Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F28 I1 R1 176 31.5 1082462 98.8% 1069472 138.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,376,357 T→C T155A (ACT→GCT)  menB ← 1,4‑dihydroxy‑2‑naphthoyl‑CoA synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,376,3570TC100.0% 110.1 / NA 31T155A (ACT→GCT) menB1,4‑dihydroxy‑2‑naphthoyl‑CoA synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/15);  total (16/15)

CCATATCCAGCGCCTGTTTTGCGTCGTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGTCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAG  >  NC_000913/2376225‑2376490
                                                                                                                                    |                                                                                                                                     
ccATATCCAGCGCCTGTTTTGCGTCGTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCg                                                                                                                                >  1:131728/1‑140 (MQ=255)
         gcgcCTGTTTTGCGTCGTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCa                                                                                                                       >  1:271795/1‑140 (MQ=255)
         gcgcCTGTTTTGCGTCCTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCa                                                                                                                       >  1:416786/1‑140 (MQ=255)
           gcCTGTTTTGCGTCGTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCAtt                                                                                                                     >  2:246743/1‑140 (MQ=255)
                ttttGCGTCGTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTg                                                                                                                >  2:375707/1‑140 (MQ=255)
                ttttGCGTCGTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTg                                                                                                                <  1:312599/140‑1 (MQ=255)
                ttttGCGTCGTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTg                                                                                                                <  2:197363/140‑1 (MQ=255)
                ttttGCGTCGTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTg                                                                                                                <  1:375707/140‑1 (MQ=255)
                ttttGCGTCCTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTg                                                                                                                <  2:416786/140‑1 (MQ=255)
                     cgtcgtACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTGCcgcg                                                                                                           <  2:519107/140‑1 (MQ=255)
                           aCTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTc                                                                                                     <  2:271795/140‑1 (MQ=255)
                                  gCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGc                                                                                              >  1:159933/1‑140 (MQ=255)
                                    acaGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGcac                                                                                            >  1:201098/1‑140 (MQ=255)
                                                     cgcgCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCAtgtg                                                                                    <  1:407956/131‑1 (MQ=255)
                                                     cgcgCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCAtgtg                                                                                    >  2:407956/1‑131 (MQ=255)
                                                               tCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGAccgccgccg                                                                 <  1:48661/140‑1 (MQ=255)
                                                               tCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGAccgccgccg                                                                 <  1:246743/140‑1 (MQ=255)
                                                                   cccGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATgg                                                             >  2:145746/1‑140 (MQ=255)
                                                                      gacgaTGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGt                                                          <  2:201098/140‑1 (MQ=255)
                                                                                          aaGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACa                                      <  2:347528/140‑1 (MQ=255)
                                                                                            gcgcCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAAc                                    >  1:441466/1‑140 (MQ=255)
                                                                                              gcCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCg                                  >  2:336163/1‑140 (MQ=255)
                                                                                               ccccAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGACATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCgg                                 >  2:18388/1‑140 (MQ=255)
                                                                                                cccAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGCCAACCGGt                                >  1:339429/1‑140 (MQ=255)
                                                                                                   aGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTc                             >  2:238639/1‑140 (MQ=255)
                                                                                                      cgccgTCGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGa                          <  1:336163/140‑1 (MQ=255)
                                                                                                            cGAAGGAACCGACTTTCGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGGTa                    <  1:238639/140‑1 (MQ=255)
                                                                                                                           tCGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGGTACGGATCTGACGCTgg     <  2:159933/140‑1 (MQ=255)
                                                                                                                            cGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGGGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGGTa                    <  2:275831/124‑1 (MQ=255)
                                                                                                                            cGGGCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGGTa                    >  1:275831/1‑124 (MQ=255)
                                                                                                                              ggCCAGCCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAg  >  2:185525/1‑140 (MQ=255)
                                                                                                                                    |                                                                                                                                     
CCATATCCAGCGCCTGTTTTGCGTCGTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAGGAACCGACTTTCGGGCCAGTCTGACCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAG  >  NC_000913/2376225‑2376490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A