Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	176	31.5	1082462	98.8%	1069472	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,803,182	G→A	R612H (CGC→CAC)	nrdE →	ribonucleoside‑diphosphate reductase 2 subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,803,182	0	G	A	100.0%	121.6 / NA	36	R612H (CGC→CAC)	nrdE	ribonucleoside‑diphosphate reductase 2 subunit alpha
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/17); total (19/17)

CGCAGCTGCGCGACGACGTGATGCGCTACGGCATATACAACCAGAATCTTCAGGCGGTGCCGCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACGCAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATG  >  NC_000913/2803047‑2803316

cgcAGCTGCGCGACGACGTGATGCGCTACGGCATATACAACCAGAATCTTCAGGCGGTGCCGCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACaaa                                                                                                                                    <  2:366828/140‑1 (MQ=255)

   aGCTGCGCGACGACGTGATGCGCTACGGCATATACAACCAGAATCTTCAGGCGGTGCCGCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAaga                                                                                                                                  >  1:223217/1‑139 (MQ=255)

   aGCTGCGCGACGACGTGATGCGCTACGGCATATACAACCAGAATCTTCAGGCGGTGCCGCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAaga                                                                                                                                  <  2:223217/139‑1 (MQ=255)

          cgacgaCGTGATGCGCTACGGCATATACAACCAGAATCTTCAGGCGGTGCCGCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCaaaa                                                                                                                          >  1:492496/1‑140 (MQ=255)

                  tGATGCGCTACGGCATATACAACCAGAATCTTCAGGCGGTGCCGCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGAcgc                                                                                                                  <  2:427737/140‑1 (MQ=255)

                                 tataCAACCAGAATCTTCAGGCGGTGCCGCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGcc                                                                                                   >  2:347723/1‑140 (MQ=255)

                                 tataCAACCAGAATCTTCAGGCGGTGCCGCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGcc                                                                                                   >  1:507185/1‑140 (MQ=255)

                                      aaCCAGAATCTTCAGGCGGTGCCGCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGtt                                                                                              >  1:137508/1‑140 (MQ=255)

                                       aCCAGAATCTTCAGGCGGTGCCGCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGttt                                                                                             >  1:423463/1‑140 (MQ=255)

                                           gAATCTTCAGGCGGTGCCGCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGa                                                                                         >  2:153981/1‑140 (MQ=255)

                                            aaTCTTCAGGCGGTGCCGCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGAc                                                                                        >  1:402594/1‑140 (MQ=255)

                                            aaTCTTCAGGCGGTGCCGCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGAc                                                                                        >  1:493983/1‑140 (MQ=255)

                                                        gTGCCGCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCt                                                                            >  1:392691/1‑140 (MQ=255)

                                                          gccgccAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTgg                                                                          <  2:392691/140‑1 (MQ=255)

                                                            cgccAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGgcg                                                                        <  1:153981/140‑1 (MQ=255)

                                                            cgccAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGgcg                                                                        <  2:423463/140‑1 (MQ=255)

                                                            cgccAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGgcg                                                                        <  1:237257/140‑1 (MQ=255)

                                                                                tataTCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGa                                                    >  1:25421/1‑140 (MQ=255)

                                                                                     cAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTg                                               >  1:22/1‑140 (MQ=255)

                                                                                     cAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTg                                               <  2:385940/140‑1 (MQ=255)

                                                                                       aCCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGgc                                             >  2:488746/1‑140 (MQ=255)

                                                                                       aCCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGgc                                             >  2:416457/1‑140 (MQ=255)

                                                                                         cATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGgcgc                                           >  1:22176/1‑140 (MQ=255)

                                                                                          aTGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCa                                          <  1:416457/140‑1 (MQ=255)

                                                                                          aTGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCa                                          <  2:402594/140‑1 (MQ=255)

                                                                                                 gTCGAGTATTCATCCGATTGTCGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGa                                   <  2:492496/140‑1 (MQ=255)

                                                                                                  tCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGat                                  >  1:345346/1‑140 (MQ=255)

                                                                                                      gTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGatcatc                              >  1:27320/1‑140 (MQ=255)

                                                                                                        aTTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGa                            <  2:345346/140‑1 (MQ=255)

                                                                                                                gATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTAcg                    <  1:68421/140‑1 (MQ=255)

                                                                                                                   tgtgGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAACTGGCGCAGAAAAGATCATCGACACCTACGCgg                 >  2:398826/1‑140 (MQ=255)

                                                                                                                     tgGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGaa               <  2:22/140‑1 (MQ=255)

                                                                                                                       gCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGc             >  1:16339/1‑140 (MQ=255)

                                                                                                                            aGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTc        <  1:488746/140‑1 (MQ=255)

                                                                                                                               agagATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGcc     <  1:300159/140‑1 (MQ=255)

                                                                                                                                  gaTACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATg  <  2:16339/140‑1 (MQ=255)

CGCAGCTGCGCGACGACGTGATGCGCTACGGCATATACAACCAGAATCTTCAGGCGGTGCCGCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACGCAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATG  >  NC_000913/2803047‑2803316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A