Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F28 I1 R1
|
176 |
31.5 |
1082462 |
98.8% |
1069472 |
138.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,891,751 |
(C)5→6 |
coding (1485/1911 nt) |
yoaA ← |
putative 5' to 3' DNA helicase implicated in DNA repair |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,891,746 | 1 | . | C | 100.0%
| 65.6
/ NA
| 19 | E497G (GAA→GGA) | yoaA | putative 5' to 3' DNA helicase implicated in DNA repair |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (9/10); total (9/10) |
AAACGGCAATTTGTCGATAATTACCAATGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTT‑CCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACA > NC_000913/1891610‑1891825
|
aaaCGGCAATTTGTCGATAATTACCAATGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTT‑ccc < 2:160223/140‑1 (MQ=255)
cGGCAATTTGTCGATAATTACCAATGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTT‑cccccc < 2:244570/140‑2 (MQ=255)
gCAATTTGTCGATAATTACCAATGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTT‑cccccctg < 2:35321/140‑4 (MQ=255)
tGTCGATAATTACCAATGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGcaacaa < 1:64589/140‑1 (MQ=255)
aTAATTACCAATGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGCAACAATACAg > 1:518863/1‑140 (MQ=255)
aaTTACCAATGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCGTTGCTGGTTTCCCCCCTGCAACAATACAGGa < 2:411044/140‑1 (MQ=255)
ttACCAATGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGCAACAATACAGGAAg < 1:326826/140‑1 (MQ=255)
aTGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGCAACAATACAGGAAGCGTCATGGTAgc > 1:457765/1‑140 (MQ=255)
cGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGaa > 2:170518/1‑140 (MQ=255)
cacgcacgTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTg < 2:75687/140‑1 (MQ=255)
cgcacgTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCt > 1:112686/1‑140 (MQ=255)
tCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCa < 2:112686/140‑1 (MQ=255)
cACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGa > 2:433729/1‑140 (MQ=255)
tCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGca > 1:393176/1‑140 (MQ=255)
tCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGca > 1:166894/1‑140 (MQ=255)
aaCTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGc < 2:10353/140‑1 (MQ=255)
ctgGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCg < 2:518863/140‑1 (MQ=255)
tGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTa > 2:185651/1‑140 (MQ=255)
ccACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACa < 1:185651/140‑1 (MQ=255)
ccACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACa < 2:433139/140‑1 (MQ=255)
cacaAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGt < 1:266391/129‑1 (MQ=255)
cacaAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGt > 2:266391/1‑129 (MQ=255)
|
AAACGGCAATTTGTCGATAATTACCAATGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTT‑CCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACA > NC_000913/1891610‑1891825
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
