Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	176	31.5	1082462	98.8%	1069472	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,258,693	(C)_5→6	coding (957/1365 nt)	tdcG ←	L‑serine deaminase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,258,688	1	.	C	100.0%	122.9 / NA	34	A321G (GCT→GGT)	tdcG	L‑serine deaminase III
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (17/17); total (17/17)

TACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAG‑CCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCA  >  NC_000913/3258555‑3258819

tACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTACCCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAG‑cccccc                                                                                                                               <  1:453337/140‑2 (MQ=255)

    tGCGCCGGACTGCCGCCCAGTAGTTCAGTTACCCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAG‑ccccccgcgg                                                                                                                           <  2:417157/140‑6 (MQ=255)

    tGCGCCGGACTGCCGCCCAGGAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAG‑ccccccgcgg                                                                                                                           <  2:402082/140‑6 (MQ=255)

      cgcCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCCGCGGcc                                                                                                                          >  2:315422/1‑140 (MQ=255)

        ccGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCCGCGGCCAg                                                                                                                        >  1:420511/1‑140 (MQ=255)

                         agtTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCCGCGGCCAGAAAATAGCGGGCAATTg                                                                                                       <  2:81494/140‑1 (MQ=255)

                           ttCAGTTAGCCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCCGCGGCCAGAAAATAGCGGGCAATTGAc                                                                                                     <  1:365450/140‑1 (MQ=255)

                                        gccgccgccATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCg                                                                                        >  2:301111/1‑140 (MQ=255)

                                          cgccgccATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGga                                                                                      <  2:147482/140‑1 (MQ=255)

                                                      aCAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTa                                                                          <  1:56403/140‑1 (MQ=255)

                                                       cAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTAt                                                                         <  2:251367/140‑1 (MQ=255)

                                                          gCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGt                                                                      >  2:226168/1‑140 (MQ=255)

                                                            cACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGtaa                                                                    <  1:226168/140‑1 (MQ=255)

                                                            cACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGtaa                                                                    <  2:457458/140‑1 (MQ=255)

                                                               gCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGtaataa                                                                 >  1:220220/1‑140 (MQ=255)

                                                                    tctcCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAg                                                            >  1:159571/1‑140 (MQ=255)

                                                                         cccTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTg                                                       >  1:35817/1‑140 (MQ=255)

                                                                            tGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCg                                                    >  2:142564/1‑140 (MQ=255)

                                                                                aGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGt                                                           <  2:228461/129‑1 (MQ=255)

                                                                                aGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGt                                                           >  1:228461/1‑129 (MQ=255)

                                                                                  ccGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGaataa                                              >  2:81910/1‑140 (MQ=255)

                                                                                        tCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCcgc                                        >  2:462659/1‑140 (MQ=255)

                                                                                         ccGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCcgca                                       <  1:142564/140‑1 (MQ=255)

                                                                                         ccGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCcgca                                       <  2:255226/140‑1 (MQ=255)

                                                                                           gcgcCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCcgcacg                                     <  1:431706/140‑1 (MQ=255)

                                                                                                agagATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCg                                <  2:91056/140‑1 (MQ=255)

                                                                                                      ggAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTc                          <  1:315422/140‑1 (MQ=255)

                                                                                                        aGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCgg                        <  1:335402/140‑1 (MQ=255)

                                                                                                         ggCGTTCATTTTATACAGCGCGCCAATAGCCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGt                       >  2:407003/1‑140 (MQ=255)

                                                                                                             ttCATTTTATACAGCGCGCCAATAGCCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCg                   >  1:485955/1‑140 (MQ=255)

                                                                                                                     ataCAGCGCGCCAATAGCCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCAcg           <  1:301111/140‑1 (MQ=255)

                                                                                                                     ataCAGCGCGCCAATAGCCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCAcg           <  2:220220/140‑1 (MQ=255)

                                                                                                                           cgcgcCAATAGCCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGcgcccg     >  2:332964/1‑140 (MQ=255)

                                                                                                                           cgcgcCAATAGCCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGcgcccg     >  2:252591/1‑140 (MQ=255)

                                                                                                                           cgcgcCAATAGCCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGcgcccg     >  1:340499/1‑140 (MQ=255)

                                                                                                                           cgcgcCAATAGCCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGcgcccg     >  2:231589/1‑140 (MQ=255)

                                                                                                                              gcCAATAGCCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCa  <  1:332964/140‑1 (MQ=255)

TACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAG‑CCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCA  >  NC_000913/3258555‑3258819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A