Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	176	31.5	1082462	98.8%	1069472	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,804,622	T→C	pseudogene (1529/1900 nt)	arpB →	putative ankyrin repeat protein B, N‑terminal fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,804,622	0	T	C	100.0%	145.1 / NA	40	pseudogene (1529/1900 nt)	arpB	putative ankyrin repeat protein B, N‑terminal fragment
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (14/26); total (14/26)

GAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAA  >  NC_000913/1804495‑1804752

gAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACGGt                                                                                                                        >  2:445261/1‑140 (MQ=255)

               tCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACGGTTCct                                                                                                                    >  2:412882/1‑129 (MQ=255)

               tCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACGGTTCct                                                                                                                    <  1:412882/129‑1 (MQ=255)

               tCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTa                                                                                                         >  1:118650/1‑140 (MQ=255)

                    tAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTAATGGc                                                                                                    >  2:530796/1‑140 (MQ=255)

                      gCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTAATGGCAt                                                                                                  <  1:445261/140‑1 (MQ=255)

                      gCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTAATGGCAt                                                                                                  <  2:118650/140‑1 (MQ=255)

                      gCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTAATGGCAt                                                                                                  <  1:58700/140‑1 (MQ=255)

                         caAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTAATGGCAt                                                                                                  <  1:365510/137‑1 (MQ=255)

                         caAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTAATGGCAt                                                                                                  >  2:365510/1‑137 (MQ=255)

                         caAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCg                                                                                                >  1:215398/1‑139 (MQ=255)

                         caAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCg                                                                                                <  2:215398/139‑1 (MQ=255)

                         caAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGc                                                                                               >  2:531580/1‑140 (MQ=255)

                               cTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTaa                                                                                         >  1:408860/1‑140 (MQ=255)

                                ttttAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTaaa                                                                                        <  2:140834/140‑1 (MQ=255)

                                 tttAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAAt                                                                                       <  1:530796/140‑1 (MQ=255)

                                  ttAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATa                                                                                      <  1:391104/140‑1 (MQ=255)

                                                  tgatgaGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCt                                                                      <  2:140462/140‑1 (MQ=255)

                                                  tgatgaGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCt                                                                      <  2:533033/140‑1 (MQ=255)

                                                            tttAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATgg                                                            >  1:335820/1‑140 (MQ=255)

                                                              tAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGAt                                                          <  1:58232/140‑1 (MQ=255)

                                                                 ccATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATtta                                                       >  1:53849/1‑140 (MQ=255)

                                                                 ccATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATtta                                                       >  2:81090/1‑140 (MQ=255)

                                                                   aTGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATttatt                                                     >  2:494514/1‑140 (MQ=255)

                                                                    tGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATttatta                                                    <  2:310556/140‑1 (MQ=255)

                                                                         aTACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAgg                                               <  2:533253/140‑1 (MQ=255)

                                                                         aTACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAgg                                               <  2:378135/140‑1 (MQ=255)

                                                                         aTACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAgg                                               <  2:225525/140‑1 (MQ=255)

                                                                         aTACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAgg                                               <  2:58312/140‑1 (MQ=255)

                                                                              tgatgGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTa                                          <  1:531580/140‑1 (MQ=255)

                                                                               gatgGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTAc                                         >  1:222911/1‑140 (MQ=255)

                                                                                        ccAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAggg                                <  2:521796/140‑1 (MQ=255)

                                                                                             aTTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAAccc                           <  2:94326/140‑1 (MQ=255)

                                                                                             aTTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAAccc                           <  2:265514/140‑1 (MQ=255)

                                                                                             aTTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAAccc                           <  1:308767/140‑1 (MQ=255)

                                                                                             aTTATACGCCGCAATGGAAAATAATCACCCTTTGCGTGTCACACAGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAAccc                           <  2:348835/140‑1 (MQ=255)

                                                                                                      cgcAATGGAAAATAATCACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATtat                  >  2:373436/1‑140 (MQ=255)

                                                                                                         aaTGGAAAATAATCACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACa               <  2:335820/140‑1 (MQ=255)

                                                                                                                     tCACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCa   <  2:222911/140‑1 (MQ=255)

                                                                                                                      cACCCTTTGCGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCaa  <  1:494514/140‑1 (MQ=255)

GAATGCATTAACCACTCCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAA  >  NC_000913/1804495‑1804752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A