Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	176	31.5	1082462	98.8%	1069472	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,477,075	T→C	R185R (AGA→AGG)	dsdC ←	DNA‑binding transcriptional dual regulator DsdC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,477,075	0	T	C	100.0%	122.3 / NA	34	R185R (AGA→AGG)	dsdC	DNA‑binding transcriptional dual regulator DsdC
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/15); total (19/15)

GAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCAT  >  NC_000913/2476938‑2477211

gaagaTGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCt                                                                                                                                        >  1:378466/1‑140 (MQ=255)

 aagaTGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCtt                                                                                                                                       >  1:74200/1‑140 (MQ=255)

 aagaTGTCGGCAAACTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCtt                                                                                                                                       <  2:505861/140‑1 (MQ=255)

  agaTGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCttt                                                                                                                                      <  2:74200/140‑1 (MQ=255)

      gTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGc                                                                                                                                  >  1:49337/1‑140 (MQ=255)

        cGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGt                                                                                                                                >  1:294277/1‑140 (MQ=255)

                tAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCggg                                                                                                                        <  2:378466/140‑1 (MQ=255)

                        tAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTcc                                                                                                                           >  2:406498/1‑129 (MQ=255)

                        tAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTcc                                                                                                                           <  1:406498/129‑1 (MQ=255)

                           tGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCggg                                                                                                                        >  1:284577/1‑129 (MQ=255)

                           tGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCggg                                                                                                                        <  2:284577/129‑1 (MQ=255)

                                  cccAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGAtt                                                                                                      >  1:403417/1‑140 (MQ=255)

                                              aTTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTcatccatc                                                                                          <  2:330311/140‑1 (MQ=255)

                                                tCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAg                                                                                        <  2:49337/140‑1 (MQ=255)

                                                          cGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGtgatga                                                                              <  2:175680/140‑1 (MQ=255)

                                                            gAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGtga                                                                                 >  2:350223/1‑135 (MQ=255)

                                                            gAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGtga                                                                                 <  1:350223/135‑1 (MQ=255)

                                                                                tctgtcATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGGATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAATGAGTTGCGCTGACGGCGcat                                                        >  2:491778/1‑140 (MQ=255)

                                                                                   gtcATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGcatcat                                                     <  2:430875/140‑1 (MQ=255)

                                                                                            cAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGa                                            >  1:126526/1‑140 (MQ=255)

                                                                                              aCGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATc                                          >  2:21210/1‑140 (MQ=255)

                                                                                              aCGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATc                                          >  1:470736/1‑140 (MQ=255)

                                                                                                gTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGc                                        <  2:126526/140‑1 (MQ=255)

                                                                                                        acacAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGa                                >  2:526630/1‑140 (MQ=255)

                                                                                                         cacaGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGAt                               >  2:484277/1‑140 (MQ=255)

                                                                                                         cacaGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGAt                               >  1:207449/1‑140 (MQ=255)

                                                                                                         cacaGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGAt                               >  1:314324/1‑140 (MQ=255)

                                                                                                             ggTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGAttacg                           <  2:470736/140‑4 (MQ=255)

                                                                                                                        gtgtTGGTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAg                >  2:42197/1‑140 (MQ=255)

                                                                                                                              gTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGAc          >  1:211572/1‑140 (MQ=255)

                                                                                                                               ttAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACg         <  2:12717/140‑1 (MQ=255)

                                                                                                                                tAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGt        >  2:420092/1‑140 (MQ=255)

                                                                                                                                 aaaGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGtt       <  2:211572/140‑1 (MQ=255)

                                                                                                                                      aTGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCAt  <  1:526630/140‑1 (MQ=255)

GAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCAT  >  NC_000913/2476938‑2477211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A