Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F28 I1 R1 176 31.5 1082462 98.8% 1069472 138.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,949,990 A→G V178A (GTG→GCG)  aspS ← aspartate‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,949,9900AG100.0% 53.9 / NA 16V178A (GTG→GCG) aspSaspartate‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/5);  total (11/5)

AAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATCCATAAAACGGCGCACCAGGCTGGTGATTTTAGCGCGGGTTTTCAGGCGCTGAGC  >  NC_000913/1949876‑1950120
                                                                                                                  |                                                                                                                                  
aaGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCGCCAGGTAGTCACGCGCGCCTTCCgg                                                                                                           >  2:270942/1‑140 (MQ=255)
  gCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCGCCAGGTAGTCACGCGCGCCTTCCGGCg                                                                                                         <  1:168238/140‑1 (MQ=255)
                     tAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTTCCTTTGTGCACACGAGAAGGCGCCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCgg                                                                                      <  2:79389/140‑1 (MQ=255)
                      aaCGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCGCCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGa                                                                                     >  1:474669/1‑140 (MQ=255)
                           tCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCGCCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTc                                                                                >  1:514288/1‑140 (MQ=255)
                           tCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCGCCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTc                                                                                >  1:203044/1‑140 (MQ=255)
                                    gACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCGCCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAg                                                                       <  2:203044/140‑1 (MQ=255)
                                    gACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCGCCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAg                                                                       <  2:514288/140‑1 (MQ=255)
                                        tcatcaGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCGCCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAg                                                                   <  2:63989/140‑1 (MQ=255)
                                                agcTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCGCCAGGTACTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTc                                                           <  2:474669/140‑1 (MQ=255)
                                                    gTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCGCCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATcc                                                       >  2:110281/1‑140 (MQ=255)
                                                      ttGAACAACTGCGGGGATTGCGGCAGTGCGTAGANTTTACCTTTGTGCACACGAGAAGGCGCCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATCCAt                                                     >  1:134487/1‑140 (MQ=255)
                                                            aacTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCGCCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATCCATAAAACg                                               >  2:32700/1‑140 (MQ=255)
                                                                        tGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCGCCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATCCATAAAACGGCGCACCAGGCt                                   >  1:67355/1‑140 (MQ=255)
                                                                        tGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCGCCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATCCATAAAACGGCGCACCAGGCt                                   >  2:118942/1‑140 (MQ=255)
                                                                                                   tgCACACGAGAAGGCGCCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATCCATAAAACGGCGCACCAGGCTGGTGATTTTAGCGCGGGTTTTCAGgcg        >  2:129827/1‑140 (MQ=255)
                                                                                                        gcGAGAAGGCGCCAGGTAGTAACGCGCGACTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCAGTGGTCCTCCATAAAACGGCGCACCCGGCTGGTGATTCTAGCGCGGGTTTTCAGGCGCTggg   <  2:537883/139‑3 (MQ=255)
                                                                                                           agaAGGCGCCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATCCATAAAACGGCGCACCAGGCTGGTGATTTTAGCGCGGGTTTTCAGGCGCTGAGc  >  2:264584/1‑138 (MQ=255)
                                                                                                           agaAGGCGCCAGGTAGTCACGCGCGCCTTCCGGCGTGGATTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATCCATAAAACGGCGCACCAGGCTGGTGATTTTAGCGCGGGTTTTCAGGCGCTGAGc  <  1:264584/138‑1 (MQ=255)
                                                                                                                  |                                                                                                                                  
AAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATCCATAAAACGGCGCACCAGGCTGGTGATTTTAGCGCGGGTTTTCAGGCGCTGAGC  >  NC_000913/1949876‑1950120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A