Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	176	31.5	1082462	98.8%	1069472	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,225,973	T→C	V58A (GTG→GCG)	yohD →	DedA family protein YohD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,225,973	0	T	C	100.0%	119.1 / NA	33	V58A (GTG→GCG)	yohD	DedA family protein YohD
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/14); total (19/14)

TGCCGCGCTGGTGATCGGTAGCCTGGCGGAAGGTGAAACCGTGACTTTGCTGGGAGGCGTTGCGGCGCATCAGGGGCTATTAAAGTTCCCGCTGGTGGTACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCT  >  NC_000913/2225836‑2226101

tGCCGCGCTGGTGATCGGTAGCCTGGCGGAAGGTGAAACCGTGACTTTGCTGGGAGGCGTTGCGGCGCATCAGGGGCTATTAAAGTTCCCGCTGGTGGTACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGgcgc                                                                                                                                >  2:291123/1‑140 (MQ=255)

tGCCGCGCTGGTGATCGGTAGCCTGGCGGAAGGTGAAACCGTGACTTTGCTGGGAGGCGTTGCGGCGCATCAGGGGCTATTAAAGTTCCCGCTGGTGGTACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGgcgc                                                                                                                                >  1:419662/1‑140 (MQ=255)

  ccGCGCTGGTGATCGGTAGCCTGGCGGAAGGTGAAACCGTGACTTTGCTGGGAGGCGTTGCGGCGCATCAGGGGCTATTAAAGTTCCCGCTGGTGGTACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGCGctc                                                                                                                              >  1:217171/1‑140 (MQ=255)

  ccGCGCTGGTGATCGGTAGCCTGGCGGAAGGTGAAACCGTGACTTTGCTGGGAGGCGTTGCGGCGCATCAGGGGCTATTAAAGTTCCCGCTGGTGGTACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGCGctc                                                                                                                              >  2:185695/1‑140 (MQ=255)

     cgcTGGTGATCGGTAGCCTGGCGGAAGGTGAAACCGTGACTTTGCTGGGAGGCGTTGCGGCGCATCAGGGGCTATTAAAGTTCCCGCTGGTGGTACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGCGCTCTAt                                                                                                                           >  2:90949/1‑140 (MQ=255)

     cgcTGGTGATCGGTAGCCTGGCGGAAGGTGAAACCGTGACTTTGCTGGGAGGCGTTGCGGCGCATCAGGGGCTATTAAAGTTCCCGCTGGTGGTACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGCGCTCTAt                                                                                                                           >  1:216260/1‑140 (MQ=255)

      gcTGGTGATCGGTAGCCTGGCGGAAGGTGAAACCGTGACTTTGCTGGGAGGCGTTGCGGCGCATCAGGGGCTATTAAAGTTCCCGCTGGTGGTACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGCGCTCTATc                                                                                                                          >  2:493591/1‑140 (MQ=255)

       cTGGTGATCGGTAG‑CTGGCGGAAGGTGAAACCGTGACTTTGCTGGGAGGCGTTGCGGCGCATCAGGGGCTATTAAAGTTCCCGCTGGTGGTACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGCGCTCTATCtg                                                                                                                        >  2:27384/1‑140 (MQ=255)

                    gCCTGGCGGAAGGTGAAACCGTGACTTTGCTGGGAGGCGTTGCGGCGCATCAGGGGCTATTAAAGTTCCCGCTGGTGGTACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGCGCTCTATCTGTGCGGGcggcgg                                                                                                            >  1:364414/1‑140 (MQ=255)

                          cGGAAGGTGAAACCGTGACTTTGCTGGGAGGCGTTGCGGCGCATCAGGGGCTATTAAAGTTCCCGCTGGTGGTACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGCGCTCTATCTGTGCGGGCGGCGGTTTggc                                                                                                      >  2:335700/1‑140 (MQ=255)

