Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	176	31.5	1082462	98.8%	1069472	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,182,227	T→C	T70A (ACG→GCG)	ygiD ←	4,5‑DOPA dioxygenase extradiol

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,182,227	0	T	C	100.0%	90.9 / NA	26	T70A (ACG→GCG)	ygiD	4,5‑DOPA dioxygenase extradiol
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (16/10); total (16/10)

CTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCTGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGTCTCCATCGCGGTCACTCCTGTTCCACGGGTAAACCAGTGAGCCGAAACCACCACAATCGCTTGCGGGCGTGGCAATGTCATCCCCAACTTCTGCCAGCTGCGGGTATACAAATTATCTTCC  >  NC_000913/3182092‑3182348

cttTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCTGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGCCTcc                                                                                                                       >  1:473973/1‑140 (MQ=255)

 tttATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCTGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGCCTCCa                                                                                                                      >  2:195847/1‑140 (MQ=255)

          cGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCTGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGcct                                                                                                                         <  1:520087/128‑1 (MQ=255)

          cGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCTGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGcct                                                                                                                         >  2:520087/1‑128 (MQ=255)

          cGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCTGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGCCTCCATc                                                                                                                    <  1:327118/133‑1 (MQ=255)

          cGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCTGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGCCTCCATc                                                                                                                    >  2:327118/1‑133 (MQ=255)

            tCACCGGGATCGGCGCTAACAGCTCAACCAGACGCTGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGCCTCCATCGCGGTCACt                                                                                                           <  1:195847/140‑1 (MQ=255)

                                aGCTCAACCAGACGCTGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGCCTCCATCGCGGTCACTCCTGTTCCACGGGTAAACCa                                                                                       <  2:63335/140‑1 (MQ=255)

                                            cGCTGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGCCTCCATCGCGGTCACTCCTGTTCCACGGGTAAACCAGTGAGCCGAAac                                                                           >  1:482951/1‑140 (MQ=255)

                                                 tgCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGCCTCCATCGCGGTCACTCCTGTTCCACGGGTAAACCAGTGAGCCGAAAccacca                                                                      >  2:276657/1‑140 (MQ=255)

                                                 tgCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGCCTCCATCGCGGTCACTCCTGTTCCACGGGTAAACCAGTGAGCCGAAAccacca                                                                      >  2:296354/1‑140 (MQ=255)

                                                 tgCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGCCTCCATCGCGGTCACTCCTGTTCCACGGGTAAACCAGTGAGCCGAAAccacca                                                                      >  2:498968/1‑140 (MQ=255)

                                                         cAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGCCTCCATCGCGGTCACTCCTGTTCCACGGGTAAACCAGTGAGCCGAAACCACCACAATCGCt                                                              >  1:243200/1‑140 (MQ=255)

                                                                      aGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGCCTCCATCGCGGTCACTCCTGTTCCACGGGTAAACCAGTGAGCCGAAACCACCACAATCGCTTGCGGGCGTGGCa                                                 >  2:227247/1‑140 (MQ=255)

                                                                      aGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCATGGGCGGCGCCTCCATCGCGGTCACTCCTGTTCCACGGGTAAACCAGTGAGCCGAAACCACCACAATCGCTTGCGGGCGTGGCa                                                 >  2:281306/1‑140 (MQ=255)

                                                                           gATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGCCTCCATCGCGGTCACTCCTGTTCCACGGGTAAACCAGTGAGCCGAAACCACCACAATCGCTTGCGGGCGTGGCAATGTc                                            <  1:276657/140‑1 (MQ=255)

                                                                                tGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGCCTCCATCGCGGTCACTCCTGTTCCACGGGTAAACCAGTGAGCCGAAACCACCACAATCGCTTGCGGGCGTGGCAATGTCATccc                                       >  1:269665/1‑140 (MQ=255)

                                                                                     aTCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGCCTCCATCGCGGTCACTCCTGTTCCACGGGTAAACCAGTGAGCCGAAACCACCACAATCGCTTGCGGGCGTGGCAATGTCATCCCCAACt                                  >  1:248284/1‑140 (MQ=255)

                                                                                         tACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGCCTCCATCGCGGTCACTCCTGTTCCACGGGTAAACCAGTGAGCCGAAACCACCACAATCGCTTGCGGGCGTGGCAATGTCATCCCCAACTTCTg                              >  1:7034/1‑140 (MQ=255)

                                                                                         tACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGCCTCCATCGCGGTCACTCCTGTTCCACGGGTAAACCAGTGAGCCGAAACCACCACAATCGCTTGCGGGCGTGGCAATGTCATCCCCAACTTCTg                              <  1:498968/140‑1 (MQ=255)

                                                                                         tACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGCCTCCATCGCGGTCACTCCTGTTCCACGGGTAAACCAGTGAGCCGAAACCACCACAATCGCTTGCGGGCGTGGCAATGTCATCCCCAACTTCTg                              <  1:296354/140‑1 (MQ=255)

                                                                                                ccTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGCCTCCATCGCGGTCACTCCTGTTCCACGGGTAAACCAGTGAGCCGAAACCACCACAATCGCTTGCGGGCGTGGCAATGTCATCCCCAACTTCTGCCAGCTg                       >  1:474181/1‑140 (MQ=255)

                                                                                                ccTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGCCTCCATCGCGGTCACTCCTGTTCCACGGGTAAACCAGTGAGCCGAAACCACCACAATCGCTTGCGGGCGTGGCAATGTCATCCCCAACTTCTGCCAGCTg                       <  2:8619/140‑1 (MQ=255)

                                                                                                   gCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGCCTCCATCGCGGTCACTCCTGTTCCACGGGTAAACCAGTGAGCCGAAACCACCACAATCGCTTGCGGGCGTGGCAATGTCATCCCCAACTTCTGCCAGCTGc                      <  2:2969/138‑1 (MQ=255)

                                                                                                   gCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGCCTCCATCGCGGTCACTCCTGTTCCACGGGTAAACCAGTGAGCCGAAACCACCACAATCGCTTGCGGGCGTGGCAATGTCATCCCCAACTTCTGCCAGCTGc                      >  1:2969/1‑138 (MQ=255)

                                                                                                             accaAAGTCATGAATCGTGGGCGGCGCCTCCATCGCGGTCACTCCTGTTCCACGGGTAAACCAGTGAGCCGAAACCACCACAATCGCTTGCGGGCGTGGCAATGTCATCCCCAACTTCTGCCAGCTGCGGGTATACAAAt          <  2:7034/140‑1 (MQ=255)

                                                                                                                  aGTCATGAATCGTGGGCGGCGCCTCCATCGCGGTCACTCCTGTTCCACGGGTAAACCAGTGAGCCGAAACCACCACAATCGCTTGCGGGCGTGGCAATGTCATCCCCAACTTCTGCCAGCTGCGGGTATACAAATTATCt     <  2:269665/140‑1 (MQ=255)

                                                                                                                     cATGAATCGTGGGCGGCGCCTCCATCGCGGTCACTCCTGTTCCACGGGTAAACCAGTGAGCCGAAACCACCACAATCGCTTGCGGGCGTGGCAATGTCATCCCCAACTTCTGCCAGCTGCGGGTATACAAATTATCTTcc  >  1:448654/1‑140 (MQ=255)

CTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCTGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGTCTCCATCGCGGTCACTCCTGTTCCACGGGTAAACCAGTGAGCCGAAACCACCACAATCGCTTGCGGGCGTGGCAATGTCATCCCCAACTTCTGCCAGCTGCGGGTATACAAATTATCTTCC  >  NC_000913/3182092‑3182348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A