Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R1
|
139 |
58.0 |
1973578 |
98.9% |
1951868 |
138.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,694,252 |
T→C |
N431S (AAC→AGC) |
purL ← |
phosphoribosylformylglycinamide synthetase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,694,252 | 0 | T | C | 100.0%
| 60.8
/ NA
| 18 | N431S (AAC→AGC) | purL | phosphoribosylformylglycinamide synthetase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (14/4); total (14/4) |
GTTGTCGCGCTGTACGGAAGCAAAGTCGAGGTCGGCATCAGACTGACCAGACGCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTT > NC_000913/2694122‑2694388
|
gTTGTCGCGCTGTACGGAAGCAAAGTCGAGGTCGGCATCAGACTGACCAGACGCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGCTGATCTCGc < 1:732628/140‑1 (MQ=255)
gCAAAGTCGAGGTCGGCATCAGACTGACCAGACGCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGgc < 2:663001/140‑1 (MQ=255)
cAAAGTCGAGGTCGGCATCAGACTGACCAGACGCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGgcg < 1:235996/140‑1 (MQ=255)
cAAAGTCGAGGTCGGCATCAGACTGACCAGACGCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGgcg > 2:588863/1‑140 (MQ=255)
aCGCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATg > 1:321409/1‑140 (MQ=255)
aCGCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATg > 2:956776/1‑140 (MQ=255)
aTAGAAGCCCCTGCCCCCCCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGTCGATCCCGCCCGCCAGCATGATCgg < 2:372348/140‑1 (MQ=255)
gCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGTCGATCCCGCCCGCCAGCATGATCGGTTTGTGATa > 1:372348/1‑140 (MQ=255)
gCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATa > 2:372403/1‑140 (MQ=255)
caccacCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACcg > 1:585382/1‑140 (MQ=255)
ccAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGctc > 1:283642/1‑140 (MQ=255)
aaGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCtt > 2:868387/1‑140 (MQ=255)
tGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGt > 1:903263/1‑140 (MQ=255)
tGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGt > 1:508837/1‑140 (MQ=255)
ggCCGCCGAGAACGACCAGCTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACt > 1:565239/1‑140 (MQ=255)
ggCCGCCGAGAACGACCAGCTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACt > 2:932575/1‑140 (MQ=255)
ccgAGACCGCCCAGCTCCCCCCCGACGCTGTCCTCCCTTTTTTGTACGTGATCGGCGCAAATGTTGCCGCCCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTttc < 2:758137/140‑1 (MQ=255)
ccgAGAACGACCAGCTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTttc > 1:758137/1‑140 (MQ=255)
aaCGCCCAGCTTCGCCCCGACGCTGTTCTCCCTTTTTTGTCCGTGATCGGCGCGATTGTTGCCGCCCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCAt < 2:565239/140‑1 (MQ=255)
aaCGACCAGCTTCGCCCCGACGCTGATCTCGCTTTTTTGTACGTGATCGGCGCGATTGTTCCCGACCCCCCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCAt < 2:766394/140‑1 (MQ=255)
aaCGACCAGCTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCAt < 1:372403/140‑1 (MQ=255)
aaCGACCACCTCCCCCCCGACGCTGATCTCGCCTTTTTGTACGTGAGCGGCGCGAATGTTGCCGCCCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCAt < 2:283642/140‑1 (MQ=255)
ccAGCTTCGCACCGACGCTGATCTCGCTTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTa < 2:178314/140‑1 (MQ=255)
cTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGa > 1:766394/1‑140 (MQ=255)
ccccGACGTTGTTCTCGCTTTTTTGTCCGTGATGGGCGCGATTGTCGCCGACCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAg < 2:903263/138‑1 (MQ=255)
cACCGACGCTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAg < 1:868387/140‑1 (MQ=255)
gcGTTGTTCTCCTTTTTTTTTGCGTGTCGGGGGCGATTGTGGCGGACCCCACCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGtt < 2:144156/139‑1 (MQ=255)
|
GTTGTCGCGCTGTACGGAAGCAAAGTCGAGGTCGGCATCAGACTGACCAGACGCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTT > NC_000913/2694122‑2694388
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