                                             tttGCTGGGAGGCGTTGCGGCGCATCAGGGGCTATTAAAGTTCCCGCTGGTGGTACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGCGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGtt                                                                                   >  1:41018/1‑140 (MQ=255)

                                              ttGCTGGGAGGCGTTGCGGCGCATCAGGGGCTATTAAAGTTCCCGCTGGTGGTACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGCGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGttt                                                                                  <  2:261255/140‑1 (MQ=255)

                                                        gCGTTGCGGCGCATCAGGGGCTATTAAAGTTCCCGCTGGTGGTACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGCGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACAt                                                                        <  1:345259/140‑1 (MQ=255)

                                                         cGTTGCGGCGCATCAGGGGCTATTAAAGTTCCCGCTGGTGGTACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGCGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATc                                                                       >  2:319546/1‑140 (MQ=255)

                                                              cggcgCATCAGGGGCTATTAAAGTTCCCGCTGGTGGTACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGCGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACAt                                                                        <  1:1738/134‑1 (MQ=255)

                                                              cggcgCATCAGGGGCTATTAAAGTTCCCGCTGGTGGTACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGCGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACAt                                                                        >  2:1738/1‑134 (MQ=255)

                                                                     tCAGGGGCTATTAAAGTTCCCGCTGGTGGTACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGCGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTg                                                           >  2:281342/1‑140 (MQ=255)

                                                                         gggCTATTAAAGTTCCCGCTGGTGGTACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGCGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCg                                                       <  2:292493/140‑1 (MQ=255)

                                                                                       cccGCTGGTGGTACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGCGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTa                                         >  2:312829/1‑140 (MQ=255)

                                                                                          gCTGGTGGTACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGCGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATcc                                      <  1:312829/140‑1 (MQ=255)

                                                                                              gtggtACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGCGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACg                                  <  2:217171/140‑1 (MQ=255)

                                                                                              gtggtACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGCGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACg                                  >  2:344535/1‑140 (MQ=255)

                                                                                                ggtACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGCGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGcc                                <  2:419662/140‑1 (MQ=255)

                                                                                                 gtACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGCGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCGAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCa                               >  1:341869/1‑140 (MQ=255)

                                                                                                  tACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGCGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCGAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCAt                              <  2:341869/140‑1 (MQ=255)

                                                                                                      ttCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGCGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGt                          >  2:362109/1‑140 (MQ=255)

                                                                                                        cTGTGGCGCTTGGCGGCATGATTGGCGACCAGGCGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTAt                        <  1:319546/140‑1 (MQ=255)

                                                                                                            ggCGCTTGGCGGCATGATTGGCGACCAGGCGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGt                    >  2:492216/1‑140 (MQ=255)

                                                                                                               gcTTGGCGGCATGATTGGCGACCAGGCGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTg                 >  2:30671/1‑140 (MQ=255)

                                                                                                                cTTGGCGGCATGATTGGCGACCAGGCGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGt                >  2:273384/1‑140 (MQ=255)

                                                                                                                        cATGATTGGCGACCAGGCGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTa        <  1:344535/140‑1 (MQ=255)

                                                                                                                        cATGATTGGCGACCAGGCGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTa        <  1:309352/140‑1 (MQ=255)

                                                                                                                              tGGCGACCAGGCGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCt  <  1:103136/140‑1 (MQ=255)

                                                                                                                              tGGCGACCAGGCGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCt  <  1:291123/140‑1 (MQ=255)

                                                                                                                              tGGCGACCAGGCGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCt  >  2:89875/1‑140 (MQ=255)

                                                                                                                              tGGCGACCAGGCGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCt  <  1:362109/140‑1 (MQ=255)

TGCCGCGCTGGTGATCGGTAGCCTGGCGGAAGGTGAAACCGTGACTTTGCTGGGAGGCGTTGCGGCGCATCAGGGGCTATTAAAGTTCCCGCTGGTGGTACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCT  >  NC_000913/2225836‑2226101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A