Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	139	58.0	1973578	98.9%	1951868	138.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,236,458	A→G	I167V (ATC→GTC)	ygjP →	putative metal‑dependent hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,236,458	0	A	G	100.0%	192.6 / NA	53	I167V (ATC→GTC)	ygjP	putative metal‑dependent hydrolase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (28/25); total (28/25)

CGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAATCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGA  >  NC_000913/3236322‑3236591

cGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCt                                                                                                                                    >  1:362314/1‑140 (MQ=255)

 gAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTg                                                                                                                                   >  2:746484/1‑140 (MQ=255)

      ggCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCt                                                                                                                                    >  2:357808/1‑134 (MQ=255)

      ggCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCt                                                                                                                                    <  1:357808/134‑1 (MQ=255)

      ggCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAat                                                                                                                              >  2:660195/1‑140 (MQ=255)

      ggCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAat                                                                                                                              <  1:746484/140‑1 (MQ=255)

             cTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCa                                                                                                                       >  2:706627/1‑140 (MQ=255)

                       aaGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTAtct                                                                                                             >  2:543776/1‑140 (MQ=255)

                          agCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAATCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGc                                                                                                                    >  1:299007/1‑130 (MQ=255)

                          agCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAATCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGc                                                                                                                    <  2:299007/130‑1 (MQ=255)

                            cATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTAtcttttct                                                                                                        <  1:660195/140‑1 (MQ=255)

                                aCAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTAt                                                                                                               >  2:356592/1‑129 (MQ=255)

                                aCAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTAt                                                                                                               <  1:356592/129‑1 (MQ=255)

                                    aGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAg                                                                                                >  2:63578/1‑140 (MQ=255)

                                                  tgtgttgtCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGAc                                                                                  <  1:45255/140‑1 (MQ=255)

                                                  tgtgttgtCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGAc                                                                                  <  1:370980/140‑1 (MQ=255)

                                                  tgtgttgtCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGAc                                                                                  <  1:706627/140‑1 (MQ=255)

                                                      ttgtCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTcc                                                                              <  2:463899/140‑1 (MQ=255)

                                                      ttgtCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTcc                                                                              >  2:517383/1‑140 (MQ=255)

                                                       tgtCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTccc                                                                             <  2:931350/140‑1 (MQ=255)

                                                       tgtCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTccc                                                                             <  2:389715/140‑1 (MQ=255)

                                                            tatGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCtt                                                                        <  1:575824/140‑1 (MQ=255)

                                                            tatGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCtt                                                                        <  2:362314/140‑1 (MQ=255)

                                                                gAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGt                                                                    >  1:543128/1‑140 (MQ=255)

                                                                   ccccAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTaa                                                                 >  2:296682/1‑140 (MQ=255)

                                                                   ccccAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTaa                                                                 >  1:315969/1‑140 (MQ=255)

                                                                         tACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATaa                                                           <  1:63578/140‑1 (MQ=255)

                                                                         tACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATaa                                                           <  2:315969/140‑1 (MQ=255)

                                                                           ccaccaGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACg                                                         >  2:804606/1‑140 (MQ=255)

                                                                           ccaccaGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACg                                                         >  1:351846/1‑140 (MQ=255)

                                                                              ccaGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCtt                                                                        >  1:715360/1‑122 (MQ=255)

                                                                              ccaGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCtt                                                                        <  2:715360/122‑1 (MQ=255)

                                                                              ccaGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGAcc                                                      >  1:668168/1‑140 (MQ=255)

                                                                                 gCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCAtg                                                   >  1:944816/1‑140 (MQ=255)

                                                                                     gAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCAtgctgc                                               <  1:517383/140‑1 (MQ=255)

                                                                                     gAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCAtgctgc                                               <  2:543128/140‑1 (MQ=255)

                                                                                         tCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTgcgcgc                                           >  1:518829/1‑140 (MQ=255)

                                                                                           gACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCAt                                         >  2:867919/1‑140 (MQ=255)

                                                                                               cccGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGc                                     <  2:351846/140‑1 (MQ=255)

                                                                                                       tgGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCcttc                             >  2:398071/1‑140 (MQ=255)

                                                                                                        ggCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCcttct                            >  1:198993/1‑140 (MQ=255)

                                                                                                         gCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCcttctt                           >  2:462652/1‑140 (MQ=255)

                                                                                                              cGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCg                      <  2:944816/140‑1 (MQ=255)

                                                                                                                 aGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTAt                   >  1:6309/1‑140 (MQ=255)

                                                                                                                        gCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGtttt            >  2:489088/1‑140 (MQ=255)

                                                                                                                             ggCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCg       <  2:380186/140‑1 (MQ=255)

                                                                                                                             ggCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCg       >  1:380186/1‑140 (MQ=255)

                                                                                                                                aGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCg    <  1:489088/140‑1 (MQ=255)

                                                                                                                                 gAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCgg   <  2:198993/140‑1 (MQ=255)

                                                                                                                                 gAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCgg   <  1:398071/140‑1 (MQ=255)

                                                                                                                                  aaCAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCg    >  1:885420/1‑138 (MQ=255)

                                                                                                                                  aaCAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCg    <  2:885420/138‑1 (MQ=255)

                                                                                                                                  aaCAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGa  >  1:466040/1‑140 (MQ=255)

CGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAATCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGA  >  NC_000913/3236322‑3236591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A