Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	176	31.5	1082462	98.8%	1069472	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,723,931	A→G	E402G (GAG→GGG)	pssA →	phosphatidylserine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,723,931	0	A	G	97.3%	125.9 / ‑4.4	37	E402G (GAG→GGG)	pssA	phosphatidylserine synthase
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (20/16); total (21/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

CACCTGAAAGGGATGTGGGTTGATGATAAGTGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCAT  >  NC_000913/2723795‑2724054

cACCTGAAAGGGATGTGGGTTGATGATAAGTGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGggaa                                                                                                                          <  1:22118/140‑1 (MQ=255)

      aaaGGGATGTGGGTTGATGATAAGTGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGGGAAAGAACt                                                                                                                    >  2:218046/1‑140 (MQ=255)

          ggATGTGGGTTGATGATAAGTGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGGGAAAGAACTGGAg                                                                                                                <  2:416551/140‑1 (MQ=255)

                     gatgatAAGTGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGGGAAAGAACTGGAGCTGATCCGCGa                                                                                                     >  1:450884/1‑140 (MQ=255)

                      atgatAAGTGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGGGAAAGAACTGGAGCTGATCCGCGAg                                                                                                    >  1:101715/1‑140 (MQ=255)

                                gATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGGGAAAGAACTGGAGCTGATCCGCGAGCATAccacca                                                                                          >  1:168091/1‑140 (MQ=255)

                                gATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGGGAAAGAACTGGAGCTGATCCGCGAGCATAccacca                                                                                          >  2:338280/1‑140 (MQ=255)

                                gATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGGGAAAGAACTGGAGCTGATCCGCGAGCATAccacca                                                                                          <  2:168091/140‑1 (MQ=255)

                                      gATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGGGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTa                                                                                    >  2:14998/1‑140 (MQ=255)

                                       aTCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGGGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTaa                                                                                   >  2:85822/1‑140 (MQ=255)

                                        tCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGGGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAg                                                                                  <  1:85822/140‑1 (MQ=255)

                                           ccGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGGGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCAc                                                                               >  2:330629/1‑140 (MQ=255)

                                            cGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGGGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACt                                                                              >  2:359564/1‑140 (MQ=255)

                                                  taaCCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGGGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATcgcg                                                                        <  2:101715/140‑1 (MQ=255)

                                                  taaCCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGGGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATcgcg                                                                        <  2:362820/140‑1 (MQ=255)

                                                        gAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGGGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGc                                                                  >  1:508761/1‑140 (MQ=255)

                                                         aaCCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGGGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAg                                                                                  >  2:80940/1‑123 (MQ=255)

                                                         aaCCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGGGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAg                                                                                  <  1:80940/123‑1 (MQ=255)

                                                         aaCCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGGGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCa                                                                 >  1:345154/1‑140 (MQ=255)

                                                               cgcgcCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGGGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGAt                                                                      <  1:414111/129‑1 (MQ=255)

                                                               cgcgcCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGGGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGAt                                                                      >  2:414111/1‑129 (MQ=255)

                                                               cgcgcCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGGGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCa                                                                 <  1:111765/134‑1 (MQ=255)

                                                               cgcgcCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGGGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCa                                                                 >  2:111765/1‑134 (MQ=255)

                                                               cgcgcCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTAt                                                           >  2:241194/1‑140 (MQ=255)

                                                                gcgcCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGGGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTAtt                                                          >  1:39389/1‑140 (MQ=255)

                                                                   ccTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGGGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGcc                                                       >  2:182743/1‑140 (MQ=255)

                                                                     tGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGGGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGa                                                     <  1:218046/140‑1 (MQ=255)

                                                                                 ctggaAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGGGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGaa                                         <  2:323114/140‑1 (MQ=255)

                                                                                 ctggaAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGGGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGaa                                         <  2:377937/140‑1 (MQ=255)

                                                                                 ctggaAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGGGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGaa                                         <  1:359564/140‑1 (MQ=255)

                                                                                        aCGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGGGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGt                                  >  1:235306/1‑140 (MQ=255)

                                                                                                        cGATCCGCAACTTGAGCTGGCGCCACAGCGAGGGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGtt                  >  1:2601/1‑140 (MQ=255)

                                                                                                           tCCGCAACTTGAGCTGACGCCACAGCGAGGGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTgc               <  2:515068/140‑1 (MQ=255)

                                                                                                             cGCAACTTGAGCTGGCGCCACAGCGAGGGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTgcgc             <  1:338280/140‑1 (MQ=255)

                                                                                                             cGCAACTTGAGCTGGCGCCACAGCGAGGGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTgcgc             <  2:39389/140‑1 (MQ=255)

                                                                                                                     gAGCTGGCGCCACAGCGAGGGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCg     >  1:520434/1‑140 (MQ=255)

                                                                                                                     gAGCTGGCGCCACAGCGAGGGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCg     >  2:417087/1‑140 (MQ=255)

                                                                                                                      aGCTGGCGCCACAGCGAGGGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGc    >  2:430801/1‑140 (MQ=255)

                                                                                                                        cTGGCGCCACAGCGAGGGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCAt  <  2:508761/140‑1 (MQ=255)

CACCTGAAAGGGATGTGGGTTGATGATAAGTGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCAT  >  NC_000913/2723795‑2724054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A