Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	139	58.0	1973578	98.9%	1951868	138.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,712,093	A→G	R224R (CGT→CGC)	yfiC ←	tRNA m(6)A37 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,712,093	0	A	G	100.0%	118.6 / NA	34	R224R (CGT→CGC)	yfiC	tRNA m(6)A37 methyltransferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (21/13); total (21/13)

GCATTTCACGCTGGATTATCCGGAACTGCTCACCCATTCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGC  >  NC_000913/2711958‑2712228

gCATTTCACGCTGGATTATCCGGAACTGCTCACCCATTCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCGCGaa                                                                                                                                     <  2:354809/140‑1 (MQ=255)

        cGCTGGATTATCCGGAACTGCTCACCCATTCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCGCGAATGACTAAg                                                                                                                             >  2:728452/1‑140 (MQ=255)

          cTGGATTATCCGGAACTGCTCACCCATTCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCGCGAATGACTAAGCg                                                                                                                           <  1:728452/140‑1 (MQ=255)

                 aTCCGGAACTGCTCACCCATTCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCGCGAATGACTAAGCGATCGCTa                                                                                                                    <  1:622288/140‑1 (MQ=255)

                   ccGGAACTGCTCACCCATTCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCGCGAATGACTAAGCGATCGCTaaa                                                                                                                  >  1:475570/1‑140 (MQ=255)

                            cTCACCCATTCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCGCGAATGACTAAGCGATCGCTAAAGCATTCTcc                                                                                                         >  2:117820/1‑140 (MQ=255)

                            cTCACCCATTCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCGCGAATGACTAAGCGATCGCTAAAGCATTCTcc                                                                                                         <  1:290377/140‑1 (MQ=255)

                            cTCACCCATTCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCGCGAATGACTAAGCGATCGCTAAAGCATTCTcc                                                                                                         >  2:290377/1‑140 (MQ=255)

                            cTCACCCATTCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCGCGAATGACTAAGCGATCGCTAAAGCATTCTcc                                                                                                         >  2:177906/1‑140 (MQ=255)

                            cTCACCCATTCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCGCGAATGACTAAGCGATCGCTAAAGCATTCTcc                                                                                                         >  2:550032/1‑140 (MQ=255)

                               ccccATTCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCGCGAATGACTAAGCGATCGCTAAAGCATTCTCCCGc                                                                                                      <  1:117820/139‑1 (MQ=255)

                                 ccATTCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCGCGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCt                                                                                                    >  1:388311/1‑140 (MQ=255)

                                         gTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCGCGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCg                                                                                                 >  1:612750/1‑135 (MQ=255)

                                         gTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCGCGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCg                                                                                                 <  2:612750/135‑1 (MQ=255)

                                            cGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCGCGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAAt                                                                                         <  1:550032/140‑1 (MQ=255)

                                               cGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCGCGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGcc                                                                                      >  1:617606/1‑140 (MQ=255)

                                                      ttCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCGCGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCGTGCg                                                                                                 >  1:469432/1‑122 (MQ=255)

                                                      ttCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCGCGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCGTGCg                                                                                                 <  2:469432/122‑1 (MQ=255)

                                                                     ttACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCGCGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTc                                                                >  2:149309/1‑140 (MQ=255)

                                                                             aCAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCGCGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCg                                                        >  1:647859/1‑140 (MQ=255)

                                                                                gATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCGCGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGttt                                                     <  2:36839/140‑1 (MQ=255)

                                                                                gATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCGCGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGttt                                                     >  1:737493/1‑140 (MQ=255)

                                                                                gATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCGCGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGttt                                                     >  2:840953/1‑140 (MQ=255)

                                                                                    aaaaGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCGCGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCg                                                 <  1:149309/140‑1 (MQ=255)

                                                                                                  gcgcCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCGCGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGc                                   >  1:274731/1‑140 (MQ=255)

                                                                                                   cgcCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCGCGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCa                                  >  1:808665/1‑140 (MQ=255)

                                                                                                        tataCGCTTCGGAATAGTTCTGGTCTGGCCCGCGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGt                             <  1:840953/140‑1 (MQ=255)

                                                                                                                  ggAATAGTTCTGGTCTGGCCCGCGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGc                   >  2:475684/1‑140 (MQ=255)

                                                                                                                  ggAATAGTTCTGGTCTGGCCCGCGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGc                   >  1:462919/1‑140 (MQ=255)

                                                                                                                    aaTAGTTCTGGTCTGGCCCGCAAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGccc                 <  2:412526/140‑1 (MQ=255)

                                                                                                                     aTAGTTCTGGTCTGGCCCGCGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCa                <  2:351573/140‑1 (MQ=255)

                                                                                                                                   gCCCGCGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGcc                  >  1:911637/1‑124 (MQ=255)

                                                                                                                                   gCCCGCGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGcc                  <  2:911637/124‑1 (MQ=255)

                                                                                                                                   gCCCGCGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGc  >  2:242748/1‑140 (MQ=255)

                                                                                                                                   gCCCGCGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGc  >  1:382631/1‑140 (MQ=255)

GCATTTCACGCTGGATTATCCGGAACTGCTCACCCATTCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGC  >  NC_000913/2711958‑2712228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A