Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	139	58.0	1973578	98.9%	1951868	138.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,619,024	G→A	G379G (GGC→GGT)	uraA ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,619,024	0	G	A	100.0%	173.4 / NA	50	G379G (GGC→GGT)	uraA	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (28/22); total (28/22)

CGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGC  >  NC_000913/2618900‑2619154

cGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCgatg                                                                                                                     <  2:447803/140‑1 (MQ=255)

cGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCgatg                                                                                                                     >  1:667893/1‑140 (MQ=255)

    cAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCa                                                                                                                 >  1:599486/1‑140 (MQ=255)

     aGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCaa                                                                                                                <  2:769273/140‑1 (MQ=255)

          cacTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCa                                                                                                           >  2:501532/1‑140 (MQ=255)

            cttcttCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACg                                                                                                         >  1:923695/1‑140 (MQ=255)

            cttcttCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACg                                                                                                         <  1:422660/140‑1 (MQ=255)

             ttcttcAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACgg                                                                                                        <  1:581433/140‑1 (MQ=255)

               cttcAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGaa                                                                                                      >  2:260903/1‑140 (MQ=255)

                 tcAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGt                                                                                                    >  2:379922/1‑140 (MQ=255)

                    gggCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAg                                                                                                 >  2:616523/1‑140 (MQ=255)

                       cgcAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGAt                                                                                              >  2:820944/1‑140 (MQ=255)

                                    tCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCt                                                                                 <  2:667893/140‑1 (MQ=255)

                                    tCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCt                                                                                 <  1:319116/140‑1 (MQ=255)

                                     cAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCtt                                                                                <  1:210959/140‑1 (MQ=255)

                                     cAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCtt                                                                                <  2:599486/140‑1 (MQ=255)

                                        tttGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTttgt                                                                             >  1:90184/1‑140 (MQ=255)

                                          tGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTttgttg                                                                           <  1:820944/140‑1 (MQ=255)

                                             aGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTaa                                                                        >  1:510520/1‑140 (MQ=255)

                                                  aaaCTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCtt                                                                                <  1:470611/127‑1 (MQ=255)

                                                  aaaCTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCtt                                                                                >  2:470611/1‑127 (MQ=255)

                                                  aaaCTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCAc                                                                   >  1:702857/1‑140 (MQ=255)

                                                              aTACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTtcgattcgat                                                       >  1:585097/1‑140 (MQ=255)

                                                                aCCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATc                                                                      >  1:348039/1‑123 (MQ=255)

                                                                aCCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATc                                                                      <  2:348039/123‑1 (MQ=255)

                                                                    acgaTGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAac                                                 >  1:926815/1‑140 (MQ=255)

                                                                    acgaTGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAac                                                 >  2:713926/1‑140 (MQ=255)

                                                                      gaTGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAacac                                               <  1:379922/140‑1 (MQ=255)

                                                                         ggTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGaa                                            >  2:235171/1‑140 (MQ=255)

                                                                               cAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGa                                             >  1:397818/1‑133 (MQ=255)

                                                                               cAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGa                                             <  2:397818/133‑1 (MQ=255)

                                                                               cAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCgg                                      <  2:923695/140‑1 (MQ=255)

                                                                                aGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGa                                     <  1:235171/140‑1 (MQ=255)

                                                                                 gcgcCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGaa                                    <  2:510520/140‑1 (MQ=255)

                                                                                       tACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGaa                                    >  2:543122/1‑134 (MQ=255)

                                                                                       tACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGaa                                    <  1:543122/134‑1 (MQ=255)

                                                                                         ccTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAg                                   <  2:431434/133‑1 (MQ=255)

                                                                                         ccTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAg                                   >  1:431434/1‑133 (MQ=255)

                                                                                         ccTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGAt                            <  2:90184/140‑1 (MQ=255)

                                                                                         ccTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGAt                            <  2:713011/140‑1 (MQ=255)

                                                                                               aaCTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACAcc                      >  2:293319/1‑140 (MQ=255)

                                                                                                   ccgccgCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATaa                  >  2:226213/1‑140 (MQ=255)

                                                                                                    cgccgcgccgATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATaaa                 >  1:4914/1‑140 (MQ=255)

                                                                                                    cgccgcgccgATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATaaa                 >  2:741641/1‑140 (MQ=255)

                                                                                                    cgccgcgccgATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATaaa                 >  1:433941/1‑140 (MQ=255)

                                                                                                    cgccgcgccgATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATaaa                 >  2:317331/1‑140 (MQ=255)

                                                                                                      ccgcgccgATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATAAagc               >  1:749617/1‑140 (MQ=255)

                                                                                                      ccgcgccgATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATAAagc               >  1:19625/1‑140 (MQ=255)

                                                                                                              aTGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGa       <  1:681460/140‑1 (MQ=255)

                                                                                                                gTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGaaa     <  2:702857/140‑1 (MQ=255)

                                                                                                                   tACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAAcgc  <  2:690533/140‑1 (MQ=255)

CGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGC  >  NC_000913/2618900‑2619154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A