Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F28 I1 R1
|
176 |
31.5 |
1082462 |
98.8% |
1069472 |
138.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,516,831 |
G→A |
P159L (CCG→CTG) |
damX ← |
cell division protein DamX |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,516,831 | 0 | G | A | 100.0%
| 85.0
/ NA
| 25 | P159L (CCG→CTG) | damX | cell division protein DamX |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/8); total (17/8) |
TTGTTCAGGTCACCCTGCACTTCAACACGTTGTTGACCATCCGTTGCCACCGGGGTTTGCCCTTGAGTCGGCGTAGAAGAGATCGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATC > NC_000913/3516699‑3516959
|
ttGTTCAGGTCACCCTGCACTTCAACACGTTGTTGACCATCCGTTGCCACCGGGGTTTGCCCTTGAGTCGGCGTAGAAGAGATCGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCgctgg > 1:423571/1‑140 (MQ=255)
ccTGCACTTCAACACGTTGTTGACCATCCGTTGCCACCGGGGTTTGCCCTTGAGTCGGCGTAGAAGAGATCGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTc > 1:357053/1‑140 (MQ=255)
aCTTCAACACGTTGTTGACCATCCGTTGCCACCGGGGTTTGCCCTTGAGTCGGCGTAGAAGAGATCGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCa > 2:85500/1‑132 (MQ=255)
aCTTCAACACGTTGTTGACCATCCGTTGCCACCGGGGTTTGCCCTTGAGTCGGCGTAGAAGAGATCGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCa < 1:85500/132‑1 (MQ=255)
aCTTCAACACGTTGTTGACCATCCGTTGCCACCGGGGTTTGCCCTTGAGTCGGCGTAGAAGAGATCGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTg > 2:202701/1‑140 (MQ=255)
aCTTCAACACGTTGTTGACCATCCGTTGCCACCGGGGTTTGCCCTTGAGTCGGCGTAGAAGAGATCGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTg > 1:515305/1‑140 (MQ=255)
cacGTTGTTGACCATCCGTTGCCACCGGGGTTTGCCCTTGAGTCGGCGTAGAAGAGATCGGTGGCAGAGAAACATCCTGCTGAGTAGTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTc > 2:470804/1‑140 (MQ=255)
tgACCATCCGTTGCCACCGGGGTTTGCCCTTGAGTCGGCGTAGAAGAGATCGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGcc < 2:357053/140‑1 (MQ=255)
aTCCGTTGCCACCGGGGTTTGCCCTTGAGTCGGCGTAGAAGAGATCGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCg < 1:260334/128‑1 (MQ=255)
aTCCGTTGCCACCGGGGTTTGCCCTTGAGTCGGCGTAGAAGAGATCGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCg > 2:260334/1‑128 (MQ=255)
ccGTTGCCACCGGGGTTTGCCCTTGAGTCGGCGTAGAAGAGATCGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGa > 2:32213/1‑140 (MQ=255)
ggggTTTGCCCTTGAGTCGGCGTAGAAGAGATCGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACtctt > 2:184437/1‑140 (MQ=255)
ccTTGAGTCGGCGTAGAAGAGATCGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGa < 1:470804/140‑1 (MQ=255)
ggCGTAGAAGAGATCGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTg < 1:427767/140‑1 (MQ=255)
tAGAAGAGATCGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCg < 2:423571/140‑1 (MQ=255)
aTCGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGt > 1:138154/1‑140 (MQ=255)
cGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTgg > 2:367507/1‑140 (MQ=255)
cGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTgg > 1:296830/1‑140 (MQ=255)
gCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTTGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAg < 1:126272/140‑1 (MQ=255)
tCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAg > 2:277010/1‑140 (MQ=255)
tCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAg > 2:218597/1‑140 (MQ=255)
tgctgAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAgcgc > 1:488199/1‑140 (MQ=255)
tgAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGa < 2:296830/140‑1 (MQ=255)
aGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAac > 2:478378/1‑140 (MQ=255)
gCAGGCGAGGCTCCCATCGCTGGGTGCACGCCATTCGACTGATTGGACGCATAGCCAGCAAGATCAATACTCTTATCGGCGGACGCGGTTTGAACGCTGGAAGTGGTCGCTGGGGCTTTTAGCGCAGAaccgataccgat > 2:421205/1‑140 (MQ=255)
aCGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATc > 1:301514/1‑140 (MQ=255)
|
TTGTTCAGGTCACCCTGCACTTCAACACGTTGTTGACCATCCGTTGCCACCGGGGTTTGCCCTTGAGTCGGCGTAGAAGAGATCGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATC > NC_000913/3516699‑3516959
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
