Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	176	31.5	1082462	98.8%	1069472	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,497,028	G→A	intergenic (+36/+29)	ypdK → / ← alaC	putative membrane protein YpdK/glutamate‑‑pyruvate aminotransferase AlaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,497,028	0	G	A	100.0%	124.7 / NA	37	intergenic (+36/+29)	ypdK/alaC	putative membrane protein YpdK/glutamate‑‑pyruvate aminotransferase AlaC
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (23/14); total (23/14)

CAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTT  >  NC_000913/2496896‑2497162

cAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGt                                                                                                                                 >  1:460236/1‑140 (MQ=255)

                agaTGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTgacgac                                                                                                                 <  1:243482/140‑1 (MQ=255)

                agaTGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTgacgac                                                                                                                 <  2:26753/140‑1 (MQ=255)

                 gaTGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGacgacg                                                                                                                >  1:28143/1‑140 (MQ=255)

                           gAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTc                                                                                                      >  2:524567/1‑140 (MQ=255)

                            aaaTATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTgacgac                                                                                                                 <  2:462913/128‑1 (MQ=255)

                            aaaTATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTgacgac                                                                                                                 >  1:462913/1‑128 (MQ=255)

                            aaaTATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTcc                                                                                                     >  2:482801/1‑140 (MQ=255)

                            aaaTATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTcc                                                                                                     >  2:42411/1‑140 (MQ=255)

                             aaTATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTAtt                                                                                                       <  1:957/137‑1 (MQ=255)

                             aaTATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTAtt                                                                                                       >  2:957/1‑137 (MQ=255)

                                    cTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTc                                                                                             >  1:464711/1‑140 (MQ=255)

                                                         ggTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGc                                                                        <  1:195654/140‑1 (MQ=255)

                                                         ggTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGc                                                                        <  1:524567/140‑1 (MQ=255)

                                                         ggTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGc                                                                        <  2:171628/140‑1 (MQ=255)

                                                          gTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCg                                                                       >  1:232068/1‑140 (MQ=255)

                                                             aTCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCgggg                                                                    >  2:430635/1‑139 (MQ=255)

                                                                  ttGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTaa                                                               <  2:460236/140‑1 (MQ=255)

                                                                          gTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATG‑AAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGc                                                      >  1:437856/1‑140 (MQ=255)

                                                                            aCTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCa                                                     <  2:232068/140‑1 (MQ=255)

                                                                                ttGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGa                                                 >  1:68492/1‑140 (MQ=255)

                                                                                    ccTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACAt                                             <  1:486682/140‑1 (MQ=255)

                                                                                            tCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTaa                                     >  1:7429/1‑140 (MQ=255)

                                                                                              tAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATc                                   <  2:157103/140‑1 (MQ=255)

                                                                                               aaaaGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATcc                                  <  1:369978/140‑1 (MQ=255)

                                                                                                      aaCTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGAt                           >  1:22842/1‑140 (MQ=255)

                                                                                                       aCTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTg                             <  1:323178/137‑1 (MQ=255)

                                                                                                       aCTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTg                             >  2:323178/1‑137 (MQ=255)

                                                                                                       aCTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATg                          >  1:449077/1‑140 (MQ=255)

                                                                                                       aCTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATg                          >  1:409113/1‑140 (MQ=255)

                                                                                                         tgtgCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGc                        <  2:68492/140‑1 (MQ=255)

                                                                                                          gtgCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGGAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGcc                       >  1:109463/1‑140 (MQ=255)

                                                                                                           tgCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCt                      >  2:22042/1‑140 (MQ=255)

                                                                                                                     aaCAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACg            >  2:295898/1‑140 (MQ=255)

                                                                                                                     aaCAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACACCGCCTTAATCCCCCTGGTGGCCTGACGAATACg            >  2:471306/1‑140 (MQ=255)

                                                                                                                               aTCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGttt  >  2:48650/1‑140 (MQ=255)

                                                                                                                               aTCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGGAAACCGTCGGCACGGGACAACGCCTTTATCCCTCTGATGGCCTGGCGAATACGGTGGCGGgtt  >  2:418823/1‑140 (MQ=255)

CAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTT  >  NC_000913/2496896‑2497162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A