1‑127 (MQ=255)

               gCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTg                                                                                                      <  2:713578/140‑1 (MQ=255)

               gCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTg                                                                                                      >  1:806224/1‑140 (MQ=255)

                    gtTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGTCAGCAACGCGCCTAAAATTGCCAGa                                                                                                 >  2:157486/1‑140 (MQ=255)

                    gtTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGa                                                                                                 <  2:883902/140‑1 (MQ=255)

                    gtTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGa                                                                                                 <  1:325879/140‑1 (MQ=255)

                    gtTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGa                                                                                                 >  1:910658/1‑140 (MQ=255)

                        tCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATAcc                                                                                             >  1:658747/1‑140 (MQ=255)

                             aTAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCa                                                                                        >  2:325123/1‑140 (MQ=255)

                                gTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATCCCCGTCAACg                                                                                     <  2:265669/140‑1 (MQ=255)

                                   tCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGc                                                                                  <  1:943682/140‑1 (MQ=255)

                                    cGATACCAGACTCGACCAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCTAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCa                                                                                 <  2:138656/140‑1 (MQ=255)

                                     gATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCaa                                                                                <  1:178356/140‑1 (MQ=255)

                                     gATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCaa                                                                                <  1:606430/140‑1 (MQ=255)

                                      aTACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCaaa                                                                               <  1:590422/140‑1 (MQ=255)

                                                 gAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGc                                                                    >  2:941988/1‑140 (MQ=255)

                                                 gAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGc                                                                    >  2:2986/1‑140 (MQ=255)

                                                 gAACAAAAACCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGc                                                                    >  2:593117/1‑140 (MQ=255)

                                                  aaCAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCaaa                                                                               <  1:70429/128‑1 (MQ=255)

                                                  aaCAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCaaa                                                                               >  2:70429/1‑128 (MQ=255)

                                                  aaCAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGcc                                                                   >  2:459686/1‑140 (MQ=255)

                                                  aaCAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGcc                                                                   >  2:510648/1‑140 (MQ=255)

                                                                  ccAGCGTTATTGAATGCTGATTCGTACCGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGcggcgg                                                   <  1:230938/140‑1 (MQ=255)

                                                                       gTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCa                                              <  2:910658/140‑1 (MQ=255)

                                                                          aTTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAg                                           <  1:325123/140‑1 (MQ=255)

                                                                            tcaaTGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGAt                                         <  2:580603/138‑1 (MQ=255)

                                                                            tGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGAt                                         <  1:510648/140‑1 (MQ=255)

                                                                            tGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGAt                                         <  1:672413/140‑1 (MQ=255)

                                                                                tGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGt                                     >  1:979104/1‑140 (MQ=255)

                                                                                tGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGt                                     >  1:12871/1‑140 (MQ=255)

                                                                                      ttCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATggg                               <  1:799436/140‑1 (MQ=255)

                                                                                      ttCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCATCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATggg                               <  2:365451/140‑1 (MQ=255)

                                                                                          tAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAg                           >  2:234191/1‑140 (MQ=255)

                                                                                            aCGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTc                         <  2:979104/140‑1 (MQ=255)

                                                                                             cGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCAGTGGCAAAGATGAGTAATGGGATAGTCa                        <  2:658747/140‑1 (MQ=255)

                                                                                                  ccgccgTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGc                      <  1:283640/137‑1 (MQ=255)

                                                                                                  ccgccgTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGc                      >  2:283640/1‑137 (MQ=255)

                                                                                                      cgTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGCGGTAaca               <  1:2986/140‑1 (MQ=255)

                                                                                                            aaaaGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGCgg                    <  2:723274/129‑1 (MQ=255)

                                                                                                            aaaaGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGCgg                    >  1:723274/1‑129 (MQ=255)

                                                                                                                   cTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGCGGTAACACCAGTATGCTGag  <  2:225649/140‑1 (MQ=255)

                                                                                                                   cTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGCGGTAACACCAGTATGCTGag  >  1:912442/1‑140 (MQ=255)

                                                                                                                        tGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGCGGTAACACCAGTATGCTGag  >  2:230802/1‑135 (MQ=255)

                                                                                                                        tGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGCGGTAACACCAGTATGCTGag  <  1:230802/135‑1 (MQ=255)

GGATCGCCAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGTGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGCGGTAACACCAGTATGCTGAG  >  NC_000913/2296290‑2296544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A