Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F28 I1 R1
|
176 |
31.5 |
1082462 |
98.8% |
1069472 |
138.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,798,536 |
T→C |
intergenic (‑41/‑628) |
stpA ← / → alaE |
DNA‑binding transcriptional repressor StpA with RNA chaperone activity/L‑alanine exporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,798,536 | 0 | T | C | 100.0%
| 83.6
/ NA
| 24 | intergenic (‑41/‑628) | stpA/alaE | DNA‑binding transcriptional repressor StpA with RNA chaperone activity/L‑alanine exporter |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (11/13); total (11/13) |
TCGAGCATTTCTTCAAGAACGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAACAAAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAATCTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGAATAATTTCAAGATTATATATTAGCCATTTTGAT > NC_000913/2798404‑2798672
|
tCGAGCATTTCTTCAAGAACGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAACAAAAAAAGTATTCCGTcgcg < 2:277141/140‑1 (MQ=255)
aTTTCTTCAAGAACGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAACAAAAAAAGTATTCCGTCGCGTAgtgt < 1:529537/140‑1 (MQ=255)
cAAGAACGCCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAACAAAAAAAGTATTCCGTCGCGTAGTGTAAGCGAt < 1:93577/140‑1 (MQ=255)
aTTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAACAAAAAAAGTATTCCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCcac > 1:141884/1‑140 (MQ=255)
aTCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAACAAAAAAAGTATTCCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAATc > 1:432459/1‑140 (MQ=255)
tGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAACAAAAAAAGTATTCCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAATCTATTTACAGTCCTGCGCga > 2:511892/1‑140 (MQ=255)
tGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAACAAAAAAAGTATTCCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAATCTATTTACAGTCCTGCGCGAGATTATTTCAg < 2:67576/137‑1 (MQ=255)
tGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAACAAAAAAAGTATTCCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAATCTATTTACAGTCCTGCGCGAGATTATTTCAg > 1:67576/1‑137 (MQ=255)
tGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAACAAAAAAAGTATTCCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAATCTATTTACAGTCCTGCGCGAGATTATTTCAGCTg > 2:389657/1‑140 (MQ=255)
cGGACATAATAAAGAAAACCTTTTAACGCCAAAACAAAAAAAGTATTCCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAATCTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTg > 1:170156/1‑140 (MQ=255)
aCATAATAAAGAAAACCTTTTAACGCCAAAACAAAAAAAGTATTCCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAATCTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGaaa < 2:170156/140‑1 (MQ=255)
aataaAGAAAACCTTTTAACGCCAAAACAAAAAAAGTATTCCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAATCTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGa > 1:400335/1‑134 (MQ=255)
aataaAGAAAACCTTTTAACGCCAAAACAAAAAAAGTATTCCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAATCTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGa < 2:400335/134‑1 (MQ=255)
aataaAGAAAACCTTTTAACGCCAAAACAAAAAAAGTATTCCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAATCTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAActct < 1:444785/140‑1 (MQ=255)
aataaAGAAAACCTTTTAACGCCAAAACAAAAAAAGTATTCCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAATCTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAActct < 1:209910/140‑1 (MQ=255)
ataaAGAAAACCTTTTAACGCCAAAACAAAAAAAGTATTCCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAATCTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACtctc > 2:52630/1‑140 (MQ=255)
gAAAACCTTTTAACGCCAAAACAAAAAAAGTATTCCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAATCTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGaat < 2:235864/140‑1 (MQ=255)
aaaCCTTTTAACGCCAAAACAAAAAAAGTATTCCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAATCTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGaataa > 2:536977/1‑140 (MQ=255)
aCCTTTTAACGCCAAAACAAAAAAAGTATTCCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAATCTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGAATAAtt > 1:231009/1‑140 (MQ=255)
ccTTTTAACGCCAAAACAAAAAAAGTATTCCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAATCTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGAATAAttt < 2:427685/140‑1 (MQ=255)
cTTTTAACGCCAAAACAAAAAAAGTATTCCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAATCTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGAATAATTTc < 2:535016/140‑1 (MQ=255)
ttttAACGCCAAAACAAAAAAAGTATTCCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAATCTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGAATAATTTCa < 2:231009/140‑1 (MQ=255)
ccAAAACAAAAAAAGTATTCCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAATCTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGAATAATTTCAAGATtata > 2:410744/1‑140 (MQ=255)
aTTCCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAATCTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGAATAATTTCAAGATTATATATTAGCCATTTtgat < 2:141884/140‑1 (MQ=255)
|
TCGAGCATTTCTTCAAGAACGTCAATGGAGAATTCGCGAGCCATCGCACGGAGGGTGCGAATGTTATTTAAACTTTGTAACATTACGGACATAATAAAGAAAACCTTTTAACGCCAAAACAAAAAAAGTATTTCGTCGCGTAGTGTAAGCGATCACTTATTATATTTCCACAAAATTTAATCTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGAATAATTTCAAGATTATATATTAGCCATTTTGAT > NC_000913/2798404‑2798672
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
